Time off is one thing but holidays are another. Holidays need to be rotated.
Seniority should apply to things like choice of hours but not holidays.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela
Hi all,
I was going to
prepare a solution 1% in distlled water of dodecaphosphomolybdic acid as
histological mordant
for Mallory staining. I noticed that it was not completely soluble in water, as
the Chemistry says,
but in the yellowish
solution there was a thin precipitate quite heavy.
So I
Sally,
This is a little different from what I understood yesterday. I thought that
it was stated you could only bill once per part, such as an S-100 on part
A,B,C would be fine, but not on A1,A3, and A5.
I did not read anything about only being allowed to order one antibody. I
thought panels
I'll seal the coverslip around all the edges and let it dry in the hood for up
to half an hour (because of the smell). I use a clear color that doesn't
fluoresce under the miscroscope and also allows the slide to be stored at low
temp (-20) without the nail polish seal cracking. Also be sure
Theresa
Although I agree with the messages on not trading quality for quantity
we ask our new grads to meet the following within the first 3 months.
Embedding 1 min/ block
Paring and cutting 2 min/block (average)
Some blocks are move difficult or require more levels and will take
longer and some
From what we understand from the new policy the cocktailed antibodies, such as
PIN4, are not to be charged separately.
Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104
To clarify, the Formical we use is a formic acid/EDTA solution.
Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com
-Original Message-
From:
I find it amazing sometimes when you don't do something for awhile how quickly
your brain throws the information away. That being said...I know back in the
day when I was learning histology we used to make our own acid alcohol solution
(now where I am had a butt load of Clearifier so I was
If you are talking about a regressive HE stain then perhaps it was 1% HCL acid
in 70% alcohol
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Thursday, January 05, 2012 11:00 AM
To:
You can use either acetic or hydrochloric, both will do. The strength is 1% BUT
it does not matter. As a matter of fact the weaker the solution the better.
Remember that the acid solution (that can be made with 7ethanolanol even
better) is used to differentiate progressivesive hematoxylin, like
I still use that. It is 70% with 5mL of hydrochloric acid if making 1L.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah
We use .5% acid alcohol ( hydrochloric) with Harris hemo. No problems.
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
I'm just full of questions today!! This one is IHC...I have been trying to
optimize a Caspase3 stain for several months now and it is still just chalked
full of background gradoo. I do all the blocking including Fc
receptors...still junk. The clone I have been using is, from abcam (ab2302).
You wrote:
We are currently starting up some IHC on frozen tissue sections. After
staining with different fluorescent antibodies, we end with applying DAPI
w/Prolong gold and then coverslipping. We would like to seal the coverslip
so that we can keep the slides longer. Any suggestions on
If you are looking to stain cleaved caspase 3 there is a better antibody from
cell signaling it works in multiple species, it's a bit pricey but I have found
that it works the best out of the few that I have tried. We have used it mouse
xenografts before without any issue.
Liz
Elizabeth A.
Does anyone have a pdf (or could fax me) the original paper for Elastichrome
stain:
Richardson, L. Combination Elastic Trichrome Stain, Laboratory Medicine, 6.1,
1975
We have a procedure with this reference, but no original paper to look at. We
do it very rarely and no one seems to know how
Tim
We do this stain all of the time, we never used the original reference we just
made up one on our own. I'll send our SOP in a different e-mail.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303)
I'll second that from our experience - Cell Signaling cleaved caspase-3
antibody works well on xenografts
Brett
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
-Original
Sarah,
I would use the 1% Acetic Acid in 70% alcohol, for a couple of reasons: your
docs are used to looking at the stain with the Clarifier, and acetic is the
ingredient in the Clarifier 2; also you probably will not have to change your
staining times by too much if you use acetic. You could
Tim,
I do not have the paper, but a few years ago I worked the kinks out of that
stain. Your acetic acid is too long. Try for the amount of time that you
usually do for a one step. A few dips would be a good start. Then rinse in
dH2O, not wash.
