RE: [Histonet] Beaker module in EPIC

Again, we would be interested in any replies as well. Our institution is going live with Epic this fall but the labs will not be using Beaker until later on. We are still trying to figure out how all this will work and welcome all information. Paula, how soon will Duke be up and using

RE: [Histonet] ER, Clone SP1

I love the comparison :-) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jonathan Cremer Sent: Friday, July 13, 2012 1:45 AM To: Morken, Timothy; Kim Donadio; Patsy Ruegg Cc:

[Histonet] Different Avenues To Become Certified

 Good morning Histo-Land, A question was asked to me by my Medical Director the other day, so I ask for everyones expert answers to this question.  Is there any other entities other than ASCP to be certified by for HT/HTL ?   I thank everyone in advance. Have a great weekend. BEST REGARDS,  

[Histonet] TRAP staining

Currently we are trying to use Sigma Kit 386A for Trap staining of FFPE rabbit spine.and getting no staining , only tons of gold background. Anyone have experience with this procedure? Thanks Mary Sturm Notice: This UI Health Care e-mail (including

[Histonet] RE: TRAP staining

What was your tissue decaled in? Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Re: Methanol in H2O2 explanation

This is the real explanation. Mind that the title refers to erythrocyte pseudoperoxidase inhibition and in the title of the article is the procedural sequence: 1- fix the blood smear with methanol, which is the standard procedure, and 2- apply H2O2 to quench the peroxidase activity. René J.

Re: [Histonet] Re: Methanol in H2O2 explanation

I have been fascinated by this long discussion about H2O2 and its use in IHC procedures. We have to start from the fact that ALL cells contain peroxidase because ALL cells respire and ALL cells need to use oxygen they receive from blood (or hemolymph in crustaceans and other arthropods). The

RE: [Histonet] Re: Methanol in H2O2 explanation

I think that the original question was why is it recommended in some procedures that H202 be diluted in methanol. And others say distilled water or buffer. What makes one better than the other? Some people I have worked with insist on getting 30% h202 and diluting it in methanol to a 3%

RE: [Histonet] Re: Methanol in H2O2 explanation

We use hydrogen peroxide in a sodium azide solution,(on acetone fixed GMA processed tissue) works fine. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: 13 July 2012 15:18 To:

[Histonet] H2O2 Recep

So what I'm gathering from all this. Thanks by the way for the discussion. Methanol is unnecessary for the H2O2, reaction, but it can make it slightly faster since both methods will be working in concert. If you have tissue that has more peroxidase than usual, then extra time is required.

[Histonet] RE: Methanol in H2O2 explanation

Patrick Lewis wrote: So what I'm gathering from all this. Thanks by the way for the discussion. Methanol is unnecessary for the H2O2, reaction, but it can make it slightly faster since both methods will be working in concert. If you have tissue that has more peroxidase than usual, then

RE: [Histonet] Beaker module in EPIC

I'm curious. Are ANY labs using Epic for their APLIS? The last I heard was a sales presentation a couple of years ago where the sales person declined to show the APLIS because it wasn't ready. I do know of hospitals using Epic for order entry, though. Michael Mihalik PathView Systems | cell:

Re: [Histonet] H2O2 Recep

The bubbling of H2O2 frozen tissue is caused by the chemical destruction of the tissues. Do you remember when you apply 3% H2O2 to a skin wound? Don't you remember the bubbling it produces? That is the oxygen destruction of the white blood cells that constitute the pus in any wound, as well

RE: [Histonet] Beaker module in EPIC

So far we have not had any responses so I assume the answer is no.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: Michael

[Histonet] Re: Histonet Digest, Vol 104, Issue 15

All, ER, clone SP1 is availuable in www.epitomics.com (cat. #4200-1) for $205. Hope this help! Madeleine Huey Supervisor - Pathology (IPOX Histology) madelein...@elcaminohospital.org On Fri, Jul 13, 2012 at 7:08 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list

[Histonet] slide/block combo mailer

What company is making/distributing the new One Slide/One Block combo mailer? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] ER, Clone SP1

I need to correct my statement about ER Sp1 being sold in Europe by Leica after taking it off the market in the USA, I was confusing this ab with the HER2 CB11 that Leica was still selling in Europe and not the USA, they had to take it off the market in US to get FDA approval which they apparently

[Histonet] Histology Project

Hi everyone, I need someone to do these things for our future Derm lab. Please email me the cost and how long it would take. Chemical Hygiene Manual, the Exposure Control Manual, the Procedure Manual and the Q.C. Manual to CLIA standards. Regards, Jade Ryan, MHA, BSN Sent from my iPhone