Just a thought - have you tried changing the angle of the blade?
Each different type of blade from different vendors need a different angle
for microtoming.
Each different type of microtome from different vendors need a different
angle for microtoming.
I know these two facts, as I have
Are you using tape or glass coverslips? We have found that when using tape
coverslips, at least two very clean 100% alcohol steps must be added before
slides go into the clearing reagent, be it xylene or Histoclear. If not, brown
spots appear on some tissues, usually liver and bone. If
So far when I ask about neuro stains being automated by any company the answer
is no. The volume clinically is not high enough and the times required would
tie the stainers up from hours to overnight in for some steps. It would almost
require a dedicated stainer for neuro only and would be
I was just wondering if anyone was having difficulty cutting biopsies? We have
been using the same blades for years and now it is so difficult to get a
ribbon. There are three other area hospitals having the same problem. I have
been trying samples from various vendors and the problem is
I wouldn't be too quick to blame the blades. Have you changed paraffin
brands/temperatures or changed any of your processing schedules? Is the
fixative/fixation process the same? Is the collecting location doing anything
different/have they had a change in staff?
Also, what type of blades are
I use McCormick Paraplast Plus.
On Tuesday, November 12, 2013 8:14 AM, Goins, Tresa tgo...@mt.gov wrote:
Paraplast X-tra by McCormick.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew D.
We stumbled on Polyscientific's GemCut Pink Sapphire Parrafin and really like
it. Before that we used Paraplast and it was OK but our blocks cut so much
nicer with the GemCut.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Is anyone using the Thermo/Microm Secton Transfer System on their microtome?
http://cellab.se/pdfbroschyr/Microm/STS.pdf
I'd like to get some input for using it to cut serial sections of microarrays.
Thanks!
Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San
Our immunofluorescence protein antibody stains are automated, but not the
routine histochemical stains used for muscle biopsies. Between the importance
of maintaining pH levels, incubation temperatures (SDH,NADH,COX, AtPase's,
Amyloid, MAD, Myophos, Acid Phos) and the need of a rotator plate
I would also like to know
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Thursday, November 14, 2013 9:55 AM
To: Histonet
Subject: [Histonet] Anyone using Section Transfer System by
Hi everyone,
Has anyone had any problems with the Immedge Pap pen from Vector. Cat # H-4000
It gives a nice wide barrier, but for some reason the barrier is disintegrating
on our slides so I get holes in the lines I draw.
I have had a similar problem before, usually because the slide wasn't
Tim,
HM340 + STS now successfully uses in
many Russians hisology labs and techs
loves them.
STS have the one disadvantage: is need
to much time for cleaning and
maintenance vs waterbath.
Also it is necessary some skills for
using it.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
Hi Patrick,
yes, I had a similar problem with the Immedge Pap pen. I took very long before
I could immerse the sections again with buffer and the barrier still collapsed
later on. I used a quite old one that I found in the lab so I guess they stop
functioning after a while.
Klaus
Klaus
The problem is using the pen without redistributing the liquid and solid
contents in the pen. Years ago, the Vector technical service rep said to
shake the daylights out of the pen. We use a vortex mixer, at highest
speed and press hard, to make sure the contents were well mixed before use.
We
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