Hi Carol Freeman,
I work in a TMA lab in Hawaii. We own, and highly recommend, Pathology Devices
semi-automated TMArrayer and a Beecher TMA-1. However both are more expensive
than the routine pathology lab can easily afford. If you Google "TMA arrayer"
and you will get severa
It's pretty much up to you,. your boss, the pathologists what colors you
use. Red for rushes for example. The colors help identify a group of
specimens quickly, then they can be sorted by number. This is especially
useful if your SOP is to cut groups of specimens in a specific order.
Rena Fail
Bernadette,
There isnt a set in stone formal by law guideline for this. Its a user
preference. You can make up what ever colors you like for whatever criteria is
specific to your lab. So as you see , you got different answers. Dont fret
though, just figure out what your labs specific needs are
The National Society for Histotechnology is bringing the 5th Annual Summer
Symposium back to Las Vegas, NV! General sessions and workshops featuring
expert speakers will provide you with the tools, advice and guidance you seek
in your professional career. The Summer Symposium is one of the bes
Lyn,
If you are talking about small GI biopsies, we have a good protocol. We have a
VIP tissue processor that has 3- formalins, 1- 70% ETOH, 1- 95% ETOH, 3- 100%
ETOH's, 2-xylene's and 4- paraffins on it. Our GI biopsies are usually already
fixed in formalin before we load them so we skip al
I am not familiar with what IHC world has on this but we have used this
technique for a number of years. The mouse fragment we use is from Jackson cat
#115-007-003. We dilute it 1:10 in our protein blocking solution. It works
better with DAB than with Fluorescence. We have also found that to ge
So true, the person who found it the funniest was the Master Chief at
Personnel, once he told me the whole story of what happened and wiped the laugh
tears from his eyes. He warned me to keep a very, very, very low profile for
awhile, which I passed on to you, Jesse and the rest of our crew.
-
We were safe as long as their egos were intact.
-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Friday, March 23, 2012 9:34 AM
To: O'Donnell, Bill; Kara Lee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank
The one that almost
The one that almost got us in a lot of trouble was the one that we had everyone
convinced that I had been TDY'd to Quantico instead of going on leave. The best
part about that one, was while the Officers in charge were running around
trying to figure out who changed my orders from leave to TDY,
It seems to me that this is somewhat guided by the funds you have to
spend on a cassette labeler. Otherwise, if you label by hand, no
problem. Also, the ordering factor can become a bit more detailed if
you have a varied protocol. We keep it pretty simple; white being for
single levels, greens fo
Are you doing paraffin or frozen? We do quite a lot of frozen colon / ileum so
I can give you our protocols if you like.
Sonya
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Histonet@lists.utsouthwestern.edu
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About my previous post:
My employer is looking to buy an anatomic pathology lab with frozen section
service. Does anyone know of any labs for sale?
I am located in FL and southeastern labs for sale is what we are interested in.
Thanks in advance.
--
Thank you,
Marc Centofante
marccentofa...@gm
Hello all ~
If anyone could share their protocols for intestine processing, embedding,
cutting, etc I would greatly appreciate any input. I am having trouble getting
consistently good quality intestine sections and am always looking to improve.
Also, does anyone have an experience with a produ
No I understand fully what you are saying. Have a great weekend.
On Fri, Mar 23, 2012 at 10:10 AM, Lester Raff MD wrote:
> Thanks for the explanation! I didn't mean to jump on anyone, after all,
> it's Friday!
>
> Lester J. Raff, MD
> Medical Director
> UroPartners Laboratory
> 2225 Enterprise
Thanks for the explanation! I didn't mean to jump on anyone, after all,
it's Friday!
Lester J. Raff, MD
Medical Director
UroPartners Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel 708.486.0076
Fax 708.492.0203
-Original Message-
From: histonet-boun...@lists.utsouthwes
My Bad for not explaining fully - not all prostate biopsies do get PIN4,
additional sections are cut and saved for possible future requests on
prostate biopsies. This cannot be done automatically for the very reason
you describe. My intention was to point out that we separated these out so
that t
"Lavender - Prostate biopsies (short run also PIN4 built into protocol)"
No! No! NO! This is why we are running into trouble on
reimbursements!!! PIN4 should NOT be done routinely. If you mean
cutting a slide for potential PIN4, that's fine, but no pathologist
should automatically be doing a PIN
Hi Bernadette,
I know of several different color code set ups.
Pink - breast tissue (usually has separate processing cycle for ER/PR)
Lt. Green - biopsy tissue (has separate processing cycle - short run)
Tan - bone marrow (has special stains and IHC built into the protocol)
Lavender - Pr
Good Morning and Happy Friday fellow histonetters :)
I am looking into getting a Tissue Micro Array Device to make some nice
multi-tissue (normal) and multi-tumor blocks for controls, validations and lot
checks for IHC. Has anyone any advice for purchasing one, any favorites or any
we should
This doesn't really help solve your problem, but I have a question. Why
are you using two secondary antibodies?
Why not just use the conjugated one?
Emily
(starting Friday off right by eating candy for breakfast. I love Fridays!!)
The whole point of this country is if you want to eat garbage,
I am not aware of a standardized way to color code cassettes.
I have worked at several labs and it is individualized to each lab
needs. Currently we have red for stat or special stain, white for large
specimens, blue for biopsies, green for autopsy, yellow for bone, pink
for cell blocks, ora
There is no standard for color cassettes. That will be your choice. I used the
following:
Green for "rush" specimens. Yellow for skins. Pink for bone marrows. Blue for
autopsy specimens. Violet for gastric biopsies. White for routine specimens.
Prepare something like this and submit it to your pa
Good day histonetters.We are a new university hospital and setting up
histopatholology lab.I used to work with white biopsy cassettes only but not
technicolors.Got this boss who ask me protocols on colored cassettes etc...No
idea about this.Is there any standard pattern which i can just base a
I've been trying out the method from IHC world to use mouse primary plus
anti-mouse secondary abs on mouse tissue.
Protocol;
Fresh frozen sections (mouse spleen) cut 8um and air dried
Fixed acetone 10min
Wash PBS
Block with 2.5% normal goat serum in PBS/Tw, 30min
Block with unconjugated Fab frag
Hi all,
I've just purchased Texas-Red conjugated tomato lectin from Vector and have
been trying it out on some frozen mouse liver sections but cant see anything.
Protocol - fresh frozen sections 8um cut and air dried overnight. Fixed (10min)
acetone, washed PBS, tomato lectin applied (I've done
I am not sure how to post to histonet but if I email this address and then
it gets posted here is what I would like to post:
My employer is looking to buy an anatomic pathology lab with frozen section
service. Does anyone know of any labs for sale?
--
Thank you,
Marc Centofante
marccentofa...@g
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