[Histonet] Recycled alcohol

2011-07-22 Thread histot...@imagesbyhopper.com
Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic HE stainers? Are you having any troubles with proper, consistent HE staining? We are using recycled alcohol, and wish to continue to do so IF we can. We are having issues with our

[Histonet] Job Opening

2011-07-22 Thread Howery, Jeffrey
We are currently looking to fill a position here at Yavapai Regional Medical Center. please check out our website at YRMC.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged

[Histonet] BENCCHMARK LT

2011-07-22 Thread Sara Baldwin/mhhcc.org
HISTONETTERS Has anyone pulled the drawer out on the benchmark while the machine is on with the pressure on? I know there is a fix but the customer service has not caalled us back!! Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center

[Histonet] BENCHMARK LT

2011-07-22 Thread Sara Baldwin/mhhcc.org
THANKS EVERYONE GOT IT GOING AGAIN APPRECIATE YOUR HELP Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use

Re: [Histonet] Recycled alcohol

2011-07-22 Thread Rene J Buesa
Issues with recycled ethanol and xylene appear frequently in this list and I always answer the same: IF the recycling unit is working adequately and is maintained well to assure a final (recycled) product of good quality there should be no differences because ethanol and xylene will be pure.

Re: [Histonet] EDTA 14%

2011-07-22 Thread Rene J Buesa
Why don't you prepare it in your lab? By doing so you will cut costs and assure a consistent decal solution. René J. --- On Fri, 7/22/11, pam plumlee paw...@yahoo.com wrote: From: pam plumlee paw...@yahoo.com Subject: [Histonet] EDTA 14% To: histonet@lists.utsouthwestern.edu

[Histonet] Histotech Needed in PA

2011-07-22 Thread Alyssa Peterson
*Allied Search Partners is currently looking for a qualified Histotech for a laboratory in Lancaster, PA. We have a temporary or permanent position available. We are looking for local candidates only for the temp position, and the position would be effective August 1st. We are also looking for a

[Histonet] Re: 14% EDTA

2011-07-22 Thread gayle callis
There is no vendor I know of who sells 14% EDTA. It is important to remember that the EDTA you have to use is Tetrasodium EDTA, MW 452.2. This particular EDTA is highly soluble in water compared to EDTA without any sodiums, or disodium EDTA. Also, once dissolved it has a very high pH of

[Histonet] NBF waste disposal

2011-07-22 Thread Campbell, Sharon
Happy Friday everyone, I am currently researching ways to dispose of NBF waste. I would like to know if you are neutralizing the waste and then putting it down the drain or if you are shipping it off to be handled by another company. I am looking for the best and most cost effective way to

[Histonet] Re: Recycled alcohol

2011-07-22 Thread Tim Higgins
Hey Michelle, The easiest way to find out if it's the recycled regeants is to use fresh reagents and see how the stain looks. Obvisously if it still shows sign of a lack of color or detail than I would think it could be the stains themselves. What manufacture and type of Hematoxylin and Eosin

Re: [Histonet] NBF waste disposal

2011-07-22 Thread histot...@imagesbyhopper.com
Sharon, We use a product called Aldex to neutralize our formalin. It binds with the aldehyde groups and neutralizes them. According to the mfr, you can dispose of it in regular trash, but I am more cautious and throw it away in red bag trash. Michelle Sent from my iPhone On Jul 22, 2011, at

[Histonet] NBF disposal

2011-07-22 Thread Sara Baldwin/mhhcc.org
I am in Indiana we got inspected IDEM and they said even if you neutralize the formalin it is not suppose to be put down the drain, none the less we now have this hauled off. We also cannot put ETOH down the drain as well. Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial

Re: [Histonet] NBF waste disposal

2011-07-22 Thread Rene J Buesa
I have tried ALL available neutralizing agents in the market and ALL of them test positive for formalin after the neutralization is completed. I would not dump any neutralized formalin down the drain. After all those tests I ended returning to my reliable method of having someone to haul it out

[Histonet] RE: NBF waste disposal

2011-07-22 Thread Connolly, Brett M
Sharon, IMO - in today's world no laboratory waste should go down the drain. It is just not an environmentally responsible practice - and it all ends up eventually be discharged into in a stream somewhere. We collect all liquid waste (formalin, ETOH, xylenes, acids, bases, stains,

[Histonet] Paraffin Wax Waste Disposal

2011-07-22 Thread Joanne Clark
Hi All, we had our CAP inspection yesterday and were cited for disposing of our waste wax from the processors in regular waste. In all my 20+ years of working in histology I have never disposed of the dirty wax in biohazard waste. Especially now with the newer processors that have very little

[Histonet] Re: RPM on Cytocentrifuge for CSF

2011-07-22 Thread Matthew Lunetta
We have ours set at 750 RPM for 2 Min. Matt Lunetta BS,HT(ASCP) Longmont United Hospital -- Message: 2 Date: Thu, 21 Jul 2011 18:30:44 + From: Marcia Fisher Subject: [Histonet] RPM on Cytocentrifuge for CSF To: histonet@lists.utsouthwestern.edu Message-ID:

Re: [Histonet] Paraffin Wax Waste Disposal

2011-07-22 Thread Mark Tarango
That's funny that you got cited for that. I was surprised to learn what our safety officer setup at my lab for paraffin disposal. A commercial company takes our paraffin and makes a product with it that is mixed with cedar sawdust and paraffin wax for starting fires in the BBQ, fireplace, or

RE: [Histonet] Paraffin Wax Waste Disposal

2011-07-22 Thread Sherwood, Margaret
We have to have our processor paraffin picked up as hazardous waste, since it has traces of CitriSolv in it. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice

[Histonet] Tissue Processing: Blue Dye used to see small tissue??

2011-07-22 Thread Kiranjit Grewal
Hi,   Please let me know the name and ordering information of the blue dye used during processing. Currently we use eosin in all our alcohols but still tissues are very pale and hard to see at embedding.   Please share If anyone has any other sugesstions that will help to see small tissues

[Histonet] RE: Histonet Digest, Vol 92, Issue 29

2011-07-22 Thread Anastasia Pinkston
Kevin, This reply has nothing to do with your post. But my family and I will be moving to Tallahassee Florida soon and I would really like to speak with regrading Histology in that area. Would you please email me at anastasiapinks...@hotmail.com at your convenience? Ana Pinkston Greetings,