RE: [Histonet] human vimentin in mouse tissue

2012-07-12 Thread Jonathan Cremer
Hi Kim, I use Sigma V4630 in mouse tissue, but it is sold as an anti-human antibody (but reactive to many species). I use it at a dilution of 1:2500 with a PerkinElmer Cy3 TSA kit, with a Jackson HRP-conjugated secondary. Oh, before I forget, standard AR with citrate, pH 6. Hope this helps.

[Histonet] Epic Beaker module

2012-07-12 Thread Paula Sicurello
Good Morning One and All, Does anyone out there have any experience with the Beaker module of the LIS system EPIC? We have tests that require tables and charts to be inserted into the final report, pathologists need to have a case queue and other items. If you are using Epic/Beaker- please let

[Histonet] Ventana symphony

2012-07-12 Thread Work KCSCC
Can anyone give me information on their experience with the Ventana Symphony stainer? Nacaela Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Epic Beaker module

2012-07-12 Thread Martha Ward-Pathology
We would interested in any replies as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From:

[Histonet] Re: Methanol in H2O2 explanation

2012-07-12 Thread C.M. van der Loos
Hi, There is a paper from 1972 (!) by Streefkerk entitled: Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxidase following methanol. JHC 30:504. So perhaps the origin of methanol is not that mysterious. I cannot imagine that methanol is still considered for

[Histonet] HT/HTL Full Time Opportunities in New York state!

2012-07-12 Thread Brannon Owens
Allied Search Partners is working with different laboratories in the state of New York, looking for qualified and licensed Histotechnologists or Histotechnicians. To Apply Please email or fax resume to bran...@alliedsearchpartners.com mailto:bran...@alliedsearchpartners.com or fax to 888 388

[Histonet] ER, Clone SP1

2012-07-12 Thread Leigh York
Dako is going to discontinue the ER , SP1 clone. We really want to use the SP1 clone, but do not want to use an ASR. We can get it from Ventana only we are told. Can anyone elaborate on how they are handling this in their lab or if they have switched to an ASR? Thanks, Leigh York

[Histonet] Re: Methanol in H2O2 explanation

2012-07-12 Thread Hobbs, Carl
H202 mixed with Buffer here.(instead of aq.) That's OK too, right? Right. An addition to the misconceptions that Tim interestingly elucidates: some use H2O2 in buffer because they think that , as the enzyme is performing a physiologic catalytic reaction, it should be at a physiological pH, in

[Histonet] Beaker module in EPIC

2012-07-12 Thread Paula Sicurello
Hello all Listers, I've already asked the Histonet but I'm wondering about a different thing this time. Does anyone have experience with Beaker in conjunction with Anatomic Pathology, Surgical Pathology or the multiple other clinical labs that are in hospitals today? I work in Anatomic/Surgical

[Histonet] p63 and Biocare Medical

2012-07-12 Thread Jmyers1
Fellow Histonetters: Over the past few days there has been some discussion on this forum regarding Biocare Medical's ability to continue offering the p63 antibody, some of which has been correct and some of it incorrect. Please be assured that Biocare still has the right to sell products that

RE: [Histonet] ER, Clone SP1

2012-07-12 Thread Patsy Ruegg
From what I understand the SP1 Er clone has also been discontinued in USA by Leica/Novacastra, I am told it is still being sold by Leica in Europe? Must be another licensing issue because some are s greedy. I knew this was going to happen and stock piled some of the ER SP1, but it won't last

Re: [Histonet] ER, Clone SP1

2012-07-12 Thread Kim Donadio
I'm curious as to why all if a sudden some of these can be sold else where and not in the USA. Is it a FDA issue? Sent from my iPhone On Jul 12, 2012, at 5:45 PM, Patsy Ruegg pru...@ihctech.net wrote: From what I understand the SP1 Er clone has also been discontinued in USA by

RE: [Histonet] ER, Clone SP1

2012-07-12 Thread Morken, Timothy
It is most likely licensing issues between companies. A company may carry it under license from another company but the terms change and the licensee either loses the license (the other company won't license it), can't afford it or other issues. For example, when I worked in the antibody

RE: [Histonet] ER, Clone SP1

2012-07-12 Thread Patsy Ruegg
Yea you are right Tim, I just wonder what ever happened to trying to get the best ab for the patient, SP1 is in my opinion the best ER ab but now the patient suffers because we can't use what is best for them, there is something wrong with that picture. Patsy Ruegg, HT(ASCP)QIHC Ruegg Consulting