AB is a basic dye that is water soluble, it reacts with anionic groups. The "classic" manual methods I checked use 30 minutes incubation times. What cells is your pathologist wanting to highlight, mucins? The general method uses acetic, and the methods for sulfated AMS, uses 1 N HCL. I guess we need to know if you are doing the general method and automated or manual- It might be a pH issue as much as the staining times. What is your control tissue?
Joelle Weaver MAOM, HTL (ASCP) QIHC > From: [email protected] > To: [email protected] > Date: Thu, 6 Dec 2012 20:58:10 -0500 > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue > and some say 10 min?Our control works well at 10 minutes but today I had a > Dr. say that he expected a small amount of cells to stain and they didn't in > an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've > read say 30 min, that's what would be best? > Thanks > Sheila > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
