With quantum dots and a modern fluorescence microscope with channels it
should be possible to label 2 or 3 antigens simultaneously, if you have all the
right primaries and labelled secondaries. The company that sells you the
quantum dot labelled secondaries or other amplification reagents
Histotechnologists are the most valuable employees in the laboratory
today!
THANK YOU DR. CARTUN!!
Nancy Heath, HT (ASCP)
Neuropathology Technician
Neuropathology Department
Rhode Island Hospital
President - Rhode Island Society for Histotechnology
-Original Message-
From:
Just got offered a lead tech position, salary of $85600.00 in a private lab
opened by a group of docs. Didn't take it because the health insurance plan
they offered was lousey. 75% health covered with a 250.00 deductable, dental
covered at 60% and no coverage for family or husbandwould of
I agree :)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Friday, October 28, 2011 3:22 PM
To: Podawiltz, Thomas; Richard Cartun; kcasti...@frii.com;
Histonet@lists.utsouthwestern.edu;
That's a hoot Sally! I don't think that DST of old was even this past weekend.
For the past 3 years my processor changed on the DST of old and the powers
that be told me it was impossible because that wasn't programmed into my
processor's data. This year I forgot about it and it didn't
What are the true regulations' in Florida as far as time required for a
Medical Director to be on site at the lab ? I am finding conflicting
information and would like to know what is truly required. Thanks
Meredith Hale HT (ASCP)cm
Operations Liaision Director and Education Coordinator
I'm looking for some advice on frozen tissues. This is the first time I'm
doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut
well onto special Gold Plus slides from Fisher. Then, when I was ready to
stain the slides, i air dried them fro an hour and wanted to do HE and
Amos and John,
I do not agree with John saying that antibodies could be easily removed by
acidity pH1-2. High affinity primaries will stay at the tissue section nicely
bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual
dilution. After this incubation strip off the
Yes it was this past weekend back when. My alarm clock went to DST Sunday
morning.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Monday, October 31, 2011 7:40 AM
To: 'Breeden,
Add location to that. $28-32 is great for places that do not have an abundant
supply of techs. Here in Houston, we have 2 schools and a very large medical
center. Entry level techs are not hard to find. Graduates expect $23-28
minimum depending on facility.
In other states, neighboring
I know the cost of living is greater on the east coast, but that sounds good to
me. The insurance is not better, and as an HTL I make less than 1/2 of that!
Things are determined by the market I realize, but all these postings are sure
making me feel bad and also realize that I am not
Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply
gentle pressure to center of block and slide complex, cool to RT and repeat 1
or two times.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Still sounds good to me, at my first supervisor's job ( the credentials I had
at the time were HTL + Bachelor's), and I made less than $23 per hour and
worked an average of 16 hours a day with no OT or comp time that was ever
granted ( though promised). I guess I am just trying to say, count
I am currently filing out my ahca renewal application. There are many
changes and the form and I do not find it user friendly. Anyways here my
question: Medical directors and chief finicial officers or any person who
stands to gain profit must undergo a level 2 background screeening. On the
new
Hi Friends,
If anyone is using Dako PR concentrate clone 1294 on a Ventana, could you
please contact me separately?
I'd like some advice.
Thank you
Becky
Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771
-Original
Ive never just air dried my frozen sections. always put them in a fixative such
as pen fix, a alcohol and or formalin mixture, something( depends on what your
going to look for, test etc). That with using charged slides and never had too
many problems with this.
Kim
Hi,
Just curious because this questions has come up multiple times from
non-histology people so I thought I'd post it here. When labeling slides at
microtomy what info do you include on the slide? I know the automated slide
labelers that are out there will include patient name, surgical number
If your antibodies are labeled with quantum dots and you strip the
antibodies (e.g. with low pH) you will no longer have labeled targets in
your tissue. Not clear if you understood this from your post.
Message: 2
Date: Sun, 30 Oct 2011 11:58:19 -0700
From: Claire Weston
Thanks Chris. I didn't know there were antibodies with such strong adhesion.
John.
= = =
On 31/10/11, C.M. van der Loos c.m.vanderl...@amc.uva.nl wrote:
Amos and John,
I do not agree with John saying that antibodies could be easily removed by
acidity pH1-2. High affinity primaries will
Hi Lisa -
We hand write our labels, but do so prior to cutting sections.
The accession numbers [unique to every slide] are read from a printed data
sheet and written on the slides. This allows verification that the numbers
match [slide and block] as the tissues are cut. If the numbers are
Everyone employed in our hospital undergoes periodic background checks.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole
Tatum
Sent: Monday, October 31, 2011 9:33 AM
To:
November 4-5, 2011 is the SC Society of Histotechnology meeting at Marina Inn
at Grande Dunes in Myrtle Beach, SC. Everyone is still invited even if you
have not pre-registered! Gayle Callis is presenting 2 workshops and we
will have good food and good fellowship If you think you are
Hi all,
I wanted to ask if any of you out there are currently using an automated
coverslipper and do not have issues with the forks picking up the slides if
they have Ventana labels on them. I have used a Leica and had only very
occasionally a dropped slide. Is this your experience as well?
Hi Teri,
And welcome to San Diego :-)
My experience is, that you cannot leave the slides with the Ventana labels
sitting in xylene for too long before coverslipping. I believe it is just how
these labels are and doesn't have much to do with the coverslipper itself.
Bea
Beatrice
Good Afternoon,
New Position Alert!
Our client is a world leader in Cancer Diagnostics who is currently seeking
Histotechs who have experience working with IHC analyzers that would be
interested in Field Support roles. We currently have an opening covering
Northern FL, AL, and part of GA.
Not so good when you fall into a higher tax bracket and uncle sam takes
25% of your salary and the insurance with dental weekly would have cost
me around $232.55 a week...no thanks
From: joelle weaver [mailto:joellewea...@hotmail.com]
Sent: Monday, October 31, 2011 10:35 AM
To: Heath, Nancy
allison, we do all of our alcian blue on the machine. we love it!!
anita dudley
providence hosp
mobile, ala
From: allison_sc...@hchd.tmc.edu
To: histonet@lists.utsouthwestern.edu
Date: Fri, 21 Oct 2011 14:00:44 +
Subject: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus
We have a 15 year old Sakura film coverslipper that has performed famously with
Venatana labels.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
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