John,
No offence, there is so much in histology I don't know, and any way I can learn
is good. I use your textbook all the time.
In this case I meant to use chlorantine fast red 5B, which they want to use,
instead of nuclear fast red, which is in my protocol.
What protocol would you suggest for
We are doing a Hale's colloidal iron stain (no counterstain) on serial sections
of mouse kidneys. We are staining an entire kidney at a time (about 90-150
slides), and after many successful runs, we are now finding some slides in each
batch with very uneven staining. Half of a section will
On 12/14/2011 9:55 AM, Elizabeth Cameron wrote:
We are doing a Hale's colloidal iron stain (no counterstain) on serial sections
of mouse kidneys. We are staining an entire kidney at a time (about 90-150
slides), and after many successful runs, we are now finding some slides in each
batch
I received a reply yesterday from an individual who attached a link to
the article in Biotechnic Histochemistry relating to stability of dry
stains. I have deleted that link but have had some inquiries from
others about that article. Would the person who posted in response to
my inquiry please
Please share the link with all. I have the reference you mentioned.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
On behalf of a friend, I am posting this part-time job request.
Houston Urology Partners in Houston Texas has a PRN position available. For
more details, please contact Linda Travitz at 713-861-9990 or via email at
ltrav...@hotmail.commailto:ltrav...@hotmail.com
Thank you
Candy Bales, HT
Chief
If the objects of interest are stained with alcian blue, any pink or red
counterstain should be OK. Aluminium-nuclear fasr red if they want to see
nuclei; eosin if a general pink to red background is sufficient. HE is also OK
after alcian blue because the two blue colours are clearly different.
Hi Histonetters!!
I hope everybody is having a great day. I have a new position to post
here on the histonet.
Here is the info:
Histology Supervisor - Nights for a Great Private Lab in Miami, FL
RELIA Solutions the nations only recruiting firm specializing in the
nationwide permanent
Ditto, to the previous messages my best stain powders are from the early
1900's.
For those CAP accredited lab, this is what CAP has to say about stains w/o
expiration dates (below)
What I have done to comply is, I have a policy that states that I evaluate
reagents at least annually
For those of you who are patiently waiting for a link to the article, I
am waiting for the person to re-post the link. If there is any way you
might be able to access the article by searching online, it comes from
Biotechnic Histochemistry 2009, 84(1): 11-15. Possibly there is some
sort of link
I will be out of the office starting 12/14/2011 and will not return until
12/15/2011.
Note: For Cytology issues, please call Molly at 8-421-5487, Eric at
8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call
Client services 8-421-5408 to reach Maria (IHC), Mario at
Hello,
We have an immediate need for a Histotechnician for our client in the East Bay
of San Francisco. Approximately 3 month assignment, day/early morning shift
available working in a hospital lab. Requirements: At least 6 months of paid
experience in a histology laboratory preparing and
Hello Histonet,
I hope you can help me with some troubleshooting. I am having some background
issues that I am not sure how to get rid of. I actually am working with two
different ChAT antibodies and trying to get at least one of them up and running.
I am performing IHC on FFPE Sheep sciatic
Dear Sara,
The Biological Stain Commission web site http://biostain.com has a Members
Only section that provides access to the complete archive of Biotechnic
Histochemistry and its predecessor, Stain Technology.
The publisher's web site http://informahealthcare.com (with links to find
Hi Megan,
Try to lift the coveslip (soak in xylene for a couple of hours), remove excess
of mountant in xylene and then go into alcohol. Dip your slides in alcohol plus
hydrochloric acid (95 ml alcohol absolute + 5 ml conc. HCl) for 5 min and then
into water. Most of the hematoxylin will be
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