Okay so my question is.how did you address the non- use if you
happen to be required? (Joint Commission)
Beatrice L. Sullivan
Corporate Histology Supervisor
Virtua Voorhees
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Richard,
I wish we could eliminate them but not only do our negatives displaying some
non-specific staining, seems to be tissue dependent, but our in-house QA people
say we need to continue using them per the data sheets accompanying many of our
antibodies. We use Ventana UltraView DAB
I normally purchase India Ink from one of our vendors, but know it's also sold
and used at artist supply shops. Does anyone know if I can used the artist
india ink for Pathology use? Really, the only issue would be if the ink stays
on the tissue during processing right? Please advise.
Ed
The only warning I know of and I have used India Ink is to be sure it is the
permanent India Ink not the washable. If you buy the non-permanent it will
come off in processing.
Pam Marcum
- Original Message -
From: Ed Crespo ecre...@cmblabs.com
To:
Hello to all in histoland. Is there anyone using the Novec 7100 fluid for
freezing frozen blocks in a low temp freezing bath. We were getting it from
fisher but they have discontinued it. I am trying to find another vendor. Any
help will be appreciated.
Allison Scott HT(ASCP)
Supervisor,
Hi Allison,
We use Novec 7000, but we also had to switch suppliers. Now it is purchased
direct through the 3M company. You will find it on their website.
Nancy Thomas
Stowers Institute for Medical Research
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Ed Crespo, CT(ASCP) in Cypress CA asks: I normally purchase India Ink
from one of our vendors, but know it's also sold at artist supply shops.
Does anyone know if I can use the artist india ink for Pathology? Really,
the only issue would be if the ink stays on the tissue during processing
right?
India Ink = India ink, no matter where you get it from.
rené J.
From: Ed Crespo ecre...@cmblabs.com
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, October 10, 2013 12:53 PM
Subject: [Histonet] India Ink for inking surgical margin borders
I normally
Dear Histonetters,
I am so happy to discover a new CD31 antibody on mouse tissues through
Histonet. It really works well. Now I need p16 antibody that is good for
FFPE mouse tissues.
Appreciate your help as always!
Regards,
Mesru
mskcc.org
___
Histonet
Tattoos Yucky? Guess I'm yucky. I got yucky way before tattoos became popular
when the response was more akin to fringe element.
Happy member of the Fringe.
:)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Hello Fellow Netters,
Has anyone tried using some type of external UV source to decontaminate a
Leica 1850 cryostat? I found out that it is not possible to retro fit the
1850 for UV. I would like to be able to avoid having to defrost, breakdown
and bleach the cryostat everytime a suspected
Hello;
Can anyone tell me if there are anti-rabbit polymers (secondary antibody) that
are conjugated to fluorchromes ? Or are the only polymers available conjugated
to HRP?
Susan Wert, PhD
Cincinnati Children's Hospital Research Foundation
Perinatal Institute/Pulmonary Biology
We are using them at Penn Medicine Dermatopathology. We interface with
our homegrown LIS. We like the printers. We have had some ribbon
issues causing the print to move and some numbers to be cut off and the
ink on the fisher slide can be wiped off so we are looking at other
slides to see if
I would like to see the PP slide. I was unable to attend in Providence.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: richard.car...@hhchealth.org
To: histonet@lists.utsouthwestern.edu
Date: Wed, 9 Oct 2013 20:58:38 +
Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC
We use the Slide Mate and we really like them. We did have one problem with
the printer tape and then we got a different lot and things have been much
better. The slides we use are the Thermo Colorfrost slides, in 6 colours and
we have had no issues with the ink smearing or coming off.
Pam
If you are a CAP accredited lab, CAP says that the cryostat must be
defrosted and disinfectant decontaminated at regular intervals with a TB
disinfectant.
- - -
ANP.23410 Cryostat Decontamination Phase II
There is a documented procedure for the routine decontamination of the
cryostat at
Help?
We are about to run out of AFB control slides and haven't had a good loaded
case in a while. Is there an easy way to come up with an AFB positive block or
could someone lend me one to be replaced at a later date? (Go through too many
to be cost effective to buy)
OR is there something
Sigma provides AFB control slides if you can't get block. The item number is
A2299
Terra Wineman, HTL (ASCP)CM
Research Biologist
636-926-7476 phone
terra.wine...@novusint.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I know source medical products has them cheaper than newcomer supply.
E-mail and ask for Walter.
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com ha...@histologistics.com
On Thu, Oct 10, 2013 at 5:24 PM, Cheryl tkngfl...@yahoo.com wrote:
Make your own.
Take some fresh lung (slightly edematous is better, if you can get it). Cut
into 2x2 mm cubes (or largest 3x3 mm cubes).
Contact Microbiology, and have them make a broth with a non-pathogenic AFB
in a large tube (e.g., plastic centrifuge tube). Put lung cubes in broth.
Hi Richard,
I would appreciate a copy of the power point.
thanks,
Andrea
On 2013-10-09, at 4:58 PM, Cartun, Richard richard.car...@hhchealth.org
wrote:
My colleagues and I presented a poster at the NSH annual meeting in
Providence recently titled, Negative Reagent Controls in Diagnostic
Paula,
A UV insert in available from Hacker Instruments. (1-800-4-HACKER) It was
not designed specifically for an 1850, but I don't see why it would not work
out for you. Give Jim Mullen a call, he should be able to give you the
device dimensions and power specifications.
Have a great day.
3M™ Novec™ Engineered Fluid HFE-7100 is made by 3M. They should be able to
tell you how to get it. I haven't had my hands on it yet for frozen
sections, but it's being very highly recommended.
Bob Richmond
Samurai Pathologist
Maryville, TN
___
Histonet
With due deference to Peggy Wenk, I'm a little hesitant about AFB and other
bacterial and fungal controls prepared by artificially inoculating normal
lung tissue, particularly with AFB other than Mycobacterium tuberculosis.
I don't understand why people don't use animal material here. Surely some
I use Artist
China ( India ) Ink.
The secret
is to immerse the blocks in Bouin’s Fixative to coagulate the Ink.
After that
put the tissue blocks directly in the First Alcohol.
Don’t go
back to formalin.
Cesar Romero
Buenos Aires
Argentina
Pam Marcum notes that The only warning I know of (and I have used India
Ink) is to be sure it is the permanent India Ink not the washable. If you
buy the non-permanent it will come off in processing.
India ink is a suspension of carbon black (basically soot) in a suitable
vehicle. It's quite
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