RE: [Histonet] RE: Dress codes

2013-10-09 Thread Jonathan Cremer
If you work with acids and bases (and other nasties) as in a synthetic chemistry lab, it's even a very good idea to wear cotton socks and trousers. Synthetics can melt into your skin when splashed with said chemicals, cotton will just be carbonized, giving you time to undress or run to an

[Histonet] P16

2013-10-09 Thread tahseen
Hi All, I am looking P16 for HP V in Sq.ca of oral cavity, which company and clone will be good. I use Ab cam pathologist not comfortable. Thanks, Muhammad Tahseen Supervisor Histology SKMCHRC Lahore Pakistan ___ Histonet mailing list

Re: [Histonet] Asbestos microtomy advice?

2013-10-09 Thread Rene J Buesa
If there are so many sites injected and so much injected, during trimming you may expose some fibers and maybe they can float in the air (?), and maybe you can inhale them (?) and maybe you can get asbestosis (doubtful) BUT if you want to be better safe than sorry, use a simple mouth/nose mask.

Re: [Histonet] Asbestos microtomy advice?

2013-10-09 Thread koellingr
Hugh, I agree with Rene about the possibilities and yes a mask might be overkill. But far, far, far more than that for me, the description of this research as stated bothers me greatly. I am surely no expert on this particular line of research but it is my understanding that projects looking

[Histonet] Pathology charges

2013-10-09 Thread Maray Weirauch
We've been told that before our pathologists can order/run any of these stains (CPT 88313, 88342 or 88360) on our pathology specimens, we must obtain a pre-authorization from the insurance carrier or the payment will be denied. Has anyone else run into this? How are you efficiently handling

[Histonet] RE: Pathology charges

2013-10-09 Thread Jesus Ellin
I did not know this was going on,, where is this being stated at -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, October 09, 2013 9:13 AM To:

[Histonet] Pancreatic trypsin IHC

2013-10-09 Thread Cartun, Richard
Is anyone using a commercially-available anti-pancreatic trypsin antibody for diagnostic IHC? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80

[Histonet] RE: Pathology charges

2013-10-09 Thread Weems, Joyce K.
I haven't heard of this. We do them on a daily basis and I have heard of no problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any

[Histonet] RE: Pathology charges

2013-10-09 Thread Blazek, Linda
I know you have to have the correct ICD-9 codes attached but have not heard of pre-authorization. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, October 09, 2013 12:13 PM

[Histonet] Immunostaining after prolonged formalin immersion?

2013-10-09 Thread Tim Wheelock
Hi Everyone: If tissue has been sitting in 10 % neutral buffered formalin for 3-5 years, can excellent immunostaining still be done on it. I am guessing that some sort of antigen retrieval technique can be done on the tissue, but I am not sure, since we don't do immunostaining ourselves.

RE: [Histonet] Immunostaining after prolonged formalin immersion?

2013-10-09 Thread Cartun, Richard
I have obtained excellent immunoreactivity for some proteins on various tissues that have been in formalin for more than a year; however, you won't know unless you try it and there are internal positive controls to validate negative results. Richard Richard W. Cartun, MS, PhD Director,

[Histonet] Negative Reagent Controls in Diagnostic IHC....

2013-10-09 Thread Cartun, Richard
My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?. I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who

[Histonet] p16

2013-10-09 Thread Gary Gracie
Dear Muhammad, We are using MTM p16. This is now supplied by Roche cat# 06594441001. It is only sold as a pre-dilute but I dilute 1/2 and it still works very well. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney Australia Hi All, I