Hi. I was wondering if anyone would be willing to share a copy of their
control block log. I'm creating a new one and want to see what others are
doing, for reference. I'm just looking for a blank one.
Thanks,
Steve Clark
Histology Supervisor
Grand Strand Regional Medical Center
To all:
I know this has come up before, but where do most people buy their (+) control
slides for special stains?
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
We get ours from Globe http://www.globescientific.com/ Their prices are
reasonable, and we have not had any problems with either ihc or special stains.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
I never bought a single (+) control slide. I prepared mine from the (+) cases
we had. I think you should try this avenue as well.
René J.
--- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: Re: [Histonet] Control
Same here René, unless we can absolutely not find a positive case.
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
How could you be sure those cases were positive if you didn't have a positive
control before, the eternal palindrome...
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 21 December
That is ideal Rene, but we are a research lab and don't run the same tissue all
the time.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983
Hi Everyone,
Does anyone know how close in proximity 2 proteins need to be for them to show
up as co-localized via immunofluorescence staining?
I am trying to compare routine 2-color IF vs. the Proximity Ligation Assay
(which will not show co-localization unless the proteins are withing 40
There was always a first positive that was used to evaluate the following and
so on...so on... etc
Besides, you know what to expect with a procedure and when you find it in some
tissue, you know it. Elemental dear Watson.
Merry Christmas
René J.
--- On Wed, 12/21/11, Essex, David
Hello,
We have an immediate need for a Histotechnician for our client in the East Bay
of San Francisco. Approximately 3 month assignment, day/early morning shift
available working in a hospital lab. Requirements: At least 6 months of paid
experience in a hospital histology laboratory
Check with a colleague working in a hospital and ask for fresh tissue (for IHC)
or (+) pathological cases.
René J.
--- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: RE: [Histonet] Control Slides
To: Rene J Buesa
I will be out of the office starting 12/20/2011 and will not return until
12/27/2011.
In my absence please ask for Mary . If this is urgent or you need to speak
to me directly you can contact me on my cell phone number 858-472-4266. If
it concerns a Mohs to be scheduled you can e-mail me or
Dear Dave, we use the next protocol with pretty good results.
For diazotation reaction (Solution A):
Dissolve 7mg Fast Garnet GBC base in 1ml of methyl or ethylenglygol. Add 1ml
HCl 0.1N. Add drop by drop 2.07mg NO2Na. Keep T below 15ºC.
Solution B:12.5mg/ml Naftol ASBI phosphate in
I use FileMaker Pro software to keep track of our IHC controls. I created a
control tissue file that includes the following: Control #, Case #, Tissue
site, Diagnosis, DOS, Time in formalin, Processing date, Number of blocks
submitted, and then a Comment field for the immunoreactivity for
Hi Does any one has idea about how to make in house gram Positive and negative
control in your lab. What kind of tissue is suitable and and i need
detail procedure for that.Thanks
--- On Wed, 12/21/11, histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu wrote:
Haloha!
Does any one has any idea about how to cut the fat tissue in cryostat (frozen
section). Temperature of the chambre/specimen and the thickness of the slice. I
use the cryo spray but does not help me much!
THX Miha and Mary C. 2 all
From: naveeda arshad
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