I clean them about every 10 days or so. I run them through the cleaning cycle
on my VIP 5.
O'Donnell, Bill billodonn...@catholichealth.net 10/8/2012 4:31 PM
OK folks, I know I should be smarter than this and I haven't seen
discussion on it lately
Are people cleaning their metal
We don't clean them after each use, but we do put them in the oven (in a filter
over a container) so the wax melts. Every so often, not sure of the frequency,
we clean in CitriSolv (our solvent instead of Xylene).
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for
Just one minor change in the below mentioned protocol. Do you really do
water washes? I have never heard of that. We do buffer rinses. (I
personally used Cacodylate buffer, but people also use Phosphate buffer).
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for
Dear All!
We processed the 3mm biopsies in peloris processor using factory 4 hrs.
xylene standard protocol.
But the processing was poor (soft) and almost half of the biopsies didn't
process well reagent status was ok.
Advised require regarding this problem.
Regards.
Muhammad Tahseen
Pathology
Yes, after each embedding session.
It is very simple: you just place your molds in the tissue processor in the
cleaning cycle and they will come out clean and sparkling.
René J.
From: O'Donnell, Bill billodonn...@catholichealth.net
To:
How long are your specimens fixed for before processing?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
tahs...@brain.net.pk
Sent: Tuesday, October 09, 2012 10:17 AM
To:
Dear all-
I have a user looking to label mitochondrial membrane.
Can anybody recommend a commercial antibody? I'm guessing one of the TOM
complex proteins would be a good target.
Thank you in advance.
Sho Fujisawa
Molecular Cytology Core Facility
Memorial Sloan-Kettering Cancer Center
415
We clean them every day. Ultrasonic bath with soap-water (dishwasher) at
40-50 degrees, 15 min. Paraffin swims on the surface. Molds are put out and
air dried.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
We clean our molds once a week. Soak them in Xylene to remove paraffin, soak in
100% alcohol to remove xylene, rinse in running water, dry and spray with mold
release solution.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Sho
There is a mitochondria marker for human tissue samples they are from Novus,
not sure if it's a membrane marker or not. Good Luck!
Protocol: Mouse anti Mitochondria
Clone: MTC02
Vendor: Novus Biologicals
Catalog Number: NB600-556
Species: Mouse
Isotype: IgG21
Spec Sheet: Novus
I am curious if there are any instituions that are using the Cellient
instrument for cell blocks on ECC cases that are scant or any surgical
specimen.
--
Kevin Schofield BS CT(ASCP)
Director, Outreach Clinical Operations
Yale University
Department of Pathology
(P) 203-737-2928
(F) 203-785-2641
We use this medium. We have found that if you shake it before use it becomes a
little more viscous, but toss it if it becomes too gel like. We also allow our
sections to dry, but keep them in the dark as much as possible. You also need
to be sure that you are using coverglass that works with
We haven't cleaned our molds for five years - if the blocks are cold enough
after embedding, there is no problem removing the block.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Does anyone have a Lot to Lot Verification QC form or procedure that they would
be willing to share with me for use with the Ventana Benchmark when comparing
lot to lots of antibodies and detection kits?
Thanks in advance for your help, Amy
Georgetown Hospital System
843-527-7179
NOTE:
The
Good morning all,
Could someone with more knowledge in this matter than I have help shed a little
light?
While at a nationally-renowned medical facility, I've come across something
rather interesting (to me) for which no one in the immediate lab has a
definitive answer for.
I see varying
I have use a mitochondria marker from Dako but it is cytoplasmic not on the
membrane.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From:
Yes, and that depends, as you note, on the residents doing the grossing.
Your solution: get the pathologists involved and find out how they want the
grossing to be done, and make sure that the pathologists communicate their
preferences to the residents.
It may be that the ones wanting to see
Talk w/ the pathologists or residents, have a conversation about what and why.
The multiple samples could be due to teaching or cancer protocols. The number,
size and selection area of samples is always the responsibility of the
pathologists. I find it best to not wonder why, just ask.
I have seen this phenomenon in several locations, not just with residents. The
thinking, as it was explained to me was that the little slivers were to be
embedded on edge like a cone biopsy to determine margins and that the entire
protstatic margin was to be submitted to be sure of complete
From: histonet-boun...@lists.utsouthwestern.edu on behalf of
histonet-requ...@lists.utsouthwestern.edu
Sent: Tue 10/9/2012 10:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 107, Issue 13
Send Histonet mailing list submissions to
Hi all, Has anyone tried doing muscle enzyme histochemistry on an automated
stainer? If so, I'd appreciate any information about your instrumentation and
methods.
Thanks!
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical
We occasionally use metal molds and we clean them after every use. We do this
by putting them into the tissue processor with the dirty rack during the clean
cycle.
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
--
Message: 9
Date: Mon,
Fawn,
Could you not use something like the number of recuts, reembeds, staining
repeats for something like that? For each parameter u could list
suggestions for improving those repeat test to lower the numbers from month
to month.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864
I always cleaned them daily, either the very hot water, soapy water method,
with water running over them in the sink with them on their sides so it passes
over them, not upright so the water sits in them- then a rinse in alcohol and
completely air dry. Or you can always do the clean cycle with
No sorry Tim, I have only ever do these methods manually. I'd be curious to
hear if anyone has automated these.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: timothy.mor...@ucsfmedctr.org
To: histonet@lists.utsouthwestern.edu
Date: Tue, 9 Oct 2012 17:51:22 +
Subject: [Histonet]
Position Title: MOHS Histotech
Shift: Monday-Friday/Full Time. This is a permanent/long term position
Position Summary
Performs histology and cytology preparation procedures on patient specimens
submitted for the diagnoses of disease.
Maintains specimen identity and integrity; produces high
Topic: cleaning holds
From:
histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Monday, October 08, 2012 2:32 PM
To:
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