Not familiar with SubX but we have used Americlear for many years with good
results. You just can't beat Xylene for some things though and still keep a
little around.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
Both
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is intended for the sole use
Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in
other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw
this in one lab!?thanksIrena
___
Histonet mailing
We use Pro Par Clearant through Fisher from Anatech. Health-1,
Flammablity-2, Instability-0. For a case of 4 gallons it runs us about
$170.
Janine Simms Colon, CPhT, HT(ASCP), BHA
Histology/Pathology
Johnson Memorial Hospital
201 Chestnut Hill Road
Stafford Springs, CT 06076
Office: 860-684-8230
Thanks so much, everyone, for the feedback.
I have a colleague who is convinced paint thinner is exactly the same as the
SubX we use. Does anyone know if this is true?
Thanks again,
Adrienne
On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote:
Not familiar with SubX but we have used Americlear
Good morning, Wanda,
The answer is it depends. CLIA is pretty specific on what a cytotechs can
and cannot do. If your cytotechnologist qualifies as a cytology general
supervisor, then they could perform the function that you are referring. CLIA
493.1469, which refers to cytology general
I use XS-3 from Statlab for staining. Since it is substitute I had to do some
adjusting to the stain line but once I got it right I have had no problems We
pay about $70 for 4 gal.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Mark Tarango notes:
Many pathologists, if they have any doubt about the score will just say that
it is 2+ so that its gets HER2 by FISH which is considered the best method
for determining HER2 status.
On one busy pathology service I worked in 2004-2006 we were quite
explicit about overcalling
The best solution to eliminate xylene is to use isopropyl alcohol mixed with
mineral oil.
Xylene can be eliminated from staining by dewaxing with 2% aq. sol. of
dishwasher soap.
Before coverslipping oven dry the stained sections and cover directly.
To clean tissue processors use a 2% mixture of
Adrienne Anderson (where?) asks:
My lab is looking into xylene substitutes, and I'd love some feedback on what
other labs are using. We currently use SubX, but are there other items out
there more economical?
I never heard of SubX, but the Leica Microsystems Richmond [no kin!]
Inc. MSDS
Paint thinners are somatimes as dangerous as xylene is and less effective.
If you want additional information about eliminating xylene please read my
articles at
www.histosearch.com/rene.html
René J.
From: Adrienne Anderson rennie1...@yahoo.com
To: Tom McNemar tmcne...@lmhealth.org
Cc:
Good Morning-
Scenario: a specimen is received and has been in cytology fixative overnight -
a cell block is made from (very small) tissue picked out.
1.Can this be run on a program without formalin?
2. Does this need to be in formalin before going on the processor - if so
how
If you already have the cell block you can put it in the tissue processor. You
do not need to re-fix it, but it will when going through the formalin stations
in the tissue processor, and that OK and enough.
René J.
From: Nancy Schmitt nancy_schm...@pa-ucl.com
To:
We always have a different person make the review. In our situation, the
pathologist makes those decisions on whether it was miss.
CONFIDENTIALITY NOTICE:
This e-mail message, including all attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
Hi Cheryl,
We use the Aperio system for HER2 scoring. Our lab manager put a cytotech
in charge of validating the digital reading and her next project is ER and
PR. For HER2 IHC, the software is initially set for Dako's Hercept test.
We don't use Dako, we use Ventana staining platform and the
I would not vacuum the trimmings out of cryostat, if it were infected It would
make a good job of contaminating a laboratory etc.
Terrified Alan Bright
Sent from my iPhone
Hi,I wonder what is the way of removing shavings/trimmings from the cryostat
in other lab?, with the wet paper?
HER2 FISH really is considered the best method. It's the only method that
has actually been tied directly to patient outcome. The other methods are
expert consensus based. That being said, there are some cases that are
equivocal by FISH.
Sometimes we have to ignore the CEP-17 (green) signals
Happy Wednesday Everyone
There are vacumes specifically made for cryostats that are hepa filtered, etc.
We have one of those. The company is called MARMED. We use a shop vac for the
paraffin microtomes, that works nicely.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
Adrienne,
If you'd like information on Formula 83 please contact me off list at
b...@cbgbiotech.com
Thanks,
Beth Sell
CBG
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We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with
excellent results as well as cost savings.
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes
Hi Irena!
If you go to IMEB Inc.'s website, and type vacuum into the search, it will
take you to what we use. They sell a vacuum cleaner and accessories. We
currently use the filtered hoses with an old canister vacuum that's been around
longer than I have. When the filter gets full, we
If anyone out there is performing p16 on the Leica Bond immunostainer, could
you please tell me where you purchase your antibody and what protocol you are
using?
Thanks so much!
