[Histonet] costing UK only

2013-09-06 Thread Edwards, Richard E.
Hi All, I need to cost immunostaining etc., for a grant application, formalin fixed, paraffin processing, haematoxylin and eosin,5 antibodies, 50+ biopsies, using standard ABC kit, or perhaps the Envision package, any help or figures, or sources of figures and help gratefully

RE: [Histonet] Cell Block Preparation

2013-09-06 Thread Tom McNemar
This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. - Spin in a conical tube and pour off - Melt an agar slant (we get TSA slant from micro) - Pour the agar into the conical tube and spin for 5 minutes - The agar will

RE: [Histonet] Cell Block Preparation

2013-09-06 Thread Rathborne, Toni
I wonder if this method could be used with the product Histogel. Has anyone tried it? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, September 06, 2013 5:46 AM To: 'Ann

RE: [Histonet] Unsubscribe, Chapter 195

2013-09-06 Thread Manfre, Philip
Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax)

RE: [Histonet] Cell Block Preparation

2013-09-06 Thread Daniel Hewitt
We recently switched most of our cell blocks from agar to Histogel, works great. We use small disposable embedding mold, put the specimen in the bottom and add the histogel about half way up the mold, let it harden, pop it out and put it in the cass. Also cuts much better than agar. Daniel Hewitt

[Histonet] Cell Block Preparation

2013-09-06 Thread Webster, Thomas S.
This is a pretty good method for scant specimens. I have even used it for CSFs that have malignancy with success. http://www.jove.com/video/1316/cell-block-preparation-from-cytology-specimen-with-predominance CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the

RE: [Histonet] Cell Block Preparation

2013-09-06 Thread Dessoye, Michael J
We have had mixed results in the past with cell blocks, but currently we are filtering the fluid through a biopsy bag and processing that. We seem to be retaining more specimen with that method (we have used sponges and lens paper in the past). Michael J. Dessoye, M.S. | Histology Supervisor |

using celloidin RE: [Histonet] Cell Block Preparation

2013-09-06 Thread Morken, Timothy
Our cytology department uses a technique that involves coating a centrifuge tube with Celloidin then spinning the sample in that tube. The cellodin is then scored and the bag of celloidin containing the cell button is taken out and wrapped in paper to be processed in a cassette. It is a tricky

RE: [Histonet] Cell Block Preparation

2013-09-06 Thread Tony Auge
We spin the sample down, pour off supernatant. Resuspend in 5 drops thromboplastin and 4 drops human serum we receive from our phlebotomy lab. Let firm for 5 minutes and then fix in NBF. The agar suggestion probably works similarly if you can't get serum. On Sep 6, 2013 9:39 AM, Dessoye, Michael J

[Histonet] AUTO: Bruce Palmatier is out of the office (returning 09/09/2013)

2013-09-06 Thread Bruce_Palmatier
I am out of the office until 09/09/2013. I will be out of the office on September 5th. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For

[Histonet] Ki-67 (clone MIB-1)

2013-09-06 Thread ADESUPO ADESUYI
Hi, Please I am interested in pre diluted Ki-67 antibody clone MIB-1 that we can use on our Ventana XT and Ultra. I will really appreciate all the responses that I can get. Thanks, Adesupo Adesuyi Histology Supervisor Norman Regional Health System Norman, OK 73071