[Histonet] HE stainer

I have a Leica autostainer that is capable of several different programs, including dehydrating/rehydrating. I use it to counterstain my IHC so the hematoxylin does not mess up my IHC stainer. I like this stainer because it is easy to use and maintain. Kathy Machado,HTL Danville Regional

[Histonet] PicroSirius Red in Frozen Sections

Hello! I did a Picrosirius red stain using the polysciences kit in paraffin section cut at 4microns. The stain looks great. Later on my boss asked me to do the picro using the same kit but in frozen section. The stain looks very, very intense. Sections are really red. When looking under the

[Histonet] Leica vs Ventana

Hi Mehndi. I hope this is still useful information. I wanted to let you know that I am a faithful Ventana user. I have been using strictly Ventana IHC stainers for several years now. Currently we have two Ultras. They are very user friendly and the tech support is awesome. We looked

Re: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work?

I really do not know of any study comparing -20ºC vs -80ºC or if this -80ºC is the result of a commercially driven  ideal storage method. I advise you to check about any bibliographic references BUT meanwhile I also advise you to keep your precious and irreplaceable material at -80ºC. I do not

[Histonet] FT Histotech Job

FT Histotech fo a GI Path Lab in Northern California Great pay plus benefits Must be license/certified Experience of at least 2 years Embedding/special stains/processing Self motivator ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Setting up ASR antibody to meet CAP requirements

For the first time my pathologist would like to bring an ASR antibody in house. It is made by Biocare, and our validation slides look good compared to the other clone we want to get rid of. However, I see in question 12425 of my checklist that I have to establish or verify the performance

Re: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work?

Hi David, An institute that I work for would take the samples out of the -80 and place them into a -20 before I cut them, sometimes this would be overnight other times several hours. We were experiencing freeze thaw artifact on those samples, when asked why they were doing this, they thought

[Histonet] Immediate Open Position in Gig Harbor, WA

Hi All, A Dermatologist in Gig Harbor, Washington is looking for a full-time HT or HTL. Candidates must meet CLIA compliance requirements to perform gross. Ability to perform Mohs is a plus but in-house Mohs training is available. Anyone interested in more information may reply to this

[Histonet] Leica vs Ventana

Our hospital facility, as well, are faithful Ventana customers. We have 3 ultras and appreciate the ease and flexibility needed for a busy hospital facility. The slide drawers are random access so the slides are staining independently from one another. Another great feature of the ultra is

RE: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work?

The following (from The Book - that may never be published!): It is also important to remember that -20oC is not suitable for storing frozen tissue. Have you ever noticed that meat stored too long in the freezer suffers freezer-burn; it appears dried-out? The condition is primarily caused by

Re: [Histonet] PicroSirius Red in Frozen Sections

I'm going to try to take a stab at this, but I don't know specifically about the picrosirius red stain. I'm going to talk in general about stains and fixation (or lack thereof in the frozen section (FS)), and the relationship between fixation and staining. I'm going to start out very simple,

[Histonet] Supervisor position, Histology, UC San Francisco Medical Center

The Histology Supervisor position has opened at UC San Francisco Medical Center in San Francisco, CA Some basic info: Supervises Histology, special stains and IHC. 13 histotechs 30 pathologists, 25 residents, 3 certified PA's, 6 histotech PA's Average 500 blocks/day, range is 300 to 900 Recuts