Kathy Maddox HT{ASCP}
Lake Charles, Louisiana
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Histonet mailing list
We have used Formula 83 from CBG for years with excellent results. We use it
for processing and staining. We also recycle it making the cost very low.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Hello All,
Please see the job posting below for a Histotech position at InCyte Pathology
in Spokane, WA. Only interested candidates should reply. Please do not
respond if you are from a recruitment service. Thank you!
Histology Analyst, Graveyard Shift (M-F)
InCyte Pathology is a
Hello all,
My lab is looking into xylene substitutes, and I'd love some feedback on what
other labs are using. We currently use SubX, but are there other items out
there more economical?
We use Clear-Rite 3 made by Richard Allan and is purchased at Cardinal. I do
not think it is very
Happy Wednesday Everyone
Has anyone been having consistency problems with their p16 staining since
MTM has been taken over by Ventana?
One day the procedure works another day it doesn't. Same procedure, no
variation in the temperature on pretreatment, run on the Dako 48, with no
errors.
Hi all,
What's your favorite anti-CD45 for mouse... Serotec rat monoclonal or something
else?
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
Notice:
I have a colleague who is convinced paint thinner is exactly the same as
the SubX we use. Does anyone know if this is true?
Haha- funny you should say that. I always swore that Mitaban (for canine
demodex mange) was made out of xylene. Almost looks like watered down xylene.
Well I checked
Hi Kathy, we do p16 on the Leica Bond. You have to purchase the p16 from
Ventana, they are the only one who has it. It comes as a predilute and we use
it as is with the DAB Refine Detection using retrieval solution ER1 for 10
minutes.
Joanne Clark, HT
Histology Supervisor
Pathology
Yes we monitor the temp and humidity. Retrieval is made fresh for every run.
We run the PT for the p16 in a water bath since we only run a few slides at
a time, but the water bath temp in also monitored. I should say that there
is some staining in these slides just not what the control usually
Could I get the opinions of any labs using the Leica Bond III for IHC staining?
I'm especially
interested to hear from those that switched from Ventana to Leica, but any
feedback is appreciated.
Few questions that come to mind:
Are you happy with the quality of the stains you're performing on
Any favorites for CD11b and CD11c for little mice FFPE tissue ? Has anyone used
the anti-CD11c antibodies grown in Armenian hamster?
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T-
Hi histonetters
Is ventana Ultra IHC only doing antibodies no FISH or CISH is this considered
High complexity testing? We are doing ER/PR and some others.
Thanks
Histology/Cytology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph
If you are just staining the slides and not reading them, then you are NOT
performing high complexity testing. The person who reads the slide is
doing the high complexity part.
Mark
On Wed, Feb 6, 2013 at 11:54 AM, Sara Baldwin/mhhcc.org
sbald...@mhhcc.orgwrote:
Hi histonetters
Is ventana
Please go to www.histosearch.com/rene.html to find proposals and discussions
about all commercially available xylene substitutes.
René J.
From: Parker, Helayne hpar...@skaggs.net
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 6, 2013 12:49 PM
This issue has been discussed at length recently (please go to HistoNet files).
The complexity does not deals with the actual test but with the ability of
the technician to go above and beyond the robotic tasks but also able to
think and apply knowledge when something goes wrong.
Sometimes
I would say this is high complexoty testing and the tech performing this has to
have knowledge of the process and troubleshooting in case there is issues with
the results. I do not agree with the interpretation some people give,, but
this is based on individual institutions
Sent from my iPad
Just to clarify, this is not my interpretation. This is what CAP will tell
you when you give them a call.
Mark
On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin jel...@yumaregional.org wrote:
I would say this is high complexoty testing and the tech performing this
has to have knowledge of the
The CLIA definition of High Complexity testing is not absolute, rather High
Complexity Testing is determined by a scored algorithm of the entire Test
System (preanalytical through Post Analytical).
As such, it clearly takes into account the laboratory or other personnel
performing all the
Hello
I am in need of prepared slides, stained for confocal microscopy or
unstained (DIC) of the following samples:
Primary
- non-lymphocytic leukemia
- renal (kidney) cancer
Secondary
- breast, bone, or brain cancer
- lymphocytic leukemia
- Lymphoma (non-Hodgkin's)
- gall bladder or liver
As always, thanks Tim!!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is
When we obtained the p16 antibody from mtm Laboratories we used it a dilution
of 1:10 on the Bond Max. I hope that the concentration will not change now that
Ventana is selling it.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Assistant Director, Anatomic Pathology
We have a small lab at a university in Beijing where we do diagnostic
histopath for swine diseases.
The lab is shared with graduate students who make histomorphologic
measurements on tissues like gut and
muscle as part of their research. We have a Sakura autostainer. Last
summer one student
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