[Histonet] RE: Uneven Staining

Liz, Could it be due to the presence of residual wash water or previous solution diluting the stain to give patchiness? On the same thread, insufficient agitation of the slides in the solutions? You do not say if staining is by hand or automated as this may be a contributor. Whilst I have no

[Histonet] RE: Uneven Staining

I have had this problem on a GI recut!! The first time through, the staining was perfect. The pathologist requested recuts, and I was cutting surgicals, so I put it in the back of the line up on my already melting ice bath to cut later. The recut was terrible. Nuclear staining was very weak!

RE: [Histonet] Waste drum labeling

I would also be interested in the answers to this. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From:

[Histonet] eosin in the processor

Hi Everyone, Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues.  I can't remember which station we put it in (I think it was the 2nd 100% ethanol).  Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am

[Histonet] Workflow Consulting Opportunity

Good morning, We have had a new position open with a global leader in histology. Please contact me directly at m...@personifysearch.com to learn more. Manager, Workflow Consulting –East The Company: Our client is a leading developer and producer of innovative high-tech precision

[Histonet] Protocol for preparing Cell Blocks

Hi Histo World, We are currently looking at how we process cell blocks and were wondering if anyone had a procedure they would be willing to share with us. Currently, we spin the fluids down in formalin and put the entire button in a biowrap and process that. This leaves our cell blocks very

[Histonet] MSI antibodies

Need help please..does anyone have a colon block, or would be will ing to cut me a few slides of colon, that has stained positive (which is actually no staining) for the MSI antibodies; MLH1,PMS2, MSH6, MSH2 ?   Thanks!   Kenia Lynch, M.T./H.T. Histology/Molecular Supervisor Caldwell

RE: [Histonet] eosin in the processor

The only immunostaining any residual eosin in tissue might affect is if you do immunofluorescence. Eosin does fluoresce. Others can address any effect on IHC. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] eosin in the processor

Eosin won't interfere with binding of antibodies, but eosin is fluorescent. You might want to consider that. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histologist need in Austin TX.doc

Histologist needed in Austin TX We have a full time day position opened for a HT or HTL (ASCP) registered histologist; requires 3 years experience and strong embedding and cutting skills. Experience in muscle enzymes, EM and IHC preferred. If you are interested in learning new techniques

Re: [Histonet] Waste drum labeling

With different products the toxicity level will vary. Write in the diamond the highest toxic level even if that is not the most abundant. By doing so you cover all inside the drum regarding their toxicity. I do not see the need for the emergency phone but if they told you to so so, do it. René

RE: [Histonet] eosin in the processor

Hi Kim, try 10cc of the prediluted Eosin (you use in H E staining) in your last 100% before your Xylene on the processor. By putting it in the last absolute it will not wash out. Your first Xylene will end up a little pink but your eyes will not strain as much trying to fine the tiny tissue

[Histonet] eosin on processor

Eosin on processor can be done with dirty eosin waste saved from the stainer; adding eosin powder sounds like a mess. Especially helpful for orienting small samples with a sidedness, skin GI GU oral, cornea No common down sides for routine HE, PAS, Trichrome, or IHC even with Red chromagens. We

[Histonet] Any reference labs offering CD52 (CAMPATH) on human FFPE specimens?

Looking for a reference lab offering the above. Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list

Re: [Histonet] Any reference labs offering CD52 (CAMPATH) on human FFPE specimens?

You might want to try www.phylogenyinc.com. On Nov 28, 2012, at 10:54 AM, Sebree Linda A lseb...@uwhealth.org wrote: Looking for a reference lab offering the above. Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI

[Histonet] Quick Question...

Hi Histonetters!! I hope you are having a great day. I have a quick question. Is ASCP certification required in order to work in the state of Michigan? Happy Holidays !! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare

[Histonet] Re: eosin in the processor

Supposedly eosin used to color small specimens, or put in the processor, fluoresces enough in tissue sections to interfere with fluorescent stains. I've seen safranin O (from the microbiology lab) used to mark small surgical specimens like GI biopsies. I don't know whether you can put it in the

RE: [Histonet] Uneven Staining - Update

This morning I decided to pH our DI water, which came out higher than it should. I then did an experiment where I cut a kidney and floated it on three water baths - one a bit basic, one a bit acidic, and one neutral. I cut two sections for each waterbath - one was picked up right away, and

[Histonet] Validation of a new tissue processor

Does anyone know what the CAP is requiring to validate a new tissue processor? Thank You Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential

[Histonet] Multiple Histology Mgmt/Supv Openings in NY, NJ, CT

Hope you had a great Thanksgiving! I am currently working on multiple openings in the tri-state area: IHC Supervisor- Suffern NY Lab Operations Manager (over cyto,histo, pre/post technical) Westchester Cty NY Histology Supervisor w Hospital Hartford CT Histology Supervisor

[Histonet] chemical disposal

Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing

RE: [Histonet] chemical disposal

It should be separate. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is

[Histonet] California HT Position NEED TO FILL ASAP

Great full time opportunity for a Histotechnician in Yuba City! North Valley GI is looking for certified HT or HTL to join their laboratory . HT candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/

Re: [Histonet] chemical disposal

Chemical hazardous waste. Sent from my iPhone On Nov 28, 2012, at 12:44 PM, Cynthia Pyse cp...@x-celllab.com wrote: Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous

Re: [Histonet] chemical disposal

You are right and your general manager is wrong (as they usually are when dealing with histology issues!). René J. From: Cynthia Pyse cp...@x-celllab.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 28, 2012 3:44 PM Subject: [Histonet] chemical disposal Quick question for

RE: [Histonet] Validation of a new tissue processor

You will also need to validate all of the IHC stains and special stains that you do on these tissues. You can take samples from each tissue type and run them in parallel on both machines. Then test all of your special stains and antibodies that you have in your inventory. :) Helen

[Histonet] microarray on OCT blocks

I have an investigator wanting to make microarrays from some frozen OCT blocks. I seem to remember reading a way to do this. Can someone please point me in the right direction? Thanks Donna Reynolds, HT (ASCP) Chief IHC Core Lab Dept. Cancer Biology, Univ. Tex. M.D. Anderson Cancer Center

RE: [Histonet] Validation of a new tissue processor

I don't think it is necessary to test every single stain or antibody you do to validate a new tissue processor. Most of the time the only thing changing is the machine, not the chemicals you use. The point of validation is to prove that the system gives you the same results as the old

Re: [Histonet] chemical disposal

Separate and chemical hazardous waste! Steven Mello,HT(ASCP) Sent from my iPad On Nov 28, 2012, at 4:48 PM, Rene J Buesa rjbu...@yahoo.com wrote: You are right and your general manager is wrong (as they usually are when dealing with histology issues!). René J. From: Cynthia Pyse

[Histonet] Job Opening

We are a small pathology in the Sacramento area (Placerville) looking for a certified histotech. This is a full time position that offers competitive compensation and full benefits. You can forward your information to Gary Martin at gmar...@marshallmedical.org.

[Histonet] short temp but home for Christmas

Hi All-   Looking for a happy person and a good cutter, preferrably the same person!    A short day shift temp, M-F from ASAP through the Friday before Christmas.  Fill up the bank account and be home for the holidays.  Experience with small biopsies required.  Full travel including per diems,