RE: [Histonet] VENTANA ULTRA ER,PR,HER2

Hi Antoinette, We currently use Ventana's ER and PR on both XT and Ultra. From what I understand, the antibody dispenser is labeled IVD. We report all cases as being 'approved by the FDA for IVD use'. We do perform correlation/precision studies twice a year. For this study we run five cases on

RE: [Histonet] How many tissues an histo tech is suppose to cut per

Stella, Please keep us informed as to the outcome of your discussions with management. I hope it all works out in your favor. I have also been in the field over 35 yrs. and have never been known as one of the fastest sectioners, but I always focused on quality and thought about the patient

[Histonet] Quenching

Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks  I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Quenching

Candice: Quenching is usually done in IHC (immuno-histo-chemical) procedures and is intended to eliminate the reaction of the peroxidase (enzyme) present in all cells. If this step is not done the background (noise) in the form of dark color present in the final section could mask the actual

[Histonet] Could you please recommend?

Hi, I am in a research lab and currently we are looking into buying a good cryostat to work with human samples. I have always used a Leica model and currently we own a Leica 3050S. We would like get one that comes with decontamination feature (UV lamp etc...). I would like to know your

[Histonet] Could you please recommend? (Bascaramurty, Saro)

We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz, Leica and a unit so old I think it came with Noah on the boat don't even remember the maker. Leica is the favorite. It sections well

Re: [Histonet] Could you please recommend? (Bascaramurty, Saro)

I second the Leica! :o) Michelle Sent from my iPhone On Jun 29, 2011, at 1:14 PM, White, Lisa M. lisa.whi...@va.gov wrote: We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz,

[Histonet] microtome blade donations

Hi, One of our pathologists is going on a volunteer mission to a pathology lab in Malawi, Africa in a few weeks, and he is looking for donations of low profile microtome blades for the histology lab there. He said they will need around 200 blades or so, but any amount would help. He leaves

[Histonet] Billing for CD3/20

Hey all, We are looking at doing the double stain CD3/20 and wanted to know how everyone is billing for it. Can we bill for 2 immuno stains? Or do we have to bill for one? What is the proper CPT for doing double or even triple staining? We are a Medicare facility. Thanks Matt Lunetta BS

AW: [Histonet] Quenching

Quenching is also a term used with depressing fluorescence activity. This can happen in quantitative realtime-PCR procedures. Here a quencher is situated close to a fluorochrom and therefore the activity is low. During incorporation of the nucleotides the quencher is released and the

[Histonet] AUTO: is out of the office. (returning Mon 07/11/2011)

I am out of the office from Wed 06/29/2011 until Mon 07/11/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mi...@leica-microsystems.com, for product management support or Karen Niewerth,

Re: [Histonet] Could you please recommend? (Bascaramurty, Saro)

Not sure about the UV thingy but I love the Leica 1850 or the 1510 works great. But, if I had to choose between the two I would go with the Leica 1850.. You can purchased a used cryo for about 12-15,000. Most times with a one year warrenty. I recommend calling Margaret at IMEB for a quote. She

Re: [Histonet] microtome blade donations

Do they take volunteer histos, :) I would love to do somethig like that. Nicole Hi, One of our pathologists is going on a volunteer mission to a pathology lab in Malawi, Africa in a few weeks, and he is looking for donations of low profile microtome blades for the histology lab there.

[Histonet] New Position Alert - Field Histology Tech Southern CA

Good Afternoon Histonet, One of our retained Cancer Diagnostics clients has opened up an Field Technical Support Opportunity based in Southern CA. This position is perfect for a Histotech looking to get out of the lab and into the field. Field Support Specialist - IHC The primary

[Histonet] FW: The results of your email commands

Hi Histoland, Just wanted to give a quick update to a post I replied to a few weeks ago regarding floater contamination and it's relation to the Peloris processor. We've worked with Leica (thanks Cindy) and to reiterate what I stated in my response our source of contamination was most likely

[Histonet] Plastic processing for undecalcified mouse bones

Hello, All, I am looking into starting a plastic embedding/sectioning endeavor, and am trying to figure out my startup reagents and protocols. I am seeing a lot of protocols for the Technovit system, which uses a slide press to adhere the sections to the slides, and would prefer to avoid

[Histonet] Could you please recommend? Cryostats.

Hi, I am also in research, and as such I am at the bottom of the barrel when it comes to NEW equipment. (Yes even lower than normal histo labs, think on that!) I congratulate you on being able to wrangle new equipment out of management. Due to this I can't recommend specific equipment, but I

Re: [Histonet] Could you please recommend?

I have in my lab crytome FSE from shadon and it is working pretty well since last 5 years without any complain. Though i haven't experienced personally, but i heard this is better than leica one. also it has fumigation feature. Amita From: Bascaramurty, Saro