[Histonet] GMAfix.

Hi All, a Happy New Year to all, my question is, is there any point in including iodoacetamide and phenylmethyl sulphonylchloride in the acetone( used at -20C) used for fixing biopsies prior to GMA processing/embedding?, many thanks.

Re: [Histonet] Frozen section protocol on rat tendon and/or muscle as well

Dear Peter: Our muscle freeze protocol is very easy. We employed a small piece of cork of about 1x1cm, we pour a drop of OCT embedding medium and we placed the muscle on it. It´s important not to pour a big drop of OCT, cause it creates holes in the muscle as it infiltrates. Then we cooled

Re: [Histonet] Frozen section protocol on rat tendon and/or muscle aswell

Is the -80 degrees really a correct temp? We freeze our muscles around -150 to -160 Degrees C. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Ignacio Ruz Caracuel Sent: Thursday, January 02, 2014 7:27 AM To: Peter Petro ; histonet@lists.utsouthwestern.edu Subject: Re:

Re: [Histonet] Embedding paraffin for brain tissue?

Hi there,   1. Have you changed anything in the processing and embedding processes themselves (dehydration, clearing agents and times, etc)? Friable sections could also come from extended times in the clearing agent...   2. There is also a section transfer system with a waterfall. We have a

[Histonet] cryostat

Does anyone have any current information on the cryostats with UV for decontamination? Specifically, is it accepted as a means of decontamination by CAP or JS or any other regulatory agencies? Basically, is it worth the extra dollars to go that route? Much thanks and Happy 2014!! Dorothy

[Histonet] RE: Histology program/school?

Darton College in Albany has an online associates program. I would start there. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments

[Histonet] RE: cryostat

ANP.23410 Cryostat Decontamination Phase II There is a documented procedure for the routine decontamination of the cryostat at defined intervals, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal

Re: [Histonet] Histology program/school?

Is any one knows any school/Lab in Europe? On 02 Jan 2014, at 18:01, Weems, Joyce K. joyce.we...@emoryhealthcare.org wrote: Darton College in Albany has an online associates program. I would start there. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax

Re: [Histonet] Histology program/school?

For Schools in the British Isles: http://www.nhshistopathology.net/ Click on Schools. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Leila Etemadi Sent: Thursday, January 02, 2014 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histology program/school?

[Histonet] Betagalactosidase/LacZ

I have a question Lac Z staining and BetaGalactosidase IHC. The Lac-Z stain is enzyme dependent, so it must be fresh/frozen for the reaction to work. Is this also true for the betagalactosidase IHC? The target of the antibody is the enzyme, but I am not sure if the enzyme need to be active

[Histonet] Fixed tissue storage ratio in 70% ethanol

Hello, When transferring formalin-fixed tissues to 70% ethanol for storage, is there a recommended ratio of ethanol volume to tissue volume? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA

[Histonet] Beta-Amyloid antibody, clone 6E10 availability

So Histonet land, our usual vendor for this antibody can no longer get this clone. My question is: does anyone know who might hold the patent on this clone or at least know any company selling the 6E10 clone? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH

[Histonet] RE: Beta-Amyloid antibody, clone 6E10 availability

Linda, We get ours from Covance. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Embedding paraffin for brain tissue?

Hi All Before looking at the wax issue, have you tried changing/looking at the processing issue first? We cut our human brain tissues (routine and large ) daily and we don't have any problem . We found that brain specs are better processed on a 5 days cycle, making them easier to handle ,cut

[Histonet] Hot Topic Medicare Code

HAPPY NEW YEAR HISTONETTERS! Ok, so here is a question for 2014 coders.How do you code ( or should I say get to code) a doublestain for Medicare and for non-medicare? G0461 and G0462 (Medicare) and 88342 and 88343 for non-medicare. Can you charge for both antibodies in the doublestain

RE: [Histonet] Hot Topic Medicare Code

AMA prescribes codes 88342 and 88343 for qualitative IHC (Non-Medicare): The AMA's CPT-2014 codebook offers two codes for reporting with qualitative immunohistochemistry (IHC) testing. The codes and official parenthetical instructions for their use starting Jan. 1 are as follows: 88342