[Histonet] (no subject)

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[Histonet] modified Gallyas stain for microglia

Hello, I'm using the modified silver stain of Gallyas (J. Neurosci. Methods 50:159--164, 1993) for microglia. It didn't work first time round so I'm doing some trouble shooting. My main concern is the shelf life of the potassium ferroscyanide solution (1 g K3Fe(CN)6 + 0.1 mL H2SO4 per 100 mL). It

[Histonet] (no subject)

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Re: [Histonet] motorized cryostat

In spite of the apparent advantage, FS are so uniquely demanding while sectioning, that the manual touch could never be substituted by an automatic feeding mechanism. Buy a good manual cryostat. I always used Leica. Ask for a demo. René J. From: Ed Roy e...@illinois.edu To:

Re: [Histonet] Histology station for core lab

For teaching you are right that the path is manual. For a limited budget Google for used microtomes or try eBay. René J. From: Debra Baluch page.bal...@asu.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, May 13, 2011 7:16 PM Subject: [Histonet] Histology

[Histonet] Re:Hi. Alice asked me to help you to stop smoking! Don’t bother! I know how to help you!

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[Histonet] Re: modified Gallyas stain for microglia

Tyrone Genade asks: I'm using the modified silver stain of Gallyas (J. Neurosci. Methods 50:159--164, 1993) for microglia. It didn't work first time round so I'm doing some trouble shooting. My main concern is the shelf life of the potassium ferroscyanide solution (1 g K3Fe(CN)6 + 0.1 mL

[Histonet] Outdating- Embedding Wax and Mounting Media

To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their continued use beyond this date. I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and

RE: [Histonet] Outdating- Embedding Wax and Mounting Media

Now you guys have done it!!! All the QAQC gurus are thinking!!! Rae Staskiewicz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Saturday, May 14, 2011 4:56 PM To: John Smallwood;