Workhorse...has all the requirements we need.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Giracello,
Darlene
Sent: Thursday, February 16, 2012 12:47 PM
To: histonet@lists.utsouthwestern.edu
Subject:
We love ours!! Works great!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
susan.wal...@hcahealthcare.com
Sent: Friday, February 17, 2012 3:12 AM
To: darlene.girace...@okstate.edu;
Dear listers,
I am trying to get transversal sections of cow flexor digital tendons.
I need sections of the whole piece, about 1cm diameter. Before
paraffin embedding I have treated the samples with:
-KOH 4%
-4% phenol in 70% ethanol
In both cases the
I think the Ventana XT is the best instrument for adding slides as you go which
makes it perfect for a LEAN lab. This will reduce your TAT.The instrument is
so easy to use it is virtually impossible to mess up with it's bar coding and
visual control. Very user friendly.
Jan Mahoney,Omaha, NE
Side note: only the Ventana Ultra allows adding slides as you go, not
the XT.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice
Mahoney
Sent: Friday, February 17, 2012 7:52 AM
To: Christie Gowan;
For your requirements, Sakura VIP is the bast, at least for me.
René J.
--- On Thu, 2/16/12, Bales, Candy A candy.a.ba...@uth.tmc.edu wrote:
From: Bales, Candy A candy.a.ba...@uth.tmc.edu
Subject: [Histonet] tissue processors
To: histonet@lists.utsouthwestern.edu
First of all fixation has to be perfect, if with NBF, at least 72 hours. Then
increase twice your standard processing times, specially infiltration.
Substitute your normal paraffin was to one of 63-65ºC and chill be blocks
before sectioning.
René J.
--- On Fri, 2/17/12, Pablo Sanchez-Quinteiro
Great opportunity for a Histotechnician in a brand new laboratory!
Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice
located in Lafayette, Louisiana, is looking for a certified HT or HTL to
run their newly constructed laboratory. Candidate must be ASCP certified
and CLIA
Trying to put together some numbers, any and all responses to these questions
would be greatly appreciated. How many HT's are in union positions? If you
were applying for a position, how much of a factor would it be if it was a
union position?
Thanks in advance for your help!
We are not using negative controls. We only add positive controls at the bottom
of our patient slides. Sometimes there are 3 different types of control tissue
(a TMA), so that you can use the same control block for several antibodies. You
could add a negative control in the same block as the
Available through VWR, but may be cheaper elsewhere. They work much better
than I expected.
ZEROSTAT ANTISTATIC GUN VWR cat #100496-120 Vendor cat #60610
Each$122.64 list
Donna Harclerode, HTL, QIHC, SLS (ASCP)
Associate Scientist
Celgene
9393 Towne Center Road
San
Union position? Only if I was starving.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Bakken
Sent: Friday, February 17, 2012 10:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
We have 17 histotech over 1000 lab techs ( classified as Medical Technologists)
and NO union
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Eric, I wouldn't say my mind is closed on the issue and I appreciate your
thoughts and opinions. I agree wholeheartedly about the advantages of the XT
and to say that the Benchmark XT is a closed system is simply to state that it
is more closed regarding reagents than some systems (open) where
Happy Friday Histonetters,
We have a pathologist that will be ordering all the latest test such as MMR -
ALK Mutation and all of the other good ones that oncologist will be relying on
to treat their patients. He wants to order these test on cell block material
that he gets from FNA's. As is we
We are wondering what other labs are doing to control the humidity while IHC
stains are being performed. We currently place wet towels and a small weigh
boat with water in our Autostainer to help prevent our slides from drying
out but that seems to not be enough, they still tend to dry out and
A good auto stainer (like DAKO) with adequate amounts of dispensed reagents
during the correct periods of time should not experiment any drying out on the
slides. Adequate humidity is required to be controlled during manual IHC,
especially if done over a heated support. If because of any reason
We use the Dako Autostainer.
Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgo...@clinlab.com
-Original Message-
From: Rene J Buesa
Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying
out. South Dakota, in winter? 10 percent humidity and you get drying problems.
Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
-Original Message-
From:
Jason,
We've experienced similar problems a few summers ago when the environmental
conditions inside the Autostainers (dako) reach high temperatures and low
humidity. We keep thermometers inside the stainers to monitor these levels.
To remedy this problem, before every IHC run, I add a few
NY in winter has low humidity, I still have no problems with my Dako
stainer. Make sure the lid is closed. I had a tech who consistently left the
lid open, there was some drying on the first row of slides. Closed the lid
the problem stopped. Are you using Dako buffer? The surfactant in the buffer
I've had 3 Dakos and have had the drying phenomenon in all of them to some
extent. We put wet paper towels in the stain sink, as others have
mentioned. Plus, we found that most of the problem came from having an air
gap between the hood and the unit, which wasn't closed entirely by the
gasket.
We have the Lab Vision 360 from Thermo Fisher and we have really bad problems
with the slides drying out. We put wet paper towels and water boats in, but
after 20 or 30 minutes, the slides have very little liquid on them. I have
gone back a lot to manual staining except for stains that work
Pam,
I believe only the city morgue on Oahu have the histotech-type positions in
Hawaii that are union. There are about 48 histotechs or histotech-like
postions in 14 centers across 4 of 8 Hawaiian islands (the other 4 islands are
too small to support full medical facilities-and one is still
Hi; We had the same problem, but are in the dry state of Colorado. I have a
couple of suggestions. Make sure that the seal on your lid is adequate, as
overtime they can rot. Also, make sure that your racks are not warped. Warped
racks can cause some of the liquid to leak out of the sides.
Thanks for correcting me.Jan
Subject: RE: [Histonet] Ventana xt
Date: Fri, 17 Feb 2012 08:23:33 -0600
From: lseb...@uwhealth.org
To: mamaw...@hotmail.com; christiego...@msn.com; dphill...@vetmed.lsu.edu;
histonet@lists.utsouthwestern.edu
Side note: only the Ventana Ultra allows adding
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