[Histonet] Full Time HT Position in Oklahoma City

POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) – or – other nationally recognized certifying agency acceptable to the Laboratory Director – or –

[Histonet] Cresyl violet stain on 50 um mouse brain sections

I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then

[Histonet] RE: Cresyl violet stain on 50 um mouse brain sections

Hi Susan, I would suggest cutting lots thinner sections. The plus slides seem to have trouble holding on to very thick sections. Another possibility is too much glacial acetic acid in your working solution of cresyl-too much acid can loosen the bonds on the plus slides. Tom T -Original

[Histonet] Re: 10% Buffered Formalin Acetate PBS precipitation artifacts

Hi all, This discussion of phosphate buffered formalin has raised some other questions for me. In paraffin embedding protocols for early mouse embryos, I have seen recommendations that dehydration begin with steps in 30% and 50% EtOH in 0.9% NaCl or PBS, then the 70% and higher dilutions are in

[Histonet] RE: Cresyl violet stain on 50 um mouse brain sections

Susan, If you are talking about frozen sections of fixed tissue, they do not adhere well to slides (even charged slides) no matter how thick or thin. I'm not sure why you are treating the sections with alcohol chloroform either. That is pretty harsh treatment and totally unnecessary for Nissl

[Histonet] (no subject)

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[Histonet] Cam 5.2

Thanks to everyone for the Cam5.2 info. A great weekend to all. Cindy Cindy Pyse, CLT, HT (ASCP) Laobratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Cresyl violet stain on 50 um mouse brain sections

We have done the same, but also adding a 50 and 70 ETOH as well. Our slides are also gel coated. We also have them in a 1 to 1 alcohol/chloroform, but only have them in for 30 minutes. Laura -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Immunotech

Does anyone know if Immunotech (France) is still around and, if so, do they have a distributor here in the US? We obtained an antibody to CD1a (clone O10) from them years ago through a distributor in Maine whose telephone number is no longer active. Thank you. Richard Richard W. Cartun, MS,

[Histonet] Sunquest CoPath

We are looking at the Vantage system and how it relates with Sunquest CoPath. Please share with me concerns or issues if you are using these systems.Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907

Re: [Histonet] cam 5.2

I have used CK-CAM5.2 for years with excellent results. However, for the past several years I have been using Leica-Microsystems' low molecular weight CK antibody, clone 5D3, that labels cytokeratins 8 and 18. I highly recommend it. Richard Richard W. Cartun, MS, PhD Director, Histology

RE: [Histonet] Cresyl violet stain on 50 um mouse brain sections

Susan, I have stained 60um fixed sections on plus slides without any problem. I free float the sections in a multi-well plate containing PBS or TBS. When mounting the sections onto the plus slide, I wipe away the excess fluid after EACH section, so that the previous section will not be exposed to

Re: [Histonet] Immunotech

Hi richard. I think immunotech was acquired by the now Beckmann coulter group. Tom Sent from my iPad On Jul 29, 2011, at 11:37 AM, Richard Cartun rcar...@harthosp.org wrote: Does anyone know if Immunotech (France) is still around and, if so, do they have a distributor here in the US? We

[Histonet] Slide/cassette printers

Is anyone having problems with the Thermo Printmate/ Slidemate and associated Cognex scanners? If so, what? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 250 Miller Place Hicksville, NY 11801 (631)435-1515x1018 This message contains privileged and confidential

[Histonet] CD117 - FDA APPROVED PROTOCOL

We are finding that the FDA approved protocol from Ventana does not stain some definite gi stromal tumors that stain quite well with another vendor's clone and protocol. My pathologists are trying to decide what to do with this. I would appreciate in hearing what kind of experience other

Re: [Histonet] CD117 - FDA APPROVED PROTOCOL

We're sticking to our IVD CD117/c-kit rabbit poly from Dako. Mark On Fri, Jul 29, 2011 at 10:42 AM, Vickroy, Jim vickroy@mhsil.comwrote: We are finding that the FDA approved protocol from Ventana does not stain some definite gi stromal tumors that stain quite well with another vendor's

[Histonet] RNAscope ISH technology

Happy Friday!   Does anyone have experience using RNAscope ISH technology on FFPE tissues? http://www.acdbio.com/whats_rnascope.html   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] molecular path email listserv

Hi Everyone,   Does anyone know of an email listserv similar to this one for molecular pathology?   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] ThinPrep

Does anyone know if the ThinPrep system has a reagent rental program available, rather than just purchasing the instrument? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] service on the Sukura Prisma

Hello, Does anyone know of an alternate vendor besides Sakura who can do the PM's and any necessary adjustments/fixes that come up on the Prisma? Sakura bills an arm and a leg for someone to come out. $175 dollars just for travel expenses is gouging to me, so if we can find someone else who

[Histonet] Histology lab assistant in CA

Hello everyone, I have a wonderful histo lab assistant who is moving from Oregon to the Lathrop, CA area. We absolutely hate to see her go, but must. If there is anyone with an assistant position open in that area would you please let me know so I can pass the info on to her. She would be a

[Histonet] Goeff's bottle top issue...

One of our aids was so sweet-she would loosen the lids as she accessioned -just enough it didn't take a vise-grip to open eash one. But that is just transferring the problem. I've used the tacky rubber grippers on some types of jars: put the jar on one on the counter for traction, put another