[Histonet] CD45.1 CD45.2 IF on parrafin sections.

Dear All, I want to do immunofluorescence on formalin fixed paraffin sections, Can you guys help me sending protocol how to do it ? Thank you very much for your help. BW Anil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] IHC Processor Validation MUM1

1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some

[Histonet] Ihc validation

I am having to validate antibodies and was wondering if you have to do a parallel study or just use known positive and negative tissue? ~Sabrina~ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Re: undecalcified bone IHC

I might also add that Neil Hand is co-speaking with myself and Philip Seifert this year at the annual National Society for Histotechnology - Symposium/Convention in Vancouver B.C. Our workshop is titled: Resin Applications Forum: Methods for Processing, Special Staining, Immunohistochemical

Re: [Histonet] CD34

Becton-Dickinson Monoclonal antibody at 1:100 for 30 minutes after HIER (citrate at pH6) with placenta as control. René J. --- On Mon, 3/12/12, Chakib Boussahmain chak_...@yahoo.com wrote: From: Chakib Boussahmain chak_...@yahoo.com Subject: [Histonet] CD34 To:

Re: [Histonet] IHC Processor Validation MUM1

CAP has some guidelines as to the number of specimens and antibodies to test. I think to remember that the lowest amount was 25 specimens, but I do not remember about the antibodies but it makes sense to use only those your use to work with. René J. --- On Tue, 3/13/12, Bliven, Laura

[Histonet] RE: Histonet Digest, Vol 100, Issue 15

We also have a first generation Autostainer and I have had the same happen with my machine. I believe it is definitely a mechanical issue. The engineer that came out changed the motor driver unit and we haven't had a problem since. He also told me that it can also be the belt drive on the

[Histonet] Color blind chart request

Google Color blind chart. Then select images from the google menu. You will get what you want. Select the image and print on a color printer. Viola! Done. Stephen G. Ruby -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: Color blind chart request

You probably have to have someone administer it and verify it to make it acceptable for CAP, though. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephen G. Ruby Sent: Tuesday, March 13, 2012 11:37 AM

[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.

I will be out of the office starting 03/13/2012 and will not return until 03/19/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology

[Histonet] Re: Gram stain

Stacy Giroux (where?) asks: Our lab is currently transitioning from the Brown Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or

Re: [Histonet] Re: undecalcified bone IHC

Excellent! Happy to read the venerable Neil Hand is coming back to the symposium, always a great speaker! -Damien On Tue, Mar 13, 2012 at 10:50 AM, Jack Ratliff ratliffj...@hotmail.comwrote: I might also add that Neil Hand is co-speaking with myself and Philip Seifert this year at the

[Histonet] Re: Color Blindness Testing

Laurie asks: Does anyone know of any (free) online testing for color blindness? Does anyone have an alternate method that they have used to satisfy CAP requirements? Here are some online Ishihara plates quickly found through Wikipedia:

[Histonet] Re: Brown and Brenn

Stacy, I use a procedure that was published in the Journal of Histotechnology, Vol 25, No2, June 2002. *Modifying the Modification: How to Redden Shy Gram Negative.* It has worked for me consistently for many years without the picric acid. It does use some acetone. I get the ragents from

[Histonet] Brown and Brenn

You can switch to Tartrazine. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary

RE: [Histonet] Re: Color Blindness Testing

I've been watching this thread with great interest I have been in Histology for 30+ years now and am myself colorblind (at least according to the Unites States Navy). I do see color even though I could not pass the old card test (failed it 3 times). My problem is with shades of color. I

RE: [Histonet] Re: Color Blindness Testing

What a great story :-) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, March 13, 2012 12:22 PM To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:

[Histonet] H.T. Job Opp in San Diego

Possible full-time position at a cancer diagnostic CLIA/CAP lab in San Diego: Must be H.T.(ASCP) Must have excellent cutting skills Must have IHC experience (we use the BondMax) LMD experience a plus or desire to learn (a must!) prefer local candidate   email resume to:   paw...@yahooo.com

[Histonet] End-point of decalcification vs. Ion exchange resins

Hi, I've read at a couple of places that you can't use the chemical test to determine the end-point of decalcification if you used an ion-exchange resin for the decalcification. I understand that calcium ions are captured by the resin, therefore they can't be precipitated by the ammonium

[Histonet] IHC Stainers

Hello Techs,   I would like to get some feedback on the Dako Autostainer Link 48, using the Flex reagents.   Thank you, Cindy  Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] End-point of decalcification vs. Ion exchange resins

If you do that all the advantages of using the ion-exchange resin will be lost. Simply determine the end point of the decalcification by other usual and more traditional methods, like specimen bending or a pressure/penetration test. You could also use X-ray with a Faxitron. René J. --- On Tue,

[Histonet] Thermo Richard Allen Screen Cassettes

Looking for help againHas anyone been experiencing problems with the ink printing coming off the Thermo Histoscreens after processing? This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in

[Histonet] CPT Code Question

Happy almost Hump Day Everyone, Does anyone use CPT code 88399 or 89240 for any type of unlisted or miscellaneous pathology test or procedures? If so what type of test/procedures are you using the code for? Also is there a CPT code that can be used for gathering up pathology material for

[Histonet] Greetings, friend!

Greetings, friend! http://metalgeek.zxq.net/response.php?urjluqjbahuma=79gjcewip=977ygoxab=38 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Re: Color Blindness Testing

Almost exact same situation with me Tom. Navy refused my application for Nuclear Engineering and made me a Medical Technologist instead. It struck me as funny the first time I had to identify an eosiniphil. Then I went to histolgy. Apparently the Navy didn't want me around things that could

Re: [Histonet] CPT Code Question

A long time ago I tried to use 88399 misc for prep work my techs were doing for various test we sent out. We never got reimbursed for anything on that code. Maybe others here had better luck than me with it. You seem to be thinking a long the line I was about all the work we do that we don't

Re: [Histonet] IHC Stainers

I love it. Great turn around time. Great crisp clean stains. And I think it's easy to use. Some will say ah it has the off line Retrieval. But I like the fact that the samples are submerged into a solution. Call me old fashioned? I've used pretty much all of the platforms. They all have good

[Histonet] ventana uv alkaline phosphatase red

for those of you that use the ultraview alk phos red on their machines, do you run the slides through alcohols? we are having a lot of trouble with the staining, sometimes it is good others no staining at all on our mart 1s or pin4 prostate bxs. I was told that we should not put them in

[Histonet] Did I mention...

Hi everyone - Did I mention that Damien Laudier from Laudier Histology was a guest last Sunday, March 11th on HistoTALK? Or that Vinnie Della Speranza from MUSC Health will be our guest on Sunday, March 25th? No? Well, Damien Laudier from Laudier Histology was a guest last Sunday, March 11th

RE: [Histonet] Re: Gram stain

I agree with Bob wholeheartedly as well as agree 100% and I am unanimous in that! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road