[Histonet] Question for Alkaline Phosphatase / Biotin System Users

We are using standard avidin / biotin staining with alkaline phosphatase labeling and sometimes, not every time and not every slide in the same run we get residual blotchy spots and streaking of the reaction on and off the tissue. We have increased to two rinses after primary and two rinses after

RE: [Histonet] Waste Alcohol

The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax

RE: [Histonet] Waste Alcohol

Reagent Alcohol contains methanol, which is a U-listed EPA hazardous chemical and cannot be poured down the drain. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From:

Re: [Histonet] Waste Alcohol

You will probably want to contact your local or state office of environmental protection to find out what is required in your area.    The EPA lists hazardous wastes in 40 CFR 261.30.  Even if your waste is not on this list, it may have hazardous characteristics such as: ignitability,

[Histonet] streaks

Hi Claire,   I may be grasping at straws here, but I went to an NSH workshop once where this very question was posed.  You won't believe what the cause was.  They had a bad lot of blades.  The whole lot was nicked, so changing to a new knife or a new box of knives did not help the problem. 

[Histonet] Bob.1 SP92

Hi All I have a question about Bob.1 SP92 antibody, what is approximately the optimal titre for this AB from Cell Marque? Thanking you all for your suggestions. Robert Fauck Wellington Hospital, NZ This email or attachment(s) may contain confidential or legally privileged information

[Histonet] Gram stain on decalcified bone

I have a pathologist that wants a gram stain on decalcified bone. We are currently using the Twort's gram stain and the stain did not stain the bacteria in the bone sample, but the control tissue did stain positive. I was wondering if the acid in the decal solution could have affected the

RE: [Histonet] IgG4

We use Cell Marques Mouse monoclonal (clone MRQ-44) Cat# 367M-16 at a 1:200 dilution on the Ultra. We are using the Optiview detection kits. CC#1-24 min Option 5 - 12 min Antibody - 24 min OV Linker 8 min Multimer 8 min H2O2 +DAB 8 min OV copper 4 min Hematozylin II 8min bluing 4 min We

[Histonet] RE: Histonet Digest, Vol 111, Issue 37

Good afternoon Histonetters! We are currently looking for a qualified Pathology Assistant (ASCP Cert Required) for a full time position with a lab in Palo Alto, CA. Job description below; The Pathologists' Assistant (PA) dissects tissue specimens and provides dictated placement in plastic

Re: [Histonet] Gram stain on decalcified bone

Yes, they have been affected. René J. From: Shelly Christenson chris...@vet.k-state.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, February 26, 2013 4:11 PM Subject: [Histonet] Gram stain on decalcified bone I have a pathologist that wants a gram