Dear Histonet members,
Good afternoon! I was wondering if anyone can kindly recommend a
free-standing electronic dictation system to be used with an Anatomic
Pathology information systems? Any suggestions or advice would be
greatly appreciated.
Thank you,
Mari
Mari Yang, MHA, CT(ASC
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: "Coskran, Timothy M"
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Wed, 8 Aug 2012 17:10:20
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ki67 to stain mouse tissue
We've had good success with
We've had good success with clone SP6, a rabbit monoclonal from Vector. This
antibody works in many species and has been very reliable.
Tim Coskran
Pfizer
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We just add about 10-15 ml of eosin in the last alcohol on the processor
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[
Eva, et al,
Yes, the LabVision/ThermoSci rabbit polyclonal does stain mouse tissue.
I've done validation trials on various species with the antibody and mouse
was one of many that did react.
Jan Shivers
UMN Vet Diag Lab
On Wed, Aug 8, 2012 at 10:51 AM, Eva Permaul wrote:
> I just found a nice
We have swithched from the VIP Sakura, older models, to the Thermo Excelsior.
We are currently buying our 3rd and 4th units for the Histology Lab. They have
features we really liked and cut my our exposure to formalin and xylene by
almost 90% due to the way the system changes solutions. Due
FYI: That is what the data sheet says because they didn't test it but
many of us have and it works beautifully!
It should stain mostly in the bottom of the crypts where the new cells
emerge from but you will have occasional cells towards the top of the
crypt. It should look much like it does i
I just found a nice publication with the Rm-9106 on mouse intestine. Have
provided it to my manager. Thank you everyone.
Eva
On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul wrote:
> Just to clarify. Are you using it on mouse tissues? I am only asking
> because the Thermo Scientific/Labvision/Neomar
We make up a "marking Eosin" that we use on small bx before we place them in
cassettes.
We do not use actual teabags, but the mesh bags from Thermo Fisher that are not
hard to pull apart and we never have anything sticking I the corners. I do
know what you are referring to as some of the mesh
Just to clarify. Are you using it on mouse tissues? I am only asking
because the Thermo Scientific/Labvision/Neomarkers data sheet says it has
only been verified on Human tissues.
Secondly had anyone used it on mouse gut. Does it stain in the bottom of
the crypts or at the top? I am asking because
In addition to Rene's comment,to cut coagulated tissue (skin that have
new wound crust) and calcified tissue is difficult.
On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar wrote:
> Hi All
> I am trying to section adult mouse intestine and skin using paraffin
> embedding. However, when i section,
What you describe is a typical example of poor paraffin infiltration = the
paraffin has not infiltrated the tissue and when you prepare the final block it
will consist of 2 different components; the tissue and the paraffin. That is
why you end with a "good paraffin section" without the tissue.
P
Alice
I would recommend using sodium formate/formic acid mixture for demineralization
as this is more gentle than most agents.
I would not use hydrochloric acid unless you are shipwrecked on a desert island
and that is the only chemical available to you.
I am assuming that EDTA demineralization i
I always used few drops of alcoholic eosin in the 70%EthOL, just enough to make
the solution a "pale pink". That amount is enough to give a faint "pink hue" to
the tissue to ease its localization.
This does not interfere with any stain done after wards.
René J.
Sakura
René J.
From: "Heckford, Karen - SMMC-SF"
To: "histonet@lists.utsouthwestern.edu"
Sent: Wednesday, August 8, 2012 8:11 AM
Subject: [Histonet] Tissue Processor
I am going to need to purchase a new tissue processor mine keeps breaking
down. What tissue
We use microwave processing and we add hematoxylin to the absolute and eosin to
the isopropylthis also helps in keeping techs from accidently using
isopropyl as absolute or vice-versa... when grosser cant find tissue in the
container we put a drop of eosin and swirl and filter to try and fi
I am going to need to purchase a new tissue processor mine keeps breaking down.
What tissue processor would you buy and why? I would greatly appreciate the
help.
Cheers,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-
ditto on this. I also have very tiny specimens and this works wonderful, but
use the smallest of drop
Kate Mendell
Histopathology/Lab Manager
HOWARD S. GOLDBERG, M.D., INC
990 Paradise Road
Swampscott, MA 01907
TEL: 781.595.0151
FAX: 781.592.6780
kmend...@goldbergmd.net
www.cosmesticdermcent
Hint when using these - do NOT try to fold them up into a nice looking
square. Once processed and in paraffin, it is very difficult to find the
edge, to try to open back up.
Fold into a not nice to look at, off-set square that is slightly crumpled.
Much easier to find the edge.
Peggy A. Wenk
Drop of hematoxylin on the tissue, when put on the paper in the grossing
area. Use a syringe. Only a SMALL drop. Too much means there's extra blue
all over the paper, making it hard to see the blue tissue.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
The opinions e
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