[Histonet] Remove OCT from frozen tissue block

2013-10-31 Thread Benoît Delatour
Dear histoneters, We got mice brains that were initially sucrose-cryoprotected and then embedded in OCT - flash frozen and stored at -80°C. These tissues were initially prepared for subsequent cryostat sectioning but due to technical considerations we need to cut them using a sliding microtome

[Histonet] re(2); 33

2013-10-31 Thread escott8
referral program http://homegate-ng.com/Scripts/TATI.php Sent: 10/31/2013 1:21:37 AM From escott8 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Remove OCT from frozen tissue block

2013-10-31 Thread Paula Sicurello
Hi Benoit, You can just let the OCT thaw at room temperature then blot away the melted OCT. To speed up the thawing process carefully cut away as much of the OCT as you can with a scalpel or razor blade and then let it thaw. I hope this information is helpful. Paula On Thu, Oct 31, 2013 at

[Histonet] Ideal Workflow Lab Design for Histo/Cyto lab Processes

2013-10-31 Thread Ian R Bernard
Histonetters, I'm looking for information or references concerning the ideal workflow design for a Histo/Cyto lab services. Need to consider ergonomics, work space, equipment, safety, etc. I know there are info. Just need source and experts to chime in. Journal of Histotechnology Advance

[Histonet] cell block staining issues

2013-10-31 Thread Martha Ward-Pathology
We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so).The

[Histonet] RE: cell block staining issues

2013-10-31 Thread Martha Ward-Pathology
Well actually it does help because we are trying to eliminate possible causes and it is nice to know that someone else uses Cytolyt with no problems. It is just frustrating because we are doing nothing different that I can find.The next step is to see if the samples are coming from the

[Histonet] RE: cell block staining issues

2013-10-31 Thread Tom McNemar
We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166

[Histonet] Thermo Slide Mate

2013-10-31 Thread O'Donnell, Bill
A note to vendors - I am in need of a roll of ink for the Thermo Slide Mate. I am told that our distributer is out and it will be 4-6 weeks wait. This is an unacceptable wait. Who else is making/marketing this product? I will gladly make a trial purchase. Contact me directly via email

[Histonet] 6 biggest problems with color brightfield images

2013-10-31 Thread jerry sedgewick
The link in the last email was incorrect. Here is the correct link: http://color-integrity.com/?p=61 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] 6 biggest problems with color brightfield images

2013-10-31 Thread jerry sedgewick
Just in case you're interested, I have written an easy-to-follow guide on 6 problems with color brightfield images, and 6 solutions. See the posting at a blogsite: http://color-integrity.com/?p=15 Enjoy, Jerry Sedgewick ___ Histonet mailing list

[Histonet] StainMate Stainer

2013-10-31 Thread Hans B Snyder
Hello, Does anyone know if the Stainmate stainer can be programed with 50 steps or more? I have looking to buy one but need it for a purpose other than cytology and histology. It needs to be have 50 steps or more for this application. Thank you Hans B Snyder Histologistics 60 Prescott Street

[Histonet] RE: Ideal Workflow Lab Design for Histo/Cyto lab Processes

2013-10-31 Thread Lori Harris
I would also be interested. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

[Histonet] Specimen Tracking Systems

2013-10-31 Thread Matthew D. Roark
What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... Any help and recommendations would be appreciated! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical

[Histonet] Pancreatic tryptase

2013-10-31 Thread Cartun, Richard
I may have asked this question before, but, if I did, I did not receive any replies. Is anyone doing IHC for pancreatic tryptase and, if so, where do you get your antibody? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen

RE: [Histonet] Specimen Tracking Systems

2013-10-31 Thread WILLIAM DESALVO
My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to

RE: [Histonet] Specimen Tracking Systems

2013-10-31 Thread Morken, Timothy
I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND