With quantum dots and a modern fluorescence microscope with "channels" it
should be possible to label 2 or 3 antigens simultaneously, if you have all the
right primaries and labelled secondaries. The company that sells you the
quantum dot labelled secondaries or other amplification reagents shou
Thank you
everyone for your advice, I really appreciate it! I am doing a multiplex
immunofluorescent assay (4 channels) and I am planning to strip the antibodies
and reprobe with a second round of antibodies. The antibodies are
labelled with quantum dots so I am not sure how that will influen
Hi,
My respects! I wanted to do some zebrafish larvae sectioning. But some slice I
made showed that the tissue inside had been broken. As to me, I thought the
problem might be in dehydration time and and the razor I used. So can you
please give me some advice to improve the process? In addition
Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes
enzyme-antibody conjugates. The coloured products of the enzyme histochemical
reactions are generally not affected. The brown oxidation product of DAB (from
peroxidase) and the blue products from bromochloroindoxyl/t
