[Histonet] RE: Breast processing

This suggestion may not apply to reference laboratories, since these labs tend to work over the weekend. However, in our case, at an ambulatory primary care and surgical specialty clinic, I was able to work with our Ultrasound (Diagnostic Imaging) and ENT departments to have the breast core bio

Re: [Histonet] Facility Name on Slides

To my knowledge there is no regulation rather than having 2 identifiers,, Sent from my iPad On Oct 8, 2012, at 1:46 PM, "histot...@imagesbyhopper.com" wrote: > Does anyone have any regs that you can point me to for both Joint Commission > and CAP which details definitively whether or not a f

[Histonet] Tucson HT Position

Great full time opportunities' for a Histotechnician in Tucson, Arizona! Adobe Gastroenterology is looking for a certified HT or HTL and a Laboratory Assistant to join their new laboratory . HT candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1

[Histonet] Facility Name on Slides

Does anyone have any regs that you can point me to for both Joint Commission and CAP which details definitively whether or not a facility name is *required* to be included on slides? I know it's a good idea, the larger question here is whether or not there is a specific regulation for it? Tha

[Histonet] Histology Grossing Supervisor/PA Opening

Job Title: Histology Grossing Supervisor/Pathologists Assistant Location: Port Chester, NY area Schedule: Tuesday-Saturday9pm-5:30am Qualifications: BS/BA Degree with 1-3 years of supervisory experience. NYS License Pathology Assistant (ASCP) certification Interested applicants should email an upda

[Histonet] Metal molds

OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill Will

Re: [Histonet] Breast processing

My personal experience is that formalin fixation for 72 hours (and longer) does not affect immunoreactivity for ER, PR, and HER2 protein overexpression as long as the tissue does not "dry-out" before being placed in formalin and "thin" (2-3 mm) sections are submitted for fixation and histologic

Re: [Histonet] Brain - Question and advise

The original question was referring to "brain tumors", not to pieces of brain from patients with unknown diagnosis. A tumor is a diagnosis inconsistent with JK disease. René J. From: "McMahon, Loralee A" To: Rene J Buesa ; Histo Histo ; "histonet@lists.utsouth

[Histonet] Re: mounting medium

hey Tamara   We bought it.  I have heard that it polmerizes. But we just heated it and it is a retuned to its liquid state. Do you use your on wet or dry sections. I remain yours truely, Candice Camille From: Tamara A Howard To: candice_cami...@yahoo.com Se

[Histonet] Breast processing

Is there any evidence, that NBF-fixation for 72 hours alters Her2 staining, so that there's a diagnostic difference (pos./neg.)? publications with data? Personal experience? I have the feeling, that there is a mythos of overfixation. Anybody else ...? I saw turning up and down of Her2 results due

[Histonet] Breast processing

We have someone come in over the weekend and embed the tissue. If we go over the time protocol for some reason, which is not often, we will evaluate the specimen by FISH. Once you take the bitter pill of someone coming over the weekend, it works very well. Gary -Original Message- Fro

[Histonet] FW: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP)

Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net _ From: Hadi Yaziji, MD [mailto:i...@pathlearning.com] Sent: Friday, October 05, 2012 8:03 AM To: Pat

[Histonet] Fwd: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP)

FYI Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax ___

RE: [Histonet] Brain - Question and advise

I know but I have been in situations were the history of the patient is unknown or very vague, like vision loss or muscle tremors, then you process the tissue routinely...and then a few days later the 14-3-3 test comes across the radar. Just be careful. Loralee McMahon, HTL (ASCP) Immunohistoc

Re: [Histonet] question about decalicification end-point checks

What you describe (NBF fixation → band saw slicing → decal with formic acid → end point with 5% ammonium oxalate) seems a good protocol BUT you have to pay special attention to: 1- Did you fix the femurs "in toto"? If you did that, fixation is extremely difficult. You first should select a "gene

[Histonet] RE: SOX-10

Ronnie, I've not started using this marker just yet (I've pre-ordered it for mid Oct availability), but I just received a product sheet for Cell Marque's polyclonal rabbit ab which is available in concentrate or RTU If this interests you I can scan and email it to you (don't want to spam your in

[Histonet] SOX-10

Is anyone using SOX 10 in the diagnosis of soft tissue tumors? If so, can you please recommend a good reliable source for the antibody? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive

Re: [Histonet] Flourescent mounting medium- PLEASE HELP!!!

The sections should be wet (usually in PBS). If your mounting medium is a "little solidified" you should dilute it with the same diluent specified in the bottle or the MSDS René J. From: Candice Smoots To: Histonet Sent: Monday, October 8, 2012 9:42 AM Subject

Re: [Histonet] Brain - Question and advise

I do not worry about prions IF the tumor is perfectly classified as a metastatic neoplasm or any nervous tissue tumor. "Prions", as you know, correspond to a pathology with very well defined behaviors consistent with the human form of "mad cow disease" (KJ disease). That is why I recommended to g

[Histonet] question about decalicification end-point checks

Hi, I am looking for help with decalcification end-point checks. We use a 5% Formic acid decal (homemade - 1 L 88% Formic acid, 1 L 37% Formaldehyde and 8 L of distilled water). We do an endpoint check using 5% ammonium oxylate. Recently, we decaled canine femurs for a total of 13 days and en

RE: [Histonet] Brain - Question and advise

Curious as to why you wouldn't "worry about prions" ? Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@li

Re: [Histonet] Brain - Question and advise

If you will receive brain tumors, you should not worry about prions. On the other hand, you should ask the referring physicians the nature of the lesion to be on the "safe" side. The special procedural "tips" for brains can be summed up as: 1- make sure they are fixed correctly (at least 48 hour

RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!!

I have no experience with this particular mounting medium, but from what I gather it is like any other aqueous mounting medium. You should blot your slides to remove as much water as possible, without letting your sections dry. Then apply a small amount of mounting medium to the slide or coversl

[Histonet] Flourescent mounting medium- PLEASE HELP!!!

Hi Histonet     In our lab, we are trying to mount our flourescent stained sections with polyvinyl alcohol mounting medium with DABCO. WE are unsure if it is to be mounted while the sections are wet or do we let me air dry.   Another question is... How to actually use it. It looks to be a little

[Histonet] back online, please feel free to check it out

Dear Histonetters, Some of you visited the Facebook page to find some nice pictures instead of the CQPath site, which was disappointingly down. After a server crash this weekend, we are back online! Once again I want to kindly invite you to take a look at our IHC QC/QA/QI program. Please take