It also depends by the thickness of the pieces.
If they are small after about 20 minutes you can put them in new
paraffin where they will still stay for 20 minutes.
If the pieces have a medium thickness ( about a half of a cubic centimetre) it
would be better to keep them in the oven altogether
I have left them for over 24 hours with no ill effect on sectioning.
Demetria Ross deshsmi...@gmail.com 9/27/2012 6:21 PM
I'm curious to know how long can tissue stay on the machine in paraffin
before it becomes a problem I have left tissue stay in paraffin 30 min-2
hours before I take it off
It has been my experience that tissues that remain in paraffin too long (like
over a weekend) become brittle and hard. If we are embedding over 300 blocks,
those blocks may remain in the embedding station for up to 6 hours - but I
personally strongly recommend sticking to your SOP for
Anyone out there know of a caspase 8 that works well on mouse tissue?
Thanks!
-Liz
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Hello all,
We have an automated stainer we are looking to sell. It's a Microm DS50. If
interested, please email me. Thanks!
Adrienne
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Dear Colleagues
I would like to ask again about cell block preparation. of cause I found number
of answer on the histonet site and sorry that I ask again.
The cells what I prepare look distorted. Short protocol: 30 minutes of fixation
in 10 %NBF, centrifuge 1000 rmr, wash in PBS, centrifuge,
I want to know what is your preferred method for cutting paraffin blocks in
the microtome everyday. At work I am having issues with my supervisor
because we have different ways of doing things like for example she doesn't
like to use the technique where you first trim the tissue, cool it on an
ice
Freezing spray is better if you use it with discretion. Freezing too much could
render sections with artifacts
like partial holes on the section, so you must use it with care and not
always, as you stated, some
pieces cut better if you don't chill them at all.
The use of ice is possible,but I
Hi, I have a feeling that the supervisor's motivation for discouraging your
personal technique is financial and not procedural. I can't image in a high
volume setting where that technique would work, especially when there are
various kinds of tissue. Your supervisor's insistence on one way
