But the gloves in that Ansell list are reusable, whereas I suppose that you
mean the disposable Kimberley-Clark gloves? The thickness would be very
different in that case. And I wouldn't be able to mount a slide with thick,
re-use gloves. :)
In any case, the doctor for my professional yearly
? You use regular superfrost plus slides for temperature verification of the
thermal pads? The slides to use are the superfrost plus slides from Ventana
that have a qc'd strip on them that will turn black if the temperatures of
pads are at acceptable levels. How do you prove that with a plain
We all have heard the reports that xylene causes cancer. It is a carcinogen.
However only in cases where the user is extremely sensitive to xylene should
you worry about a little bit getting on your skin every now and then. Don't
bathe in it. Do not make it a habit. Wearing gloves
Dear Histonet Members,
I have been trying to set up a histology lab in our pharmacology
department in Konya, Turkey.
So far, I could only buy some basic stuff for cresyl violet staining in
rat brain tissues.
If you would like to get rid of some of your lab equipments and
materials, or if you
Hello Everyone!
I am interested to find out how people are cleaning their Bond Bulk Reagent
Containers. I don't need a procedure. I know how to do that. My question is
how do you clean these containers when you use them day and night and they
don't have an opportunity to be cleaned and
What's badly needed is a registry of death certificates of
histotechnologists. The thing I'd want to examine would be the
prevalence of myeloid leukemia and related diseases - known to be
elevated in workers exposed to benzene. If such a correlation were
found, it would mandate eliminating xylene
Message 5: xylene
Xylene is toxic and you should always wear gloves and a face mask when dealing
with it. Dermal absorption is a lot slower than inhalation, but contact with
organic solvents such as xylene is bad in general. With the issue of latex
residue left on the slide, try using nitrile
Try after cleaning them rinse them with 100% alcohol and using a hair
dryer! You really should purchase a spare set anyway, because sometime
there are issues with the nozzles and the bond will not run without
them. Your production would stop immediately and you would still have
to purchase
Xylene is toxic and you should always wear gloves and a face mask when dealing
with it. Dermal absorption is a lot slower than inhalation, but contact with
organic solvents such as xylene is bad in general. With the issue of latex
residue left on the slide, try using nitrile gloves. We use
Hi,
Can anyone recommend Adenovirus controls for paraffin sections. I can not
find a vendor that sells these controls any longer. Any help would be
appreciated.
Pat Karlisch
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This message (including any attachments) contains information intended for
As I failed to mention in my earlier post - the problem with a
registry is that many (most, in my personal experience) American
histotechnologists are completely uncertified, and would not appear in
any registry. One would hazard a guess that these uncertified techs
get the most xylene exposure.
-Original Message-
From: Jackie O'Connor b427...@aol.com
To: Jonathan.Cremer jonathan.cre...@med.kuleuven.be
Sent: Thu, Feb 23, 2012 8:39 am
Subject: Re: [Histonet] Does xylene cause skin cancer?
I grew up in histology before MSDS's and OSHA. We practically bathed in
xylene, which
Hi,
I am wanting to send out blocks to different sites across the country. My
main concern is the block melting or being partially warped due to the heat.
What is the most cost effective way of sending out numerous blocks when
considering the above stated concern?
Thanks,
H
FedEx overnight with a warning to keep from extreme temps. That should keep
them off loading docks.
-Original Message-
From: E V hairlesstur...@gmail.com
To: histonet histonet@lists.utsouthwestern.edu
Sent: Thu, Feb 23, 2012 3:08 pm
Subject: [Histonet] Paraffin Block Shipping
Does anyone have a good monoclonal antibody to Serotonin that they would
recommend for diagnostic immunohistochemistry performed on formalin-fixed,
paraffin-embedded human tissue? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen
Has anyone used this antibody on dog? If so which company did you use? Would
you be willing to share your protocol?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
Hi Margaret,
We have used CK-Lu5 in the past with great results. It stains dog, cat,
pig, cow, horse, goat, deer, bird, frog, hedgehog, snake, guinea pig, rat,
and bats so far. Use Proteinase digestion for antigen retrieval.
We did notice that in recent times the staining intensity diminished
Hi every one, I would like to get people's opinions on whether mail-in
cytology slides both stained and un-stained should be left out on the
counter top or be put in a refrigerator until the next day for the tech to
process them? We have always had a policy of leaving them in their cases
As far as I know Xylene is not a conformed carcinogen. On the other hand the
structure is close to that of Benzene which is a confirmed human carcinogen.
It's an aromatic hydrocarbon, so why take chances. It makes sense to 1)
minimize use. 2) Use fume hoods when possible. 3) Wear
Fed-Ex is good, but include a cold-pack in with the blocks. A common freezer
pack from the local drug store will do it - and is reusable.
FL
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie
So apparently this model of slide writer is no longer manufactured, at
least from what I can tell. My Leica rep says they don't make it anymore,
but I really need to get my hands on one. Normally, I'm not a big fan of
this printer for larger volume labs, but the way we make slides here at our
I have been having some health issues lately (understatement) Does anyone know
the best formalin resistant disposable gloves? I gross (skin only) under a fume
hood but the gloves still come in contact with formalin naturally. I am also
(OK, mostly) interested in the health effects involving
I would still use nitril gloves fir that. Nitril may be a little more expensive
but for our department it's the only way to go if safety is practiced. Also I
would recommend that you look at the Msds of all reagents you work with. Your
department should gave a book. While we are on the subject
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