Hello,
I have a puzzling artefact that I can't seem to correct in 7 micron frozen
sections of rat and mouse Small Intestine. When completing an IHC run, parts of
the section look great and the staining has worked fine - on other parts of the
same section, the morphology is ruined by the
It should label dendritic cells since it reacts with cytokeratin 8. These
cells have been shown to contain cytokeratin protein by immunoblotting. I use
this reactivity as an internal positive control.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director,
Hi,
I was hoping to get some input on the Thermo Scientific STP420ES Tissue
Processor (the one that looks like a washing machine). Is anyone using it?
Pros/Cons?
Mandy Bell HTL(ASCP)
Community Hospital of the Monterey Peninsula
2 Harris Court Suite B3/B4
Monterey, CA 93940
Hi Histonet-
I have to serial section rat brain slices through the entire block (5
blocks total per brain) for stereology. Since it is for stereology, I
obviously need to minimize tissue loss while sectioning. Does anyone
have any experience with using ammonium hydroxide to help with the
Greetings All:
For those of you who are planning to attend the National Society for
Histotechnology's Symposium/Convention later this week, I just wanted to remind
everyone that our group will hold it's only 'annual meeting' on Saturday,
9/29, from 12:00 to 12:45, (somewhere) in the
Good afternoon,
Has anyone used Albumin and Epcam antibodies to stain mouse samples? If you
have would you please share the antibody information and conditions with me?
Thank you,
Eva Permaul
Georgetown University
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Assuming that you are fixing fresh-frozen tissue sections:
Tissue is autolysing.
Use Formalin for 10 mins or 1:1 acetone:alcohol for 10 mins.
Imho, acetone is not a fixative..it's a delipidizer.
5 mins in that mixture is too short a time for the alcohol to be an effective
coagulant fixer.
I've been told that the HPV testing will be discontinued as of end of year. HPV
expresses the P16 gene but so does other type of cancers associated with HPV.
It is possible to have a P16 pos and HPV negative test.
But is it not possible to have HPV pos and P16 neg. if HPV is positive then
the
Eva
For mouse EpCAM I have used routinely during past 15 years G8.8 rat monoclonal
antibody from Developmental Studies Hybridoma Bank. Concentrated supernatant
(1:200 dilution for IF) works very well on 4% paraformaldehyde fixed frozen
samples (or on sections from fresh-frozen samples
Thank you Anatoli.
Let me add some more info to my question. The samples are FFPE.
Thanks,
Eva
On Tue, Sep 25, 2012 at 2:43 PM, Anatoli Gleiberman
agleiber...@cbiolabs.com wrote:
Eva
For mouse EpCAM I have used routinely during past 15 years G8.8 rat
monoclonal antibody from Developmental
We are trying to decide how to validate our stains when we switch from
Ventana's IView kit to their Ultraview Kit.
I have reviewed the CAP question on this and find the following wording:
The performance of new lots of antibody and detection system reagents are
compared with old lots before or
As far as lot to lot validation that's all we do. Use same control and compare
both.
Now validating a new detection kit is a whole different story. Here I just
made a checklist of all the antibodies we do and had the doc sign off on each
stain with the new kit.
If you want you can do a
Good afternoon Histoland! Anyone that is using Meditech AP module 5.6 please
grace me with your method of transcription/dictation for your Pathologist. We
just went live this year and it seems we have stepped back in time with this
system. The voice recognition system we use in Radiology is not
Hello,
I have been processing our tissue (derm only) in our Shandon Excelsior using a
xylene free process with Iso Alcohol. A few years back it was set up that way
by a Thermo rep and it worked really well. I am now having issues and the docs
seem to think it is a processing issue rather than
We are having a lively discussion about having 10 known positives and 10
known negatives to validate new antibodies. Many years ago we set up 5 and 5
even before CAP thought of the idea. This year's checklist added the 10 and
10 part, but it is up to the medical director.
What is everyone else
The NSH is upon us and time to meet, greet and exchange. If you are attending
and want to become involved in discussing and supporting Quality Improvement in
the HIstology Lab, then attend the Quality Control Committee meeting on
Saturday morning, September 29, 2012, 7:00 am - 7:45 am or stop
Hello, Histonetters,
If you are in the South (specifically TN, AL, AR,
MS, GA, KY),and represent or know of a laboratory that may be short
or inadeqautely staffed, I can help. I care about
timely patient care and helping to produce diagnosis as timely as possible.
If a need exists for a
Joe
I believe that is for initial validation, you can also place multiple tissues
on a slide. For new lot numbers of a previously validated protocol I would use
only 3 tissues, strong positive, moderate to weak positive and then negative,
these can all be placed on one slide. I'm not in
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