Is anyone willing to share their staining protocol for Cresyl Violet stain for
HP
Thanks
Diana McCaig
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Hi Carole,
While I have no direct experience with human on human IHC, my first thought
would be to biotinylate your primary antibody and then you would omit the
anti-human secondary which is the most likely source of your background
staining.
If you are using fluorescence you could also
About 20 years ago in my lab they were developing an anti-human melanoma
antibody that we used to test on human melanoma (+) cases and we approached
more or less in the way you suggest and were able to eliminate background.
You just have to start testing, keep good records of what you do and
After a tour and review of the work-day expectations, the candidate completes a
simple math test (basically dilutions and percentage calculations) and then is
asked to observe and repeat the processes of sectioning and coverslipping.
Look for signs of disinterest (no questions - the wall of
I am a Certified Histotechnologist looking for temp or perm assignment within
30 miles of Newark DE.
I have over 25 years experience in all aspects of histoloy,
immunohistochemistry, image analysis and microscopy.
If interested please reply direct to the following email address:
Carole, Look at Golden Bridge International Labs. They have a human-on-human
detection system.
http://gbi-inc.com/
I've worked with them before and they have excellent products. They also supply
some products to other vendors that are rebadged.
Tim Morken
Supervisor, Electron
Bringing this up again, as it would be nice to have a definitive answer
about this. Will, do you have a reg number we can work with?
Thanks for the assistance!
Michelle
-Original Message-
From: Lynette Pavelich [mailto:lpave...@hurleymc.com]
Sent: Thursday, January 10, 2013 9:45 AM
To:
Hi Histonetters,
Good Friday to you all. Do you know if there is a “harder” paraffin that may
offer more support to the tissue while cutting? I currently use Paraplast Plus
and love it, but now I am cutting some polymer material and there is some
tearing and was thinking that maybe a different
Hello Betsy!
I use Leica's EM400 for my paraffin embedding needs and it does extremely well
for all of my decalcified bone work. Of course I mostly work with
undemineralized bone, biomaterials and medical device implants so I pretty
routinely utilize acrylic resins (MMA) for most of my work.
I'm in need of a Certified Histology Tech with grossing experience in the
Houston, Texas area.
Please contact me privately for more information.
Thanks,
Rachel
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We are going to be setting up a Leica 6025 Processor. Can anyone send me their
SOP and worksheets that you have created so I can use them as a reference and
modify them according to our usage. Would truly be appreciated as well as any
helpful tips or tricks you have encountered.
Diana
Why are you fixing ?
Try unfixed frozen?
Or, why not Pwax embedded?
They cut well in Pwax.
Sure, you may have to polish the cut surface with tissue damped in water/20%
alcohol to prevent cracking/curling just before each section is cut.
Or, after fixation, place in 30% sucrose until kidneys
Diana McCaig asked if anyone had a method for cresyl echt violet
staining of Helicobacter.
This information was published in Histo-Logic in April 1999. This was
all the information in the article.
Cresyl Echt Violet (CEV) This method utilizes a metachromatic dye,
cresyl echt violet (also called
Betsy,
Try the Paraplast Plus X-TRA. You can mix X-TRA with Plus (3:1) ratio
is too hard for your blade.
Good Luck!
madelein...@elcaminohospital.org
From: bmolin...@texasheart.org
To: Histonet@lists.utsouthwestern.edu
Date: Fri, 11 Jan 2013 17:31:02 +
CC:
Subject: [Histonet] harder paraffin
Good advice given.
Biotinylated primaries to be followed by stAvidin-488 or stABCpx-DAB, for eg.
Or, if biotinylated primary gives good result with above but, at low dilution
factor, use tyramide amplification?
Human- on- human can be just fine, depending on what proteins you want to
Colleen,
You can pre-warm your OCT block inside the cryostat @ -20c (~ hr) from
freezer before cutting, then gradually increase the temperature to
warmer with 1 degree increment (thickness dependant). I also
pre-chilled my slides before used.
Another alternative;
- Fix with 4% PFA/PBS for ~ 1 hr
Merk (Darmstad, Germany) used to manufacture paraffin waxes of 63ºC and 65ºC,
hard enough to be used in plant microtomy. They are all whitish.
Try contacting Merk, or perhaps Adrich.
René J.
From: Molinari, Betsy bmolin...@texasheart.org
To: Histonet@lists.utsouthwestern.edu
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