Hi,
My choice to air dry rather than dehydrate in ETOH & xylene is based
on the stain rather than the spooky xylene hazard boogyman. Yes, not using
xylene if it is not really needed is not a bad idea, but the main reason I
air dry some stains is the alcohols remove some of the stains. Ever hav
Thanks for sharing. This is enlightening. I wish I was making what you
suggest!Joelle
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: cont...@histocare.com
> Date: Tue, 11 Sep 2012 13:13:40 -0500
> To: one_angel_sec...@yahoo.com
> CC: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Histo
Toysha:
I think you have some confusions about what Lean is.
The fundamental premise of Lean is that "every step you do in the flow, will
"add value" to your product", besides eliminating "waste" or "muda" as is
called in Japanese.
If you go from stained section to cover-slipped section you are a
--
Well, actually that is something that I do not teach since that method is not
on the BOC exam. I only teach what is on the exam and usual methods in
laboratories. If the ASCP places it on the exam then I will cover it, but not
until then, they go by what is in
I think its safe to say I have never seen a 5 year tech make $40 a hour ( in a
hospital or privatly ). And I think its rude to post my comment back on the
forum when I sent it to you privatly.
I would be interested if all the other managers out there could please direct
us all to the $40 a ho
You do not need to "wet" the dried slides in xylene before using the automted
coverslipper.
René J.
From: Diana McCaig
To: E. Wayne Johnson ; Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; "Mayer, Toysha N"
Sent: Tuesday, September 11, 2012 1:23 PM
Sub
I have used Sakura film and Leica glass coverslippers (slides directly from the
oven)..
René J.
From: "Morken, Timothy"
To: Lori Harris ; E. Wayne Johnson ;
Rene J Buesa
Cc: "'histonet@lists.utsouthwestern.edu'" ;
"Mayer, Toysha N"
Sent: Tuesday, Septembe
EWayne:
All mounting media contains a so called "solvent". Permount contains toluene
(not as nasty as xylene) and will "penetrate" the section provided it is
absolutely dehydrated (in an oven in this case).
You just have to finish the HC (or IHC) protocol and pop-in the slides in the
oven.
Balsa
Does anyone know where I can find some large-head pins to use when pinning out
large tissue specimens for fixation? We already use the large "T-pins" and the
regular bulletin board push-pins are too small. We need something with a
larger, easier to grip plastic head, and long (at least ½ inch)
Hi,
Thanks for your response and the others as well. I don't really think it's a
lol situation here. I've seen uncertified professionals with good work ethic
and quality results get mid 20s easy. While they may provide a guide for lab
managers to refer to, thank God NSH doesn't set pay rates.
Dear Histonetters,
Would there be a problem with autofluorescence in bone when performing
immunofluorescence on formalin-fixed methyl methacrylate-embedded human
bone marrow trephines?
Thanks,
Orla
--
**
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bon
So you do not use as automated coverslipper?
Jeanine H. Bartlett
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.ut
We use tape coverslipping. Some techs dip the slides in the xylene on stainer
before adding them to the coverslipper and some just use the amount of xylene
coming out of the drip on the coverslipper. Either works fine and we have had
no problems.
-Original Message-
From: histonet-boun..
Would this work for auto cover slipping (tape film)if they were set in the
xylene reservoir prior to cover slipping?
Diana
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson
Sent: Septemb
Hello out there in Histoland,
I have to do some bulk runs with Pronase antigen retrieval. Up until now
the number of slides have been so few that I have been able to do the
antigen retrieval by dropping pronase on each slide and incubating them in
a humidified chamber at 37C for 20min. I now have s
If you dry the slides do you coverslip on a coverslipping machine - in which
you have to put into xylene to run?
Tim Morken
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori Harris
Sent: Tuesday, Septe
We have been using the oven dry method for special stains for about four years
now and it works wonderfully.
Lori A. Harris, HT (ASCP)
Histology Section Lead
GSRMC Pathology Lab
3600 NW Samaritan Drive
Corvallis, OR 97330
-Original Message-
From: histonet-boun...@lists.utsouthwestern.
