[Histonet] RE: TAT for Transplant Biopsies

We accept biopsies until 10:30 a.m. Monday through Friday. If it is an extreme emergency, we will accept a later cut off time and someone stays to take care of it. Yes, we will process transplant biopsies on weekends and holidays. We do not process the biopsies at night. Hazel Horn

[Histonet] Frozen sections

Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why?

RE: [Histonet] ROS-1

You might try contacting Cell Signaling Technology in Danvers, MA. They carry a rabbit mAb to ROS1 and I believe they have control slides that can be used for validation purposes. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

Re: [Histonet] controls

Is a candid control one that's taken when the fungus doesn't know you're sampling it? Sincerely? Jay A Lundgren, M.S., (HTL) ASCP On Fri, Jan 10, 2014 at 11:58 AM, Webb, Dorothy L

[Histonet] Processing:

I have one tech telling me that when the entire processor is changed the tissue is too dry. We run a lot of fatty tissues, breast, etc on this processor. (Our biopsies are run on a separate processor). Is this correct, or should we only rotate reagents? No other techs complain. I have a hard

Re: [Histonet] Processing:

This depends on so many different factors, however, I prefer a frequent rotation over a complete change. Do what is best for your tissue! Sent from my iPhone On Jan 13, 2014, at 9:46 AM, Jb craiga...@gmail.com wrote: I have one tech telling me that when the entire processor is changed the

RE: [Histonet] Processing:

This gets me back to another recent topic, soaking the blocks. I've seen this a little in the past, just soak them on an ice block,tray for a couple minutes and you'll be fine. To me, another indicator would be that if you're getting dry tissue when changed but not later could there be some

[Histonet] myeloperoxidase on FFPE or frozen sections

Dear Histonetters, I wonder if anyone has done myeloperoxidase stain on FFPE or frozen lung sections? I would appreciate any help. Regards, Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] per diem tech in Boston area

Fellow techs, I have a per diem histo tech position in the Boston area. We are a small uropath lab and the duties will include embedding, sectioning and IHC's. thanks, Ron Martin ___ Histonet mailing list

[Histonet] microwave processing

Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there

[Histonet] RE: Frozen sections

Our pathologists prefer to perform all aspects of frozen section preparation. We will stain for them, but they would rather gross and section themselves. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] microwave processing

Perhaps you can get some literature from one of the vendors that sell that technology. Milestone Medical Sakura Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab)

[Histonet] RE: Techs grossing FS tissue

This is the only way that a non-pathologist can gross tissue, whether for frozen section or otherwise. They must meet CLIA standards for high complexity testing and furthermore, CAP says that the exact nature of the tissue grossed must be spelled out, along with nature of the pathologists'

RE: [Histonet] microwave processing

Gudrun, A good overview is here: http://www.ebsciences.com/papers/mw_tech.htm A couple old books: The Microwave Cookbook for Microscopists, Boon and Kok Microwave Applications in Pathology [Hardcover] Anthony S. -Y Leong This person also wrote a lot of articles back in the 1980's and

Re: [Histonet] microwave processing

Hi Gudrun: I recommend you to get The Microwave tool book by Login and Dvorak (1994) I am also sending you under separate cover an article I wrote on the subject. As to your questions, the practice of histology has concluded that: 1- the physical principle is that microwaves excite (shake) all

[Histonet] Buehler Isomet 2000 Precision Saw or later model

Actively looking to purchase - possibly two, in good condition. Thanks Vicki @ 608-262-8534 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] myeloperoxidase on FFPE or frozen sections

Yep, We use a rtu from Leica on the Bond3 platform (PA0491) with EDTA (high pH) antigen retrieval Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University

[Histonet] Recuts/deepers

Hello histonetters! Could I please get some help/advice/ideas on how to reduce recut/deeper requests? What does your lab do to reduce that? Would you say embedding is partially the problem? Such as small tissue embedded with bigger tissue? Etc?

RE: [Histonet] Recuts/deepers

What are the reasons they ask for recuts? The answer to that will give you some ideas. For instance, did they not get a full face with margins the first time? Was the section not readable? Was the stain inadequate? Tim Morken -Original Message- From: