Re: [Histonet] Gomori Trichrome Staining

Hi, you can try, if you do not already, to wash briefly  in a solution of 0.2 ml. of glacial acetic acid in 100 ml. of distilled water, before the final dehydration. That makes the colors more delicate and transparent. The Gomori's trichromic results are similar to those obtained  with the Masson'

Re: [Histonet] Mohs histology hourly rate

Location matters. Where? Sent from my iPhone On May 14, 2013, at 6:10 PM, rook...@comcast.net wrote: > Hello, I was wondering if anyone knows what the going rate is for a Mohs > histologist (Hourly). I have 12 years experience as a histologist doing > routine histology and just recently got a

[Histonet] Mohs histology hourly rate

Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a job doing Mohs histology. Thanks in advance for any feed back, Christina ___

[Histonet] HPV In situ hybridization protocol (FISH or CISH)

Dear Histonetters, Does anybody have a protocol for HPV in situ hybridization (preferably high risk type probes). Preferably on ThinPrep cytological samples or similar. Regards, Mesru Turkekul ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Lysol IC for Ventana equipmetn cleaner

The dilution is listed on the bottle...I'm not at work but its something like 1:252. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mcken...@gastrodocs.net] S

[Histonet] RE: Bone marrow aspirate iron stain

Hi Jason, I am not familiar with the stainer but is the procedure starting from water (not dewaxing & re-hydration as is used for paraffin sections)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9

Re: [Histonet] Gross Room cleaner

Dispatch works well too. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie < amber.mcken...@gastrod

[Histonet] Gomori Trichrome Staining

Hello everybody, I am performing the Gomori Trichrome staining on frozen muscle sections (10um) but I have couple of issues: 1. The green staining is TOO dark; 2. When the green is not too dark, all my muscle fibers are red. I checked the pH of the Gomori solution, is 2.15. If anyone has some adv

[Histonet] Bone marrow aspirate iron stain

Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions? Jason R

RE: [Histonet] Re: [IHCRG] p40

Richard now the ab companies are really going to hate you advocating diluting predilute's further isn't that a sacrilege, what are we going to do with you, no negs and now diluting predilutes. I am just kidding I do it all the time myself. Cheers, Patsy Date: Tue, 14 May 2013 13:33:21 -0400 F

[Histonet] RE: Gross Room cleaner

Bleach =) Just make sure to rinse after cleaning or they will rust Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwester

[Histonet] Re: [IHCRG] p40

I have been very impressed with BioCare's "p40" monoclonal predilute. We are able to use it a 1:5 dilution on our Bond Max platform. Excellent immunoreactivity so far! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant

[Histonet] RE: Lysol IC for Ventana equipmetn cleaner

We use it but we only make it up 1 gallon at a time. We use 20ml to 1 gallon of distilled per Ventana. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-bou

RE: [Histonet] Formula 83: xylene substitute

Thanks, Linda.   What about the odor? Does it have strong or irritating odor? How did you staff responded to the change? We would like to know all before trying it out. Currenly, our staff is having issues with Xylene fumes and goal is to limit any fumes or exposure to Xylene.   I would like to

[Histonet] Lysol IC for Ventana equipmetn cleaner

I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! ___ Histo

[Histonet] Gross Room cleaner

What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mail

[Histonet] Lab Equipment: suggestions please

Any suggestions on a good microtome? Cassette printer, slide printer? Need to purchase and would love to hear suggestions of what's good & bad products to buy. Thank you Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Fw: [Histonet] p40

Hey Jim:   Just read the abstract you sent the link too.  Once again congratulations on yet another accepted abstract!  That was pretty much the jest of Davids' information and side-by-side images of p63 verses p40 on lung CA.   Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI

[Histonet] RELIA Histology Careers Bulletin. 5-14-2013. Exciting New Job Opportunities. I hope you had a nice Mother's Day!!

Hi Histonetters!! I hope you had a lovely Mother's Day!!! Can you believe it? Summer is almost here!!! My phone has been ringing off the hook this past week with some exciting new opportunities. This is a quick update of the positions I am working on that I am most excited about. Some are bra

[Histonet] Histology Technician Opening.

As of May 24th I will have an opening for a Tech. Days only 40 hours per week. No weekends doing routine histology, no IHC. Located in the beautiful Lakes Region of New Hampshire. * If interested here is the link: LRGHealthcare | Career Center www.lrgh.org/Jobs

RE: [Histonet] Formula 83: xylene substitute

Kiran, We use Formula 83 on both staining and processing without any problem at all. You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene. We also use CBG's recycler to recycle Formula 83 w

[Histonet] Formula 83: xylene substitute

Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any

Re: [Histonet] staffing

There is "no correct ratio" for HT to PT. That depends on the way the director of pathology runs the practice, the volume of work and the automation condition of the lab. You can go to http://www.histosearch.com/rene.html and read an article I wrote on staffing where you can find answer to your

[Histonet] staffing

Hi All, Does anyone have information regarding the correct ratio of Histotechs to Patholgists? I seem to remember getting that info at an NSH convention, but I cannot locate it. Thanks ahead of time. Laurie Laurie Best Histology Supervisor Sunrise Medical Laboratories 240 Miller Place Hiscksvill