[Histonet] Stain recipe

All, Greetings from across the pond! One of our histology technicians is looking for a recipe for an alcian blue/sirius red stain, otherwise known as Alcian blue 8G with chlorantine fast red 5B. It is not one I have used but I suggested tapping into the wealth of knowledge on this forum - can

[Histonet] .

good day http://indianpastryhouse.com/index754m--.php?oxiprofileID=88 Tue, 13 Dec 2011 11:01:38 __ Were both going to work in the mill next Monday. (c) Kambrie wentelstok ___ Histonet mailing list

Re: [Histonet] gelatine, silane, super frost plus slides ???

I'll take a stab at it. All the subbed slides (short for submerged in a solution) have a coating that makes them for positively charged. The lab can make a solution to submerge the slides, or the vendor can submerge the slides and sell the slides pre-made. Or, the material can be placed in

RE: [Histonet] Stain recipe

Hi Catherine, I have staining protocol for alcian blue and nuclear fast red, see bellow. I looked in Conn's biological stains book (9th edition,p.188)and saw that chlorantine fast red 5B is soluble in water. I think you can substitute Nuclear fast red with chlorantine fast red 5B and try and

[Histonet] Stain and component shelf life

Can someone direct me to a reliable source for information on shelf life of stains and components, specifically expiration date for unopened vs. opened powdered/dry/granular stains? I have heard unopened dry stains w/shelf life of 10 years; opened dry stains w/shelf life of 5 years. Any citation

Re: [Histonet] Stain recipe

The method is found in Culling, Ed. 2., p.251. Solutions 1) Alcian blue Alcian blue 8G 1% aqueous 50 mL Acetic acid 1% aqueous 50 mL Filter, and add 10-20 mg thymol. 2) Phosphomolybdic acid, 1% aqueous 3) Chlorantine fast red 5B,

RE: [Histonet] Stain and component shelf life

Can you share this info with me too? We just got grief about this from our JC inspector about a month ago. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/13/2011 8:04 AM To: histonet@lists.utsouthwestern.edu

RE: [Histonet] Stain and component shelf life

I am still using some stains from late 1800 to yearly 1900 (azocarmine, pyronin, indigocarmine) from IG Furberindustrie - and they are better than contemporary from Sigma. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203

[Histonet] Stain Dye Stability: The Reference

I received an excellent reference relating to shelf life of dye and stain powders and would like to post this as a reference for anyone having questions. The journal BIOTECHNIC HISTOCHEMISTRY 2009, 84(1): 11-15. I believe that this reference could stand the Credibility Test for accrediting

Re: [Histonet] Histo Aide Duties

So reagent changes in the VIP or recycling would be out too? Kathy Boozer, HT (ASCP), IHCQ Histology Department, Lab 503-251-6266 ex 10246 Rene J Buesa rjbu...@yahoo.com 12/7/2011 7:54 AM Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the

[Histonet] Processor Help

If anyone has a Shandon Excelsior ES and can offer me some help, I would greatly appreciate it. I have one and have used it very little as it was the back up processor. My old work horse, Pathcentre, is down and now the Excelsior ES will be my main processor. I have zero training and zero

Re: [Histonet] Histo Aide Duties

Not in my lab. René J. --- On Tue, 12/13/11, Kathleen Boozer booze...@ah.org wrote: From: Kathleen Boozer booze...@ah.org Subject: Re: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-requ...@lists.utsouthwestern.edu, Matthew Lunetta mlune...@luhcares.org, Rene J

[Histonet] AUTO: is out of the office. (returning Fri 11/18/2011)

I am out of the office from Thu 11/17/2011 until Fri 02/10/2012. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mi...@leica-microsystems.com, for product management support or Karen Niewerth,

Re: [Histonet] Stain recipe

This is an instance where you must be careful not to use the wrong dye! Alcian blue 8G (CI 74240, Ingrain blue 1) should pose no problems. Use a certified batch. Chlorantine fast red 5B is also called sirius red 4B (CI 28160, Direct red 81). This dye, which has been used as a counterstain

[Histonet] DAB haematoxylin counterstain; too purple, overpowering IHC

Hi all, I have been working through an immuno using DAB and counterstaining with harris haematoxylin. Protocol for counterstain: 5 min h20 1 dip haematoxylin 1 min h20 2 dips Blue in ammonia 1 min h20 Dehydrate, clear, mount I am finding that my slides are coming out reay

RE: [Histonet] Stain recipe. Nuclear fast red

Sorry to make a blunt contradiction, because a few histonetters seem to take offence very easily. Nuclear fast red cannot replace any chlorantine dye. The chlorantine dyes in the literature and lore of staining are anionic; they bind to proteins such as those in cytoplasm, collagen etc.