Re: [Histonet] b-cells stain

Thanks John, Since I was writing the message about Gomori's chrome alum hematoxylin without the procedure in front of me yesterday (not the best idea, I know) I didn't have the whole formulation available to me. For Gomori's chrome alum hematoxylin, in addition to potassium dichromate, there

[Histonet] UAMS Little Rock for HT or HTL

Good Morning, We are looking for a register Histologist for a fulltime job at the University of Arkansas for Medical Sciences in Little Rock.This is a chance to learn and grow with the Histology department at UAMS. The benefits are great and the retirement is a matching up to 10% for your

[Histonet] EM Processors

Looking for input from hospitals/institutions that process tissues for electron microscopy. I'm interested in what automated (such as Lynx) or microwave processors for EM are being utilized. What if any issues you have had and what you would recommended. Thanks for any and all feedback. Jean

[Histonet] HE stainers

We are in the market for a new HE stainer. We probably won't be doing any special stains with it. Does anyone use the Shur/stain automated slide stainer by TBS? If so what kind of repair record does it have? Are you satisfied with it? Thanks for any info you would like to share. Margaret

[Histonet] Microm STP120 and/or Linistain GLX-stainer

I was wonder if anyone in the histonet community users a Microm STP-120 processor and /or a Linistain Random access GLX-stainer. If so, I would very much like to hear from you. Thanks, Bret Clough Texas AM Health Science Center Temple,Texas ___

[Histonet] Xylene Recycler

Our lab currently has a CBG BioTech xylene recycler. We only recycle xylene on the instrument not alcohol. Today while collecting our alcohol waste (which is usually clear) our techs noticed that the entire solution is cloudy; after sitting it appears to have some type of white material

[Histonet] Movat's Pentachrome in ChicagoLand?

If anyone is running Movat's Pentachrome stain in the Chicago Area, could you please contact me? Thanks Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Legal Disclaimer: Information contained in this e-mail, including any files

[Histonet] Full time grossing tech wanted

Fast growing laboratory in the Boston area is seeking a full-time grossing tech with experience. Shift 4-10pm M-F, somewhat flexible. Must be ASCP/HTL certified. Please contact janto...@dnellc.com. ___ Histonet mailing list

[Histonet] Clostridium difficile - what do you do

Histonetters- We are about to embark on a project involving C.diff infected GI tissue. We have processes in place for PPE, decontamination/ disinfection etc... but I am looking for any evidence whether or not formalin fixation or subsequent tissue processing will inactivate the spores. Also,

[Histonet] Using methyl benzoate on a VIP 1000

I have a customer who would like to use methyl benzoate on a VIP 1000. Sakura says that it is not on the list of reagents so it is not recommended. Can anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue processors? Will this work? What are the problems? Thanks!

[Histonet] RE: Using methyl benzoate on a VIP 1000

not familiar with its use nowadays, but I remember it being combined with celloidin (methyl benzoate celloidin) as part of a double-embedding protocol used for autopsy tissues. Personally I have not heard of it being used alone. Can still remember floating in the room where we had all our open

Re: [Histonet] Using methyl benzoate on a VIP 1000

The problem could be that if something happens to the VIP Sakura will blame you and any guarantees will be forfeit and repairs cost will increase, especially after you asked them. René J. --- On Fri, 12/2/11, Kevin Seguin kevinseg...@gmi-inc.com wrote: From: Kevin Seguin

[Histonet] RE: Using methyl benzoate on a VIP 1000

This thing was used as an intermediate step in paraffin processing of some difficult tissues such as whole mouse eye or amphibian eggs between xylene and paraffin. Awful smell, but some people like it - benzyl benzoate smells the same and is used as food preservative. Root beer has a strong

RE: [Histonet] Chameleon tissue lifting off

Scott, Thank you for your recommendation of the Superfrost ultra plus slides. I have just contacted the company for a sample box. I received a few other suggestions too, and will keep that info for possible future use. I will keep your email address, as well and maybe I will be able to help

[Histonet] (no subject)

Ok Sent via BlackBerry by ATT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Rbt on Rbt

Hi Karen ( Histonet) Sorry for the long 'tutorial' here. In principle you can make your own rabbit on rabbit pre-treatment before adding your primary. The problem is specifically with any of the specimens's own IgG (rabbit, obviously) bound to your tissue. I haven't actually done it in rabbit

[Histonet] For HE staining - TBS SHUR/Stain or Leica Autostainer XL

I have and love my old Autostainer XL. Now we are offered a new TBS SHUR/Stain unit, which is attractively small, but I have never used nor seen it in person. We are a small University based lab and our space is limited. We rarely run few racks at a time. However, we must be able to run only

Re: [Histonet] Using methyl benzoate on a VIP 1000

Methyl benzoate smells really awful. Its only virtue is a high refractive index, which can make it useful for storing stained whole-mounts of small objects like chick embryos. Methyl salicylate (refractive index only slightly lower) is generally preferred because its wintergreen smell is quite