Geez - why is everyone so touchy about picric acid? To be clear - we process
thousands of testes a year - we had to dispose of hundreds of gallons of
Bouin's a year - as well as try to store it prior to use. Residual Bouin's
fixed tissues were stored in 70% alcohol which was a pain in the
Kristen hope this helps. See CAP requirement for other than a pathologist
assistant or pathologist below. Our lab policy is to use the histotechnician
certification HT(ASCP) or HTL (ASCP) as qualification to gross since there is
an educational requirement for certification that is comparable
We are going to do special stains for iron (Perl's Prussian Blue for ferric
iron, Mallory's Method from WebPath: Internet Pathology Laboratory) and copper
(Rhodanine from Carson and Hladik) in mouse livers. We have on hand unopened
boxes of plus-charged microscope slides. Do we need to acid
For most histology demonstration of iron or copper, we are not doing a
quantitative analysis (exactly how much is in the tissue). We are usually
just demonstrating yes there is iron or copper, or no there is not. In the
case of hemosiderosis, hemochromatosis, or Wilson's disease, we might be
