I have heard confliciting opinions.
Do Alcohol require an expiration date? Does anyone have a link to the regs on
this
Thanks
Dave Johnson
Mercedes Medical
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Histonet@lists.utsouthwestern.edu
I thought this email was about dating so you can get alcohol.as in
dating..LOL!
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi all,
I have a new employee who is developing a scratchy, painful throat and some
difficulty breathing when exposed to xylene (for instance, when the cover is
raised on the coverslipper). This is her first job in a lab of any sort. We
are investigating all the usual culprits--air handling
Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having
a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the
pain.
Your long term and complete solution is to eliminate xylene from your lab and I
Rene,
I understand the desire to remove Xylene from the lab completely. But what if
you are running FDA approved kits that are only FDA approved if the tissue is
processed in Xylene?
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical
Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation
time and NBF is required by some kit FDA approved, but not to how the tissues
are processed.
Even there are studies that show that xylene has extracting effects over some
epitopes.
I know that you cannot argue
Does anyone use the p57 (Ab3) concentrated antibody from Lab Vision (Fisher)
for Molar pregnancies on the Ventana platform using the UltraView detection
system? What is the protocol and dilution you run?
Any help would be greatly appreciated!!
Thanks,
Timothy Higgins, HT(ASCP), QIHC
The Hercept Test from Dako specifies xylene in the breast tissue processing
section. The Er/Pr Pharm DX kit may also, but I haven't check that out. I
know this because we just fought with a sister hospital to get them to change
their processors to xylene.
Loralee McMahon, HTL (ASCP)
Shelley,
Perhaps this employee should look into other job opportunities. The histo lab
is filled with chemicals that can cause sensitivity in those people who may be
prone to that. Unfortunately, some of the main players in the histo lab
(xylene formalin) are often the culprit, but they
Hello everyone:
I am Hortensia from the University of Valencia, from Spain. I am new in
this world. And I have some issues. I will apreciatte if someone with
experience can help me.
I am working with endometrial tissue samples, from biopses and
endometrial aspirates. I want to detect the
Hello Histonetters,
Just drooping in to say thanks to all those Who have joined Histo-ville on
facebook. We are forming an awesome community and hope more will join.
Stop by and like us.
Nicole Tatum, HT ASCP
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Loralee:
Poor people in your sister hospital! You are affecting their health. I would
have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my
legal department to handle that.
I would have requested the experimental data
I have gone around and around with DAKO because they want to blame the fact
that I use Pro-Par rather than xylene in my lab. I use it for processing and
de-paraffinizing and some of their people want to blame that on their Artisan
Link Auto Stainer staining inconsistently in my lab. . . I like
My best friend just lost a job not too long ago because she was accused of NOT
telling the lady she interviewed with that she had a sensitivity to xylene! I
know she had to have told her because she hasn't worked with xylene in 20
years!
Kelly
-Original Message-
From:
Shelly,
Is your new employee a certified histotech? Did she rotate through
clinical labs while in histo school? I would think she would have
experienced this sensitivity during that time period as well.
Tina
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Rene',
I must respectfully ask: what happens after you modify your procedures for this
new employee 2 days after everything has been standardized/validated another
employee begins employment, but exhibits sensitivity to the new xylene
substitute? I realize that getting another job NOW is
Rene
Rene
Loralee is correct in some aspects and you have addressed this already with
mentioning validation. This basically has to do with the internal validation
that is required when you bring these kits into the laboratory. When you
modify a 510K cleared kit or FDA approved kit it
Glen:
Unfortunately for your reasoning, I substituted xylene with MINERAL OIL, the
same mineral oil you can profusely use on your skin while applying a sun
blocker, or the same mineral oil that constitutes the baby oil sold in any
drug store to be applied to the any baby's hypersensitive skin.
Glen,
I suppose it would all depend upon which xylene substitute one uses - if one
switches to another that has a pungent odor such as the citrus based solvents -
there could be problems with that as well. I have no scientific data to back
this up nor have I researched it, but I would think if
The subject of the odor itself is interesting. I had a colleague years ago
that tried Pro-par and couldn't use it because she said it smelled like bug
spray and it just made her nauseous. I noticed the odor but it didn't offend
me nearly as much as it did her.
Jeanine Bartlett
Infectious
Sometimes a new employee is like the old canary miners took with them. They
alert you to bad air. Sometimes, after you have used a product for a while your
body has adjusted to it and you cease to notice it.
We use xylene, the last time a substitute was looked at one of the techs had a
Well actually yes. We do a trend analysis and have proven that according to
the accepted rates their Her2 positivity was declining (went from 14%- 6%).
Meaning that some patients did not get the correct therapy. I would have to
say poor patient!!
And maybe it wasn't the xylene but if I was
Some points that haven't been mentioned in this discussion:
I think most labs have switched from xylenes to aliphatic hydrocarbons
for processing, hydration, and dehydration though obviously
considerable controversy remains. (I can't imagine anybody switching
from xylene to limonene now -
Hi Histonetters,
We would like to start to run up to 30 H.Pylori on our Benchmark daily. Do GI
labs that run H.P. on ALL stomach biopsies run a negative control for each
patient tissue for H.P.? I am just curious, as we have a 24 hour TAT, but the
Benchmark only holds 30 slides. What's
https://www.facebook.com/#!/pages/Histo-Ville/114622425272649
Here is the link for those of you who need it.
