Hello, if you send images, I can say something. And I also can send cd31
positive images from different vascular tumors.
On Sun, Mar 11, 2012 at 3:49 PM, Gudrun Lang gu.l...@gmx.at wrote:
Dear histonet!
Has anyone seen granular, fine dot-like, cytoplasmic CD-31 staining in
tumorcells,
*Happy Histology Professionals Day from everyone at Personify!*
http://www.nsh.org/content/histotechnology-professionals-day
If you are considering making a career change or if you are looking to hire
someone in the Histology/ IHC world please consider Personify.
Personify typically
Not sure what the rates are, but remember this-negotiate at least a 1 year
contract for the tech. Some POL's are hiring techs, bringing someone else in
to train and then releasing the tech.
Toysha
Message: 11
Date: Fri, 9 Mar 2012 20:55:58 -0700
From: Carlos Hernandez
Does anyone have a protocol for Undecalcified bone for IHC?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Undecalcified? How are you going to section it?
If you can section it, just use any IHC protocol for regular sections.
Good luck!
René J.
--- On Mon, 3/12/12, Jeffery Howery jeffery.how...@jcl.com wrote:
From: Jeffery Howery jeffery.how...@jcl.com
Subject: [Histonet] Undecalcified bone IHC
Hi Jeff,
If is it possible a few more specifics of how the tissue has been received,
processed and evaluated would help. Undecalcified bone sectioning
procedures vary and also what specific markers are you looking to do is
important.
Vikki
On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa
René Jeffery,
I don't see sectioning as that big of a challenge. I often thin section
mineralized bone sections ~7 microns with my Leica microtome. Although I have
a great interest in developing an IHC technique for mineralized bone, I have
never had a spare moment to carry out any
I have also used Surgipath hematoxylin (Gill III) for years, and am
having no problems at this time. My last shipment was about 3 months
ago.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Jeff,
It is most certainly possible to do IHC on undecalcifed bone sections
embedded in PMMA although not the easiest task. Sectioning is done on a
microtome that is powerful enough to cut the plastic and using tungsten
carbide knives. The key is total removal of the plastic from MMA
Thank you Gayle. Vikki
On Mar 12, 2012 1:04 PM, gayle callis gayle.cal...@bresnan.net wrote:
Jeff,
It is most certainly possible to do IHC on undecalcifed bone sections
embedded in PMMA although not the easiest task. Sectioning is done on a
microtome that is powerful enough to cut the
Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hi,
Our lab is currently transitioning from the Brown Brenn gram stain due to no
longer wanting to store picric acid due to its potential hazards. Our
pathologists have requested a gram stain for paraffin embedded tissue that
looks similar but does not use picric acid or ether. Does anyone
We have been having issues with our Autostainer since bringing it out
of storage. All parts are in excellent shape, according to the
technician that performed the PM. It worked fine for a month of so,
but then began malfunctioning after only a couple of staining runs.
Does anyone know of any (free) online testing for color blindness= ?
Does anyone have an alternate method that they have used to satisfy CAP
requirements?
Laurie
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
There's a circuit board on the arm near the barcode scanner that controls the
movement and orientation of the arm. If that circuitboard malfunctions, then
you can see some of the erratic behavior that you are describing. That's one
possibility I can think of that might cause that kind of
Anyone have a Protocol for Decalcified bone for IHC?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
cool !!
On Mon, Mar 12, 2012 at 8:49 PM, Jeffery Howery jeffery.how...@jcl.comwrote:
Anyone have a Protocol for Decalcified bone for IHC?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Stacy,
You can buy a complete Brown and Brenn staining kit from Newcomer Supply
which is very good, and not change your method. The picric acid/acetone
mixture is included and you wouldn't have to store stock picric acid nor
ether in the lab but just this small amount of Picric acid/acetone
Having problems with IHC slides not looking the same between runs. Does it have
to do with how long and at what temp they are in the oven for?
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the new health
610 Oakmont Lane
Westmont, IL 60559
Office: +
Not really. Time in the oven and temp is usually not an issue as long as the
tissue is well fixed/processed/infiltrated.
René J.
--- On Mon, 3/12/12, Courtney Pierce courtney.pie...@quintiles.com wrote:
From: Courtney Pierce courtney.pie...@quintiles.com
Subject: [Histonet] IHC Slides
To:
Stacy,
There are several Gram stain variants that do not use Picric acid.
Tworts variant uses fast green and neutral red after the crystal violet-Iodine
steps.
Brown-Hopps method uses Basic Fuchsin, Gallego's differentiator and 1.5%
Tartrazine after the crystal-violet steps, to stain the
Hello histonet,
Does anyone using CD34? if so, can you share the staining protocol with us? and
what are you using for antigen retrieval?
Thank you so much.
Chakib Boussahmain
HTL(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Please unsubscribe.
thanks
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hi,
I was wondering if anyone can share their LIVER Biopsy Processing Schedule
with me?
Thanks,
Ade
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
24 matches
Mail list logo
