RE: [Histonet] Sections washing off slides

2012-07-27 Thread Mehlika Faire
Have you tried baking or placing your slide on a slide warmer for a few hrs or overnight prior to staining? That seems to help make the sections stick.I would do this step after transferring the section to a charged slide. -Mehlika > From: kst...@mcw.edu > To: histonet@lists.utsouthwestern.edu

[Histonet] Opinions regarding Avantik RVG1

2012-07-27 Thread Carl
Hello Everyone, What has been your experiences with the company Avantik? Have you used their processor the RVG1 and what has been your experiences? Any input would be great. Regards, Carl Sent from my iPad ___ Histonet mailing list Histonet@lists.u

RE: [Histonet] Inking Skin

2012-07-27 Thread CHRISTIE GOWAN
Just to clarify: We do ink our excisions as described below in Cindi's response howerver, our MOHs specimens come to us mounted on a pieces of sturdy paper and are processed in place. We only describe them at gross and then directly place the paper and skin into a cassette for processing. The s

[Histonet] Sections washing off slides

2012-07-27 Thread Stoll, Kathryn
I have some old H&E slides that I would like to try IHC staining on. I am having trouble keeping the tissue on the slide. I put a slide in xylene to remove the cover slip and eventually the section floated off the slide when I tried to rehydrate. The second time I tried Mount-Quick from Newcomer

[Histonet] Inking Skin

2012-07-27 Thread CHRISTIE GOWAN
Dear Colleagues, My derm pathologist has requested that we ink the epithelium on MOHs derm cases (new pathologist). We process many derm specimens a day but it seems we are having trouble getting full epithelium on our cases that come over from the MOHs clinic. I am not sure that inking the ski