Hello Histonetters,
Just drooping in to say thanks to all those Who have joined Histo-ville on
facebook. We are forming an awesome community and hope more will join.
Stop by and
Liz,
You are correct. If you bring in an FDA-approved test with NO
modifications, all that is required is verification; you simply have to
verify that the test works in your lab when you follow the recommended
procedure. Validation, which is required for laboratory-developed tests
as well as
Hi histonetters,
I'm conducting a small benchmark study regarding workload distribution and
times slides get to pathologists, etc. We are NJ healthsystem with 2
hospitals but only do histo in one. We do 14k cases/16k blocks/year, 500
IHC/month and some special stains.
If you are a hospital or
You are assuming that the decline in your sister lab was due to not using
xylene and you should never assume.
I would have taken several cases of mine, divided in two parts and given your
sister lab half, and viceversa with some of their cases.
Compare both results and try to determine what was
Kathryn,
Thanks for the advice. I will evaluate the installation of a hood.
Regards,
Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas
-Original Message-
From: Stoll, Kathryn
We did not assume anything.
My sister hospital does not perform any immunos, we do all the immunos for
them. So that means the only variable is the processing of the tissue. So
where would you have started..?
Renee I really take offense to the fact that you just told me that I don't take
We are looking for a certified HT or HTL, or applicants eligible to sit for the
certification exam, to join our pathology lab in beautiful, downtown,
Manitowoc, WI. We currently have a fantastic 2nd shift position open. We
have excellent benefits ( 13% employer money contributed to
Hi Sarah,
Thanks so much for the information. We do have a small fume extractor next to
our formalin containers in the Gross Room. This might be worth a try for the
coverslipper anyway. I believe that the piece of equipment I am thinking of
buying while we evaluate xylene replacements is a
Under separate cover I am sending you an article I wrote on the subject.
Just find in the benchmarks the column corresponding to the lab class you
belong to and you will find the information you need.
René J.
--- On Tue, 9/27/11, andrea conard andrea.con...@gmail.com wrote:
From: andrea conard
Does anyone know of resources for find accurate wage information for histology
personnel in a clinic setting (particularly in Wisconsin)?
Thanks, any help would be greatly appreciated.
Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
Fax: (920)663-9004
I agree. Sorry...
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: Sarah Dysart [sdys...@mirnarx.com]
Sent: Tuesday, September 27, 2011 4:10 PM
To: McMahon,
Hello everyone
Does anyone out there know if you can purchase this reagent, from my searches
it looks like a modified Schiff reagent. When someone is requesting this stain
as the Feulgan and Rossenbeck is it just the Feulgan stain? Sorry this
question seems really basic but to be honest I'm
Hi Tom,
Yes, our Employee Health Safety department is involved with this problem.
Our lab is negative pressure, but do not know the air exchange rate. I will
definitely ask that to be checked.
We monitor employees annually and had a full environmental assessment 2-3 years
ago prior to
Hi Jan,
Thanks for the advice on xylene substitutes. Your symptoms mirror those of my
affected employee and I'm glad to hear that you found relief using another
product. I'm anxious to fix this situation as quickly as possible. This
employee is ready to make a career of Histology and I would
Rene',
Thanks for the quick response to my question. The two articles that you sent
were very interesting and I have forwarded them to my department histotech
supervisor for her evaluation.
It is apparent from your comments on the listserv that you have a strong
commitment to safety. You
Hi,
I can attest to the effacacy of the isopropyl/mineral oil processing
that Rene describes. It works great for murine heart samples. I would not
switch entirely though, since xylene does a fine job with most other
purposes. My main concern is the rather hysterical impulse to get rid of
every
I received a call from Mercedes Medical saying they can not get the KP
Markers so many of us like. Does any one have another source for these
wonderful pens? Thanks and have a wonderful evening, Kathy Gorham H.T.
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by
From my experience visiting Teri's lab I can attest that the Stower's
institute is an astounding institution and a fantastic opportunity. Anyone
interested in working in a truly top notch research environment that values
its people should take her up on it immediately.
Tim Morken
Supervisor,
I received the same info. If someone has another source, please let us
all know.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Tuesday, September 27, 2011 2:18 PM
To:
Got to StainsFile at for details of Feulgen and Rossenbeck;s Schiff
reagent.
http://stainsfile.info/StainsFile/stain/schiff/feulgenrossenbeck.htm
Bryan Llewellyn
Elizabeth Chlipala wrote:
Hello everyone
Does anyone out there know if you can purchase this reagent, from my searches
it
Have you already consulted the annual survey conducted by the ASCP? It is
available on their website, and divides salaries by title, geographic region
and facility size.
Joelle
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: Michael Hillmer mhill...@dermwisconsin.com
Thanks Steve,
Now I don't feel as if I am trying to slowly kill my technician!!
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
I understand the reluctance to discontinue the use of the xylene in the
tissue processor for FDA compliance, but why not change the solvent for
staining and coverslipping? Just curious.
Jennifer MacDonald
D'Attilio, Shelley sdatt...@stormontvail.org
Sent by:
Hi All,
Have someone a good workong protocol for staining mastcells in cytospin slides
with Toluidine Blue.
Mention also the pH and fixation.
Thanks,
Henk van de Kant
Utrecht University, Faculty of Science, Division of Pharmacology,
David de Wied Building, Universiteitsweg 99, 3584 CG, Utrecht
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