Hi All, I need to cost immunostaining etc., for a grant application,
formalin fixed, paraffin processing, haematoxylin and eosin,5
antibodies, 50+ biopsies, using standard ABC kit, or perhaps the Envision
package, any help or figures, or sources of figures and help gratefully
This is how we do it now. In the old days, we used agar and to my mind, it is
still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro)
- Pour the agar into the conical tube and spin for 5 minutes
- The agar will
I wonder if this method could be used with the product Histogel. Has anyone
tried it?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, September 06, 2013 5:46 AM
To: 'Ann
Trained professionals should know by now that if you want to unsubscribe, you
must type in all caps - UNSUBSCRIBE
Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486
215-652-9750
215-993-0383 (fax)
We recently switched most of our cell blocks from agar to Histogel,
works great. We use small disposable embedding mold, put the specimen in
the bottom and add the histogel about half way up the mold, let it
harden, pop it out and put it in the cass. Also cuts much better than
agar.
Daniel Hewitt
This is a pretty good method for scant specimens. I have even used it for CSFs
that have malignancy with success.
http://www.jove.com/video/1316/cell-block-preparation-from-cytology-specimen-with-predominance
CONFIDENTIALITY NOTICE:
This e-mail message, including all attachments, is for the
We have had mixed results in the past with cell blocks, but currently we
are filtering the fluid through a biopsy bag and processing that. We
seem to be retaining more specimen with that method (we have used
sponges and lens paper in the past).
Michael J. Dessoye, M.S. | Histology Supervisor |
Our cytology department uses a technique that involves coating a centrifuge
tube with Celloidin then spinning the sample in that tube. The cellodin is then
scored and the bag of celloidin containing the cell button is taken out and
wrapped in paper to be processed in a cassette. It is a tricky
We spin the sample down, pour off supernatant. Resuspend in 5 drops
thromboplastin and 4 drops human serum we receive from our phlebotomy lab.
Let firm for 5 minutes and then fix in NBF. The agar suggestion probably
works similarly if you can't get serum.
On Sep 6, 2013 9:39 AM, Dessoye, Michael J
I am out of the office until 09/09/2013.
I will be out of the office on September 5th. If you have a question about
an order or need assistance placing an order, or if the matter is urgent,
please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service
associate will assist you. For
Hi,
Please I am interested in pre diluted Ki-67 antibody clone MIB-1 that we
can use on our Ventana XT and Ultra. I will really appreciate all the responses
that I can get.
Thanks,
Adesupo Adesuyi
Histology Supervisor
Norman Regional Health System
Norman, OK 73071
11 matches
Mail list logo