AB is a basic dye that is water soluble, it reacts with anionic groups. The
classic manual methods I checked use 30 minutes incubation times. What cells
is your pathologist wanting to highlight, mucins? The general method uses
acetic, and the methods for sulfated AMS, uses 1 N HCL. I guess we
I think the key phrase was that the pathologists expected a small amount of
cells to stain in the esophagus biopsy.
If your control has a lot of acid mucopolysaccharides (AMP) in it (such as
using a normal intestine, or lung with bronchus), then 10 minutes in Alcian
blue would definitely show
