We noticed that once a section was placed on the water, the section has wholes
that appear. They are not present when sectioning (not seen by the naked eye)
but appear when the tissue is stretched. Is this an embedding issue? The
wholes appear next to the tissue. This hasn't happened before
Does anyone know about research or standards with this.
Deg C or Deg F
IB
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For the ALL INCLUSIVE price of $85, students my earn up to 15 CEU's through
NSH. The Georgia Society for Histotechnology offers FREE membership for
everyone. Robert Lott will be guiding you through the readiness for the HT/HTL
exams.
Find a roommate to split the cost of the room. If you
Hi everyone,
I have not had any success optimising the eosin counterstain in the absence of
xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking
sections through to this to absolute ethanol but then air drying , but it
leaves blotches of eosin. Using eosin 0.5% aqueous with
This exact reason is how I convinced my lab to go back to xylene!! I figure
it's because there is water in your substitute. Because you can't see if there
is water in it (from the air moisture or wherever...) it becomes frusterating
and annoying to have to change out to fresh solutions
Yes. Any more examples of near misses in histology and cytology? I will use
these case studies and source of errors as examples.
Although this may have been obvious human error with the wrong section on the
slide, a systems approach to quality improvement could have prevented this
incident.
We don't have problems with our eosin after Xylene-free deparaffining. I
use a 95% alcohol step just before the Eosin. Perhaps that might help.
We don't air dry before coverslipping as my prof finds the quality not
as good, so we use 100%IsOH before coverslipping; the mounting media you
use will
Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State
Gastroenterology Associates is a multi-physician practice located in Northern
Kentucky. Its mission is To provide compassionate, high quality,
cost-effective care to pateints with gastrointestinal problems Looking for
Oh, several types of problems I can think of in 30+ years of work:
Labels switched on slides from two cases. Slides labeled correctly at microtome
(hand written) but after staining the wrong paper label was put on the slide,
and the label that should have been put on the slide was put on a
Totally xylene free staining is a consistent sequence described as:
1- dewax the sections with 2 washes of liquid dish washer soap (any brand will
do) in a 2% aq. sol. at 95ºC of 1 minute each.
2- 2 consecutive 1 min. each wash with water at 90ºC and to water at RT
3- stain as usual, any routine
We use hot detergent solution for dewax and a xylene substitute (Pro-Par) after
staining without problems. If there is water accumulating in the clearant, it
can be removed with a molecular sieve placed in the staining trays. We don't
have that problem here in Montana because it is dry dry
We have the Hacker linear stainer, and for the past two weeks we had clean,
crisp HE staining. However, last Friday, something happened and our HE
stains no longer look crisp, but instead have a hazy, unreadable look.
Nothing different has been done, and we have troubleshot for every possible
If your acetic acid solution is made in-houseI would suggest remaking it,
just in case it was accidently made stronger.
Kari Kienitz HT, (ASCP)
Histology Laboratory
Portland Gastroenterology
The Oregon Clinic
NE 99th Ave
Portland, OR 97220
503.935.8311
kkien...@orclinic.com
How do I unsubscribe from Histonet??
Thank you,
Arjun
On Wed, Mar 27, 2013 at 1:46 PM, Kienitz, Kari kkien...@orclinic.comwrote:
If your acetic acid solution is made in-houseI would suggest remaking
it, just in case it was accidently made stronger.
Kari Kienitz HT, (ASCP)
Histology
I tell my techs who fancy themselves as fast workers that NO ONE will
remember how fast they were if they make major mistakes in the process. ONLY
the mistakes will be remembered.Tim I had to re-post, so true and really can't
be repeated enough. I had to learn this one the hard way
Years ago (30+) while working as a clinical lab tech in the Navy, the histotech
was thrown out of the histology department for doing sloppy histology work as
fast as he could, I volunteered to work in histology. After about 6 months of
OJT and some training at AFIP I returned to the hospital
We just upgraded our cryostat, and I'm wondering if anyone is in the market
for a used Leica CM 1900.
Let me know if you are interested.
deloris.car...@shawneemission.org
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Hello all,
I work in a medical lab that has recently expanded to histology and we are
working on our chemical hygiene plan. We have already done the initial air
sample testing for formaldehyde and xylene, but we were told by someone at OSHA
that we need to test for methanol as well. The only
Sophia,
Holes like you mentioned, are probably from the polymers or impurities in your
embedding wax separating. Gayle Callus mentioned this several times (see
histosearch), and recommended Stirring the embedding paraffin every so often
(before every use). It could also be your tissue
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