Rui,
You will definitely want to consider using plastic media like methyl
methacrylate (MMA). It will cause less shrinkage in the tissue during
polymerization, you can still cut at a range of 4-12 microns using a rotary
microtome and tungsten-carbide knife, any mineralization present in the
Tim is right, fixation is the key to microwave processing. Size of the tissue
is very important for fixation, so be aware of that as well. Once you work out
the times per change of reagent the tissues should be just fine. To avoid
being concerned about the melted paraffin, invest in a
Georgetown Memorial Hospital is looking to hire an HT/HTL. This is a full time
position with hours varying from 37.5 to 40.0 weekly. If anyone is interested
and would like to apply please visit the Geogetown Memorial Hospital website
for details or if you would like to speak with me for details
Hi there, does anybody know the advantage of this stain over a straight
toluidine blue stain for wbcs??, many thanks.
Richard Edwards
Leicester university
We are currently using a Beecher MTA-1 tissue microarrayer and are wondering
1) if the equipment needs occasional calibration (and which vendors offer
this service)
2) if any vendors offer service contracts for this equipment in case
something breaks during routine use
I've looked into
Hi Histonetters!
Can anyone point me in the direction of an article that tests the stability
of DAB signal on a stained and coverslipped slide? There is a lot of
literature about the stability of the DAB reagent, but I am having
difficulty finding any reference to the stabiltiy of the
Hi all,
Does anyone have experience cutting human lymph nodes from a cadaver in
paraffin blocks? I am having quite a lot of trouble getting good sections,
even using a brand new blade the sections end up with multiple blade marks
streaking through the tissue and paraffin.
I've contacted the
