[Histonet] Anti- guinea pig polymer secondary

Hi all, Anyone aware of any anti-guinea pig secondary HRP polymer detection kits. I found one from Golden Bridge Int., Inc. and am asking if anyone has used it. Any other vendors making this?? Thanks, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck Co., Inc. PO Box 4, WP-44K

RE: [Histonet] Anti- guinea pig polymer secondary

Brett I just use a rabbit anti gp link and then follow with rab labeled polymer HRP, I get links from Southern Biotech. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original

[Histonet] RE: Histonet Digest, Vol 95, Issue 31

Hi Kevin, I currently have the Leica IPS and IPC labeling system. There are some definitely pros/cons: Pros: Great for high volume Has convenient tray loading attachment (optional) Cons: Takes up a lot of space Very particular for type of slides used (may be a issue for cost saving purposes)

[Histonet] Microm HM 560 vacutome for brain sections?

Hello Everyone, I'm thinking about purchasing a cryostat. I will most likely section primarily rodent brains and I'm concerned about wrinkling. One rep suggested the vacutome system, which according to the literature the patented vacutome stretches sections to eradicate artifacts. I was also

[Histonet] Stripping antibodies

Hi! Does anyone have experience in IHC stripping antibodies from a tissue section and re-probing with different antibodies? What is the best way to do that? If you could share your stripping protocol or experience I would appreciate it - thanks! Claire Claire Weston, PhD Research

Re: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment

Why would you like to avoid detection of intracellular Abeta inclusions? //Daniel On Wed, Oct 26, 2011 at 8:51 AM, Hobbs, Carl carl.ho...@kcl.ac.uk wrote: Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ;-))

[Histonet] used cryostat opinion

Hi Guys, I'm looking to section rat brain. A cryostat is not a huge necessity for my lab, I can borrow access from another. But it would be a nice item to have and I'm considering a few refurbished models, a Leica CM1800, CM1850 and CM1900. The difference in price between the 1800 and 1900 is

[Histonet] oops

Hi all, Bad day here. Left the IHC stainer to attend a meeting and the volume of the DAB was not sufficient to stain all slides. By the time I realized this, they had been counterstained. I immediately applied DAB to the affected slides but naturally the antigen is not abundant if present

Re: [Histonet] oops

Oops. Yes hematoxylin staining interferes with DAB staining. Sorry! You can save the slides by re-retrieving and restaining. Will Sent from my iPhone On Oct 27, 2011, at 10:59 AM, Denise G Crowley dencr...@mit.edu wrote: Hi all, Bad day here. Left the IHC stainer to attend a meeting

[Histonet] CPT CODE

Does anyone know the CPT code for Anal pap smear THANK YOU,   PATTI RUBEN-NELSON  H.T.(ASCP) nelsonr...@verizon.net     CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants   and are intended only for the addressee.  The

RE: [Histonet] CPT CODE

Patti, We use CPT code 88112 - Cytopathology, selective cellular enhancement technique with interpretation (e.g. liquid based slide preparation method) except cervical or vaginal. (CPT 88112) Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT

RE: [Histonet] oops

I have done cs before DAB lots of times with no problems, the only time I would expect a problem would be if your antigen is expressed in the nuclei and the cs is too dark. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110

RE: [Histonet] Abs for detecting immune cells in mouse FFPE sections

These are two good databases for antibodies and protocols for murine IHC: From the pathology lab at The Jackson Lab: http://tumor.informatics.jax.org/html/antibodies.html From the animal pathology lab at NCI: http://web.ncifcrf.gov/rtp/lasp/phl/immuno/ Best, Sebastien Sébastien Monette,

[Histonet] UAMS Little Rock AR

Good Morning, We are looking for an ASCP registered Histologist for a fulltime position at the University of Arkansas for Medical Sciences in Little Rock. We have an opening for an early morning shift person who is HT registered with clinical experience. We have great equipment with more

RE: [Histonet] CPT CODE

Shane, It depends on the prep you use. If it is a smear it should be 88104, if a thin prep 88312. Jan Omaha Date: Thu, 27 Oct 2011 11:29:25 -0700 From: nelsonr...@verizon.net To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] CPT CODE Does anyone know the CPT code for

RE: [Histonet] CPT CODE

Sorry, non-gyn thin prep, 88112. Jan Date: Thu, 27 Oct 2011 11:29:25 -0700 From: nelsonr...@verizon.net To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] CPT CODE Does anyone know the CPT code for Anal pap smear THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP)

[Histonet] Fellow Dermpath Lab Managers

Hello All! Wondering if anyone would be willing to email me privately to discuss salaries for Dermpath Lab Managers.  My docs have been getting info from a lot of other docs out there as to what they pay their Lab Managers and I was hoping to do the same. If anyone has any other ideas on how

[Histonet] Carmine

Thanks, Peggy for drawing attention to the Perfect Red book. Must check it out! Carmine in histology wasn't always just mucicarmine. For many years carmine was the only stain. Corti used it to find the sensory organ for hearing in the cochlea (the dye was an aid to microdissection), and