Our alcohols that cannot be recycled (contaminated with xylene) are combined
with other flammable waste and dealt with by an external company.
L.White
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Hi
Has anyone ever tried to reprocess bone tissue that has been under
decalcified and processed through to wax?
Thanks
Fi M
Fiona Morrow
Dept. of Infection and Immunity
KFloor, Room K118
Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
0114 271 2102
Greetings,
once infected tissue is placed in fixative, Acanthamoeba form a double walled
encasement that stains nicely with any trichrome stain. Light green works
better than aniline blue. The amoeba are large and measure approximately 30
microns.
There are about 100 human corneal cases a
Go back to water (xylene or whatever clearant you use → graded alcohols →
water) and place in decal.
The results will be slightly of less quality than if the tissue was correctly
decalled in the first place, but you will be able to section it.
René J.
From: Fiona J Morrow
Either Gridley's method or an ordinary PAS will work very well, due to
the high concentration of glycogen in the amoebic cytoplasm.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph
Brooks
Sent:
Fiona
We have done this in the past we took the blocks back to water be melting them
out, re-cassetteing and putting through a cleaning cycle on the excelsior
processor then just decal as normal and reprocess, some hardening often occurs
but we can get sections of them suitable for reporting.
Our pathologists do it all.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org
-Original Message-
Don't forget to register for the Georgia Society of Histotechnology meeting
April 12-14.
What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us for
education,
networking, and some great scenery!
Wanda Simons
President of GSH
/bz
Augusta State University and Georgia Health
Dear histonetters,
March 1st is the ddeadline to submit any abstracts consideration for the 2013
Florida Society for Histotechnology meeting. To submit an abstract go to
www.fshgroup.org
Or email to f...@fshgroup.org
Jerry Santiago
FSH___
Histonet
