Re: [Histonet] cytology control slides?

2012-06-11 Thread Kim Donadio
What I used to do was when I got a thick specimen we would prepare 20-30 slides and run one of those each day for a positive control. We also ran a blank slide as a negative control to insure we didn't have contamination in our reagents. Doing these two things were part of our lab to meet CAP

RE: [Histonet] QIHC exam

2012-06-11 Thread Bea DeBrosse-Serra
DAKO IHC guide book. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] cytology control slides?

2012-06-11 Thread Boyd, Debbie M
We make a buckle smear and stain it. Quick and easy. The QC is for the pap stain quality. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Monday, June 11, 2012 8:49 AM To:

[Histonet] giemsa stain control

2012-06-11 Thread Gale Limron
Good Morning! Does anyone have any extra giemsa stain control tissue that they would be willing to share? Thanks! Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to

Re: [Histonet] giemsa stain control

2012-06-11 Thread Rene J Buesa
Why would you need a Giemsa control? ANY tissue, but especially tonsil or bone marrow section, will stain with Giemsa if done properly. It is not a tissue you will need, but a correct procedure that will stain properly a test tissue. René J.. From: Gale Limron

[Histonet] consulting

2012-06-11 Thread mequita praet
Hi All, I have my own consulting company now. Histology ProConculting, LLC. I set up histology, derm or Mohs labs. I have 40 years+ experience with 20 years of it in derm labs. I have written procedure manuals and kept them CLIA compliant. I can train Mohs techs. I have completed my last project

[Histonet] endogenous peroxidase in eosinophils

2012-06-11 Thread Kim Merriam
Anyone have tips for quenching endogenous peroxidase in eosinophils?  Our standard px block is not doing the job (Biocare Peroxidazed-1). Thanks, Kim   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list

RE: [Histonet] Re: best controls

2012-06-11 Thread Shirley A. Powell
I want to know what happened to all the equipment there, especially the large Sartorius Microtome that was in the bone lab. If anyone has that information please email me. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RELIA Hot Histology Job Alert Exciting New Opportunities.

2012-06-11 Thread Pam Barker
Hi Histonetters! I hope everybody is having a great start to the week. I have some new positions to tell you about. All of the clients that I represent offer full time permanent positions with excellent pay and benefits They are ready to interview and hire right away! Here are my newest and

[Histonet] Histology Intern Positions in Atlanta

2012-06-11 Thread C B
Are there any labs in the Atlanta area that offer Intern positions in paraffin histology?  After graduating from a formal histology program I worked in electron microscopy then moved on to calcified bone and implants, therefore, all of my working experience has been with plastic resins. I would

[Histonet] RE: best controls (Bob Richmond)

2012-06-11 Thread Mayer,Toysha N
For trichrome: appendix is the most universal control I know of. Then maybe a kidney. GMS: store bought controls are nice, but if you live close to the local morgue try them, every so often they get a good case that they can spare some. Hemosiderin: Fetal Liver is good, or as you say

[Histonet] Uro-2 controls

2012-06-11 Thread Pathrm35
Does anyone know of a company that sells Uro-2 control slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Uro-2 controls

2012-06-11 Thread Brandon Pineau
Cell Marque sells thempart number 345S. Thank you! Brandon Pineau -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Monday, June 11, 2012 12:25 PM To:

Re: [Histonet] Armadillos living at AFIP

2012-06-11 Thread nmhisto
Rene - Those were STUFFED armadillos that had escaped from the Smithsonian!  The real armadillo work (with leprosy) is still going on in Hawaii ('cause I got involved in that for a very short period of time in the Olde Days).  If you want an armadillo - ask someone that lives in Texas and I'm

Re: [Histonet] Armadillos living at AFIP

2012-06-11 Thread Rene J Buesa
Thanks! Nice to know. René J. From: nmhi...@comcast.net nmhi...@comcast.net To: Rene J Buesa rjbu...@yahoo.com Cc: histonet@lists.utsouthwestern.edu; Shirley A. Powell powell...@mercer.edu; Jay Lundgren jaylundg...@gmail.com; Bob Richmond rsrichm...@gmail.com

RE: [Histonet] Armadillos living at AFIP

2012-06-11 Thread Patsy Ruegg
Is that what you get to do in retirement, I am working on my second one, hopefully I can start ignoring now. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From:

Re: [Histonet] Armadillos living at AFIP

2012-06-11 Thread Jay Lundgren
Of course I know AFIP closed, I was there the day they shut the doors. That doesn't answer the question of what happened to the armadillos. I'll bet someone put them in a box with a blanket and gave them away in front of Walmart.

Re: [Histonet] giemsa stain control

2012-06-11 Thread Jay Lundgren
I'll bet you a nickel she means H. pylori controls. Good luck with that one Gale. I worked in a lab that is sitting on 100 lifetime's worth, but it's a secret... (hint: inadequate sanitation, low social class, and crowded living conditions are related to H. pylori infection.)

[Histonet] Re:Glucose Oxidase peroxidase block that works for eosinophils

2012-06-11 Thread gayle callis
Dear Kim I attached the peroxidase block method via personal email which works for either frozen or FFPE sections. You must buy the correct, exact glucose from ICN as Sigma discontinued this. I included the original publication. You can also find this blocking method on the Vector website.

[Histonet] regulating bodies

2012-06-11 Thread Matt Mincer
Hey All, Our version of the Histo Bath is on the market and ready to sell. No, I'm not trying to get a plug in for it. The manufacturer was asking me if there were any regulating bodies other than UL and CE etc. that they should register with. ex. CAP and JCAHO. Any thoughts on the matter

[Histonet] HPV Control Tissue

2012-06-11 Thread Kelly Colpitts
Hello Histonetters,We are looking for HPV positive control tissue for ISH testing for both high risk and low risk probes. Does anyone have a half dozen or so slides of each that they could spare? A block would be better but we'll take whatever you could spare. If you have something you

[Histonet] Inventory Management System

2012-06-11 Thread Kelly Colpitts
Hello Histonetters,Does anyone use inventory management software to keep track of their chemical inventories as well as other supplies? We are working on setting up Kanban cards but we are wondering what other labs are doing to help with inventory and ordering. Thanks for your

[Histonet] Body Fluid Specimens

2012-06-11 Thread Kelly Colpitts
Hi,Our current protocol for preparing cytospins from body fluids and cytospins is very limited. We basically add fixative and spin it down. This leaves us with pretty bloody slides and cell blocks at times. In my past experience, we did a lot more to the specimens and I think the blocks

Re: [Histonet] Armadillos living at AFIP

2012-06-11 Thread nmhisto
Okay, now I have to know more.  I realize this is not strictly histologically-oriented, but since armadillos are used for research that would likely involve histology, I'd like to know more about the armadillos used at AFIP.  And I assume that by putting them in a box with a blanket... that

[Histonet] Armadillos

2012-06-11 Thread nmhisto
Could this be something along the line of The Armadillos of Walmart?  Sorry - can't resist. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Armadillos living at AFIP

2012-06-11 Thread Bob Richmond
Armadillos (Dasypus novemcinctus) are more or less unique in being easily infected with human leprosy; in fact, about 2% of Louisiana 'dillos are naturally infected. Obviously these animals would be quite an infection hazard. What I want to know is - is there any way to obtain armadillo tissue