A brain artifact (vacuoles in the white matter) was noted in rat brain after
extended fixation. An experiment showed that rat brains fixed in 10% neutral
buffered formalin for 4, 10, 15, 20, or 25 days showed a progression of this
artifact starting minimally at day 10 and getting progressively
I would like to know if anyone out there has dealt with the new lica overnight
processor. now when a person is going to do a 2 day delay, now my understanding
is that if you do not press start this machine will not fill with formalin? but
yet I was told it does? can someone please answer this fo
We are currently recruiting for positions on multiple shifts for a client in
New York. New York lab license is required to be considered. If interested
please send me an updated resume, and as always a complete list of openings
can be found on our website.
1) Grossing Technician- 3rd shift
2) G
I think the problem with "a" is that 37% formaldehyde is what is considered
100% formalin not 10% nbf. So for all technical purposes I've never known of
anyone fixing samples in 100% formalin either But I would agree it depends on
what your doing with the sample if it got acetone or not. So i ag
Thank you for your information.
Since in our lab we have never used MMA and also no vacuum I decided to ask
histological service to embed the sample.I will section it and stain them with
Von kossa/Alcian blue by myself. The sample is being processed now and i will
see if the images of sample em
Hi all-
Has anyone had problems with the salt split method for DIFs not
separating? It has been sitting in 1M NaCl for 4 days now and is still
not budging. We do not do this method often and any suggestions would
be greatly appreciated.
Thanks,
Mehndi Helgren
Dominion Pathology Lab
Just make a quick calculation of how much "diluted" is the cost of the reagent
and you will realize that re-using the solutions is not worth it from the
monetary point of view, and much less from the quality aspect. Do not reuse
them.
René J.
From: "Berger, Re
We have been using the CD117 Rabbit Polyclonal from Dako (1:150) for many
years now, and it works very well on our Ventana platform. Haven't had any
problems with this antibody.
Thank you,
Cecilia M. Obregon
Memorial Regional Hospital
3501 Johnson Street
Hollywood, FL 33029
954-265-5317
The PMA-PTA can be reused, even when the color changes to green. I don't see a
problem in using fresh 1% acetic acid with every batch of slides? I just make
up a 1l bottle, or mix it quickly when doing the stain. Glacial AA isn't that
expensive, and it's no problem to pour down the drain (househ
In early 2012 we started using Cell Marque's rabbit mAb (clone YR145) for CD117
detection and we are very happy with it.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80
Hi Histonet,
Quick question about Masson's Trichrome. I'm staining several batches of slides
by hand, and normally only use the phosphotungstic/phosphomolybdic acid
solution, as well as the 1% acetic acid solution, just once and replace it each
time I do a stain. Does anyone out there re-use th
Elizabeth,
We get a rabbit monoclonal from Cell Marque, cat. # 117R, clone YR145 that we
are happy with.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: hi
We use Dako's polyclonal on our Bond III, diluted 1:400, catalog A4502.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chatfield
Sent: Monday, October 07, 2013 5:59 AM
To: histonet@lists.utsout
Hi Folks,
I'm looking for a new vendor for CD117. Up until now we purchased from Leica,
they no longer produce it.
Elizabeth
QEH
Charlottetown, PE
-
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