A canary? Well, I know what you used that for...how did that work out for
you?
Actually, I know the answer because you're alive.
EH&S should really look into this option, screw the badges...
Emily
The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a
Yeah but they used to use canaries in mines to warn of toxic levels of
gases; having one in a histo lab might be a VERY good idea!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Sent: Friday, May 1
I've always had at least one; makes the day more tolerable.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz
Sohrab
Sent: Friday, May 11, 2012 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [
You are also likely to have spheres dissolve in toluene based mounting
media. Aqueous media may be the answer unless the neutral red runs out of
the tissue in the aqueous environment. Hard to get the best of both worlds
if dye runs in aqueous media and spheres dissolve in solvent based . Goo
Hate to say it, but yes plants are considered infectious. Thats why you cant
take them in ICU's either. I guess the mold or bacterias can grow on them. Most
places let this slide, but some dont. Good luck!
From: William
To: Behnaz Sohrab
Cc: ""
Sent: Frid
I keep a pothos and spider plant in my lab. EH&S has never complained,
though I can't say one way or another if it's technically allowed.
While my plants are mostly just decorative (I don't think I have enough of
them to make much of a difference), it doesn't hurt that they may be
filtering our ai
When it is time to exchange reagents, the paraffin is the first thing we do
because of the melting time required.
We fill up the bin and place it in the oven until there is room to add more wax
pellets - we do not stuff it in, but leave it rather loose. We do this about
six times during the day
Thanks for your kind responses!
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Freitag, 11. Mai 2012 19:59
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] para
This is probably a best case scenario, but for labs like the one I am currently
in I melt it in the processor. My lab doesn't have the funds to buy me a
melting pot (I have had them in all my other labs, just don't know what they
are technically called).
To answer your question it usually takes
I agree Rene!
I also believe Sakura recommends not putting paraffin flakes directly in
the containers.
On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa wrote:
> Regardless of the time it takes or of how many people do it, melting the
> paraffin directly in the VIP should not be done because it ca
Regardless of the time it takes or of how many people do it, melting the
paraffin directly in the VIP should not be done because it causes the heating
elements to work extra reducing their useful life. They are quite expensive to
replace!.
Melt the paraffin outside the VIP and use the VIP only
After staining dry the sections in an oven at 60ºC for 10 minutes. When
completely dried, coverslip as usual. Beware of the mounting medium solvent
because it may contain xylene as well. Use one mounting medium without xylene.
René J.
--- On Fri, 5/11/12, Andrew Coleman wrote:
From: Andrew Co
I think that it is called Crystal Mount - but apply to section, allow to
harden dip slide in clearing media and coverslip. I know that there must
be others out there as well.
Good Luck
On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman wrote:
> Hi all,
>
> We are performing a neutral red counte
It's probably more toxic for the plants, but I like having them and no one
has told me I had to remove them. Ivy's are the most sturdy and the green
color just perks up things.
On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab wrote:
>
> I was told by infectious control person that plants are not a
HI!
A question for those, who melt the paraffin directly in the VIP. How long
does it take to melt the pellets in the VIP-oven?
Thanks
Gudrun
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Andrew
You could use Clearium from Leica. Clearium can either be coverslipped from
xylene or isopropyl alcohol. Drying time from isopropyl is longer then
xylene.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
-Origina
I have had plants in a number of labs. Could be against the rules, but I never
saw it. I even had a canary in one lab - pretty sure that is against the rules.
Will Chappell
Sent from my iPhone
On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" wrote:
>
> I was told by infectious control person th
I was told by infectious control person that plants are not allowed in the
lab?? IS this true? any experience with this?
Thank you, Behnaz
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Andrew
There are aqueous permanent mounting medias such as Advantage Permanent
Mounting Media from Accurate Chemical NB300A (516) 333-2221 its been years
since I used it but it does work on some applications.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 1
Hi all,
We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres are inert to
alcohol, but are washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and me
You can get the old-style sakura baskets from this company. These are the ones
in which the plastic cassettes lay on their sides. They are about half the
price.
*
http://store.techonebiomedical.com/store/
We sell the old style Sakura baskets. We have the 50 and 100 cassette m
Yes, most testing is performed in the Microbiology Laboratory; however, I have
an IHC test for formalin-fixed, paraffin-embedded tissue.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartf
What stain is anyone using for cryptosporidia in histology?? I thought this
was more of a microbiology test. Could use some help please!!
Thanks,
Dorothy Webb
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