I am convinced to give it a try because I also have trouble will the
loss of some stains in dehydration.
I was concerned that the slides would not clear well after oven
dehydration. I will see how it
works for me.
I can see clearly how going from counterstain to oven will save much
hassle wit
Toysha:
Perhaps you have not oven dried stained slides before, and that explains some
of your comments, like:
1- if the stained slides are completely dried, the "miscibility" you point out
is not an issues, because there is nothing to mix with;
2- if you dehydrate → clear the stained sections tha
Ooh, great question for my students next semester.
Your answer is the counterstain, some counterstains may require dehydration
after rinsing, or some may not. Adjusting the times of the counterstain is not
the issue as much as the solvent of the counterstain.
Rene, while I do acknowledge t
Hey Dorothy,
It's 10 years for CAP, but you need to check with your state as well. I
know for New York you must hold them for 20 years.
Thanks,
Tim
Message: 20
Date: Tue, 11 Sep 2012 08:09:24 -0700 (PDT)
From: Dorothy Glass
Subject: [Histonet] blocks and slides
To: histonet@lists.utsouthwe
CAP states 10 years for both slides and blocks. ANP.12500
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass
Sent: Tuesday, September 11, 2012 10:09 AM
To: histonet@lists.utsouthwestern.edu
Subje
Dorothy,
We are required by CAP to keep both our blocks and slides for 10 years.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com
Most states have their own regulations. I kept the blocks for 9 years and the
slides "for-ever" but after 9 years they were sent to a company that kept them
for us.
René J.
From: Dorothy Glass
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, September 11,
What is the number of years that you must keep blocks and slides for future
reference according to CAP and/or Joint Commission? I have heard 5 years for
blks, and 10 years for slides.
Dorothy R. Glass, BS,HTL(ASCP),IHC
___
Histonet mailing list
Histone
At my previous place of employment we used to separate the tissue from the
formalin. The STEL exceeded the PEL so we had to wear respirators. The
problem was that anyone entering the room was exposed. Based on this we
were able to justify having a outside company dispose of the waste. The
c
We incinerate the old tissue samples.
René J.
From: Tom McNemar
To: "histonet@lists.utsouthwestern.edu"
Sent: Tuesday, September 11, 2012 10:03 AM
Subject: [Histonet] Tossing old tissues...
Hello all,
A question came up during our last JCAHO inspection rega
Diana:
The most simple answer to your question is: "Because that is the way it has
been done for more than 150 years".
The second question would be: "Is it necessary?" and the short answer to this
question is: NO!!!
As a matter of fact, one of the steps I have developed to totally eliminate
xyle
I was hoping to get information on why special stains are dehydrated,
cleared and mounted vs allowing them to be blotted dry, air dried then
coverslip.
Every procedure I have ever encountered always indicates to dehydrate
and clear but I have heard where some labs are blotting the slides ,
allo
Dose anyone have a hair mount procedure they would like to share? This is
unprocessed hair that is mounted on slides. After time we get some major
airbubbles and thought of trying nairl polish around the edges, but thought I
would ask out in histoworld to see what you all are doing.
Thanks
_
We put everything in double red bags and add packets of Neutralex to equal
approximate amount needed. We do empty large containers, but I'm thinking about
stopping that as well.
We have Chemclav on site and have no known problems for the past several years.
j
Joyce Weems
Pathology Manager
678-
Hello all,
A question came up during our last JCAHO inspection regarding the dumping of
old tissues. Do you just throw out the intact specimen containers with
formalin and all into biohazard trash? Do you drain/collect the formalin for
other disposal and just place the drained tissues into th
Hi,
I am going to have some mouse hearts that have been fixed in zinc-Tris
fixative. I have never worked with this before. My question is about
processing. After doing some looking, I found an article that advised checking
the solutions on the processor , the first couple of alcohols , to be su
Looking for a small slide dryer, used preferably, for our School of
Histotechnology. Not a lot of counter space. Only 6 students, so don’t have a
lot of racks at any given time.
We have an old Shandon-Lipshaw rectangle metal “box”slide dryer right now, but
the insulation is going on it, so we n
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