[Histonet] RE: Specimen Collection Instructions

2011-07-08 Thread Wanda.Smith
Mike, Pathology and Lab print a collection manual every few years with the requirements for blood test (tubes, amounts, etc) and collection requirements for cytology and tissue specimens (fresh, frozen sections, fresh fluids). We also have the manual on-line and we give the physician offices

[Histonet] safe handling of tissue for histology containing radionuclides

2011-07-08 Thread shehnaz khan
Could someone please shed some light. Hi Everyone, Could someone kindly shed some light on safe handling of tissue for histology containing radionuclides - to comply with CAP standards. How is this done? What should I include in the policy / work instruction? Thanks in advance S Kahn

Re: [Histonet] low profile vs high profile blades

2011-07-08 Thread Rene J Buesa
It depends on the embedded tissue. For harder tissues high profile are more suitable. René J. --- On Thu, 7/7/11, Liette Tougas ltou...@dawsoncollege.qc.ca wrote: From: Liette Tougas ltou...@dawsoncollege.qc.ca Subject: [Histonet] low profile vs high profile blades To:

Re: [Histonet] microtome knife holder

2011-07-08 Thread Emily Sours
All of the Leica cryostats have knife holders, I would contact Leica to ask as their website is terrible. You could also just google it and I bet some other company sells them. The other one, I'm not sure of. We don't use disposable blades so we've never had a knife holder for that. Emily A

Re: [Histonet] microtome knife holder

2011-07-08 Thread Rene J Buesa
Knives (not disposable blades) do not need holders. They are used by themselves. René J. --- On Thu, 7/7/11, Liette Tougas ltou...@dawsoncollege.qc.ca wrote: From: Liette Tougas ltou...@dawsoncollege.qc.ca Subject: [Histonet] microtome knife holder To: histonet@lists.utsouthwestern.edu

RE: [Histonet] safe handling of tissue for histology containingradionuclides

2011-07-08 Thread Connolly, Brett M
My lab work with tissues containing radionuclides fairly often, but since we are in industry and not under CAP regs I can only give you some general info. It depends on the specific radionuclide and thus it's half-life. Half-lifes for common SPECT isotopes are: I-125 60 days Tc-99m

RE: [Histonet] microtome knife holder

2011-07-08 Thread Connolly, Brett M
Um, actually steel knives do in fact require a holder and disposable blades require a different holder, we have both from Leica. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803

[Histonet] HM525 Cryostat

2011-07-08 Thread Nails, Felton
I purchased a new Microm HM525 cryostat about 3 months ago. The unit overheated causing some of the internal parts to warp. Has anyone else experienced this or similar issue? __ CONFIDENTIALITY NOTICE: The information in this

[Histonet] Embedding Media

2011-07-08 Thread Paula Lucas
Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on

[Histonet] Embedding Media

2011-07-08 Thread Elaine
We use Paraplast Plus. It's a little 'sticky but works very well. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Embedding Media

2011-07-08 Thread Tim Higgins
Hi Paula, Obviously there are no compression free paraffin's on the market. Look for a paraffin with a higher polymer content, this makes the paraffin firmer and less likely to compress. Other factor for into section compression but paraffin is a good place to start. The higher polymer

[Histonet] Direct Mouse IgG

2011-07-08 Thread Ross Benik
Hello Histonet, Does anyone have a working protocol that stains directly for mouse IgG (fluorescent) on frozen sections? We are currently staining with a goat anti mouse IgG 555 (highly cross absorbed) and we have having a hard time deciding what is specific staining and what is no.

[Histonet] HT Supervisor Position in Oklahoma City OK

2011-07-08 Thread Gaiser, Marcia
POSITION SUMMARY: Plan, coordinate, and supervise the functions and activities of personnel in the histology section of the pathology laboratory. Ensure the validity and accuracy of the test results. POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION,

[Histonet] Tissue controls

2011-07-08 Thread Tim Higgins
I have an abundance of H-pylori controls that I would be willing to part with and a number others as well. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histonet] Tissue controls

2011-07-08 Thread Elaine
Would you happen to have any AFB, fungus or copper controls?? Elaine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Embedding Media

2011-07-08 Thread histot...@imagesbyhopper.com
We use the infiltration embedding paraffin from Surgipath/Leica and are very happy with it. Michelle Sent from my iPhone On Jul 8, 2011, at 12:44 PM, Paula Lucas plu...@biopath.org wrote: Hello, We are considering a switch to a different brand of paraffin and this is because I feel

RE: [Histonet] Histonet] Tissue controls

2011-07-08 Thread Elaine
Well rotting meat is a new one for me!! We do purchase our AFB from them, we just do so many it would be more cost effective to have our own blocks. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu