What I used to do was when I got a thick specimen we would prepare 20-30 slides
and run one of those each day for a positive control. We also ran a blank slide
as a negative control to insure we didn't have contamination in our reagents.
Doing these two things were part of our lab to meet CAP
DAKO IHC guide book.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
We make a buckle smear and stain it. Quick and easy. The QC is for the pap
stain quality.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pathr...@comcast.net
Sent: Monday, June 11, 2012 8:49 AM
To:
Good Morning!
Does anyone have any extra giemsa stain control tissue that they would be
willing to share?
Thanks!
Gale
Gale Limron CT,HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562
This e-mail is intended only for the person or entity to
Why would you need a Giemsa control?
ANY tissue, but especially tonsil or bone marrow section, will stain with
Giemsa if done properly.
It is not a tissue you will need, but a correct procedure that will stain
properly a test tissue.
René J..
From: Gale Limron
Hi All,
I have my own consulting company now. Histology ProConculting, LLC. I set
up histology, derm or Mohs labs. I have 40 years+ experience with 20 years
of it in derm labs. I have written procedure manuals and kept them CLIA
compliant. I can train Mohs techs. I have completed my last project
Anyone have tips for quenching endogenous peroxidase in eosinophils? Our
standard px block is not doing the job (Biocare Peroxidazed-1).
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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I want to know what happened to all the equipment there, especially the large
Sartorius Microtome that was in the bone lab. If anyone has that information
please email me.
Shirley
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Histonetters!
I hope everybody is having a great start to the week. I have some new
positions to tell you about. All of the clients that I represent offer full
time permanent positions with excellent pay and benefits They are ready to
interview and hire right away! Here are my newest and
Are there any labs in the Atlanta area that offer Intern positions in paraffin
histology? After graduating from a formal histology program I worked in
electron microscopy then moved on to calcified bone and implants, therefore,
all of my working experience has been with plastic resins. I would
For trichrome: appendix is the most universal control I know of. Then maybe a
kidney.
GMS: store bought controls are nice, but if you live close to the local morgue
try them, every so often they get a good case that they can spare some.
Hemosiderin: Fetal Liver is good, or as you say
Does anyone know of a company that sells Uro-2 control slides?
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Cell Marque sells thempart number 345S.
Thank you!
Brandon Pineau
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pathr...@comcast.net
Sent: Monday, June 11, 2012 12:25 PM
To:
Rene - Those were STUFFED armadillos that had escaped from the Smithsonian!
The real armadillo work (with leprosy) is still going on in Hawaii ('cause I
got involved in that for a very short period of time in the Olde Days). If you
want an armadillo - ask someone that lives in Texas and I'm
Thanks! Nice to know.
René J.
From: nmhi...@comcast.net nmhi...@comcast.net
To: Rene J Buesa rjbu...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu; Shirley A. Powell
powell...@mercer.edu; Jay Lundgren jaylundg...@gmail.com; Bob Richmond
rsrichm...@gmail.com
Is that what you get to do in retirement, I am working on my second one,
hopefully I can start ignoring now.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-Original Message-
From:
Of course I know AFIP closed, I was there the day they shut the doors.
That doesn't answer the question of what happened to the armadillos. I'll
bet someone put them in a box with a blanket and gave them away in front of
Walmart.
I'll bet you a nickel she means H. pylori controls. Good luck with that
one Gale. I worked in a lab that is sitting on 100 lifetime's worth, but
it's a secret... (hint: inadequate sanitation, low social class, and
crowded living conditions are related to H. pylori infection.)
Dear Kim
I attached the peroxidase block method via personal email which works for
either frozen or FFPE sections. You must buy the correct, exact glucose
from ICN as Sigma discontinued this. I included the original publication.
You can also find this blocking method on the Vector website.
Hey All,
Our version of the Histo Bath is on the market and ready to sell. No,
I'm not trying to get a plug in for it. The manufacturer was asking me
if there were any regulating bodies other than UL and CE etc. that they
should register with. ex. CAP and JCAHO. Any thoughts on the matter
Hello Histonetters,We are looking for HPV positive control tissue for ISH
testing for both high risk and low risk probes. Does anyone have a half dozen
or so slides of each that they could spare? A block would be better but we'll
take whatever you could spare. If you have something you
Hello Histonetters,Does anyone use inventory management software to keep track
of their chemical inventories as well as other supplies? We are working on
setting up Kanban cards but we are wondering what other labs are doing to help
with inventory and ordering. Thanks for your
Hi,Our current protocol for preparing cytospins from body fluids and cytospins
is very limited. We basically add fixative and spin it down. This leaves us
with pretty bloody slides and cell blocks at times. In my past experience, we
did a lot more to the specimens and I think the blocks
Okay, now I have to know more. I realize this is not strictly
histologically-oriented, but since armadillos are used for research that
would likely involve histology, I'd like to know more about the armadillos used
at AFIP. And I assume that by putting them in a box with a blanket... that
Could this be something along the line of The Armadillos of Walmart? Sorry -
can't resist.
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Armadillos (Dasypus novemcinctus) are more or less unique in being
easily infected with human leprosy; in fact, about 2% of Louisiana
'dillos are naturally infected. Obviously these animals would be quite
an infection hazard. What I want to know is - is there any way to
obtain armadillo tissue
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