Also, I do not remember using the Weigert's and
Happy New Year to All,
I need some help from all of you out there in histoland.
How many of you wear respirators during your entire 8 hour work day for routine
histology? If you don't wear a respirator do you wear any type of mask or
shield at all for routine histology?
Also if any of you
Hello,
I have some paraffin sections that I've used for immunohistochemistry
(using an alkaline phosphatase-conjugated secondary antibody and
NBT/BCIP as a substrate), and coverslipped with an aqueous mounting
medium (Fluoromount G). I would now like to de-coverslip and re-stain
these
Hello histonet,
I have a protocol that calls for safran du gatinais and am experiencing some
serious sticker shock. Can I substitute saffron in my solution or am I asking
for trouble?
Many thanks,
Beth
Beth Villarreal
Scientist I
Novartis Institutes for BioMedical
Research, Inc.
300
No, not here. We don't wear anything at all. We have excellent air flow, and we
wear the badges that test for exposure to xylene and formalin annually which I
think is a CAP requirement. Our results have been better than acceptable every
year.
Lisa Brenner HTL (ASCP)
Histology Technical
Nothing at all???
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner
Sent: Thursday, January 05, 2012 3:22 PM
To: Amy Self; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Respirators
They must be in a liberal part of Michigan.
- Original Message -
From: Linda Blazek lbla...@digestivespecialists.com
To: Lisa Brenner li...@hollandhospital.org, Amy Self
as...@georgetownhospitalsystem.org, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Sent:
Hi Beth,
Saffron and safran du gatinais both refer to the dried stigma of the
saffron crocus. They are both saffron. I use and alcoholic extract of
Saffron to stain collagen in Movat stains. I've tried saffron from multiple
sources and price definitely does not always correlate with
Is anyone aware of a source for an antibody to CpiV - canine parainfluenza
virus. I have searched and come across some papers with IHC staining but I am
unable to access the complete paper.
Any help would be appreciated.
Thanks in advance.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Hi Beth, Saffron is a great spice. We use it in a old family recipe from
Cornwall for a bread roll( saffron nubbies). We also find the price very
expensive, but also varies a lot. If you know anyone in the middle East, or
India, they could get it a lot cheaper. We have raised it in our garden
I also do not wear any type of respirator. Not at any point of my day. I
annually wear a formalin badge to test for exposure rate, but thats it. I
gross under a fume hood and I use Slide Bright instead of xylene. It does
not have any fumes or noxious odor and is non toxic. My stain line is also
0.5% HCL acid ih 70 % ethanol
Rena fail
On Thu, Jan 5, 2012 at 11:25 AM, Burton, Lynn lynn.bur...@illinois.govwrote:
I still use that. It is 70% with 5mL of hydrochloric acid if making 1L.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
I'm in Texas and have been in histology labs since 1998 in 4 different places.
None of them did we ever wear a respirator for normal histology work. We did
have to wear masks when cutting frozens because of possible TB in lung tissue,
but that was it. Most labs should have some kind of
How old is the alcoholic Hx you use to prepare the Verhoeffs. I have found that
matured 10% ethanoic Hx is more resistant to differentiation than freshly
prepared Hx.
Might be of use
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior
Beth,
In the past, people replied to Histonet with the suggestion to buy saffron
aka safran du gatinais from a grocery store spice section. Depending on
where you are located e.g. a bigger city, try to find a store that sells
spices from India may have the freshest saffron. However, you
Sarah,
Your background is caused by cross-activity from your mouse primary
antibody on mouse tissue (xenografts). You can try Mouse on Mouse kit
(Biocare's has a great Mouse on Mouse HRP Polymer system).
I have used Cell Signaling's Rabbit anti-Cleaved Caspase 3 (Cat #
9661) on Xenografts and
When I work with xylene, it's always in the fume hood while wearing
gloves. The same goes for formaldehyde. I never use a face shield for
anything, I just do it in the fume hood if I need to use caution with a
certain chemical.
Emily
The whole point of this country is if you want to